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HILIC HPLC and UHPLC Columns Information
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HILIC Overview
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Advantages of HILIC
Advantages of HILIC
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HILIC Overview
HILIC Stationary Phases
›
HILIC Retention Mechanisms
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Advantages of HILIC
›
Achieve better separation efficiency under highly aqueous conditions
Retention of hydrophilic compounds that are difficult to retain by Reversed Phase Liquid Chromatography (RPLC)
Improved sensitivity with MS detection (due to more efficient desolvation of the analytes)
Compatibility with SPE extracts which are often in 100% organic solvent
Low back pressures due to the low viscosity of the organic-rich mobile phases
Separation efficiency of very polar analytes in HILIC better than in RPLC under highly aqueous mobile phase
Comparison chromatograms
Column:
Thermo Scientific™ Hypersil GOLD™, 5µm, 150 x 2.1mm
Mobile phase:
H2O/ MeCN (98:2) + 0.1% formic acid
Detection:
+ESI (spray conditions adjusted for higher aqueous content of mobile phase)
Injection:
1ng on column
Column:
Thermo Scientific™ Hypersil GOLD™ HILIC, 5µm, 150 x 2.1mm
Mobile phase:
Ammonium formate 50mM pH 3.5/ MeCN (10:90)
Detection:
+ESI Injection: 1ng on column
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