Advantages of HILIC

Achieve better separation efficiency under highly aqueous conditions

  • Retention of hydrophilic compounds that are difficult to retain by Reversed Phase Liquid Chromatography (RPLC)
  • Improved sensitivity with MS detection (due to more efficient desolvation of the analytes)
  • Compatibility with SPE extracts which are often in 100% organic solvent
  • Low back pressures due to the low viscosity of the organic-rich mobile phases
  • Separation efficiency of very polar analytes in HILIC better than in RPLC under highly aqueous mobile phase

Comparison chromatograms

Comparison chromatograms
  • Column: Thermo Scientific™ Hypersil GOLD™, 5µm, 150 x 2.1mm
  • Mobile phase: H2O/ MeCN (98:2) + 0.1% formic acid
  • Detection: +ESI (spray conditions adjusted for higher aqueous content of mobile phase)
  • Injection: 1ng on column