HPLC and UHPLC System Components

The right liquid chromatography components for your application.

The right liquid chromatography components for your application

Select innovative and intelligent components that are right for your Thermo Scientific HPLC or UHPLC system and application. All Thermo Scientific Vanquish HPLC and UHPLC systems are made up of four main types of components. Choose from a broad selection of pumps, autosamplers, thermostatted column compartments, and detectors. Whether you require a nano, capillary, microbore, analytical, or semi-preparative flow configuration, our liquid chromatography components ensure reliable, precise, and accurate operation.








Column Compartments





Choose from binary, quaternary, dual-gradient, isocratic and nano/capillary/micro HPLC and UHPLC pumps for reproducible separations. Achieve accurate injection across the entire injection range for fast, reproducible sample injection with minimum carryover. Benefit from constant column temperature control for stable retention times. Quantify your target analytes with a choice of robust and sensitive HPLC and UHPLC detectors.



Build your ideal HPLC or UHPLC with our step-by-step configurator


Experience industry-leading operating pressure, flow accuracy, and flow precision when you use Thermo Scientific Vanquish pumps. Vanquish HPLC and UHPLC pumps provide optimized gradient delay volume, exceptional gradient profiles, unmatched flow accuracy, and durability. All Vanquish pumps are built with Thermo Scientific SmartFlow technology to automatically compensate for changing solvent compressibility.

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HPLC and UHPLC pump categories

Binary pumps

High throughput for high-resolution HPLC, UHPLC or LC-MS applications.

Quaternary pumps

Wide application range and flexibility in mobile phase composition.

Dual-gradient pumps

Increased productivity, flexibility and switching between applications without re-plumbing.

Isocratic pumps

Stable peak retention times for routine isocratic applications.

Nano/capillary/micro pumps

Accurate gradients and flow precision from nano- to micro-flow for maximized sensitivity and robustness.


Innovative and carefully designed Thermo Scientific HPLC and UHPLC autosamplers and sampler extension modules ensure reliable, precise and accurate injection within their operating range. They support a wide range of sample rack formats.

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Column Compartments

Control your separations with more confidence using Thermo Scientific HPLC and UHPLC column compartments. Active or passive preheating ensure that the solvent entering the column matches the column temperature and reduces performance loss from temperature effects. Two column thermostatting modes – still and forced air – allow you to refine your separation to easily match your existing methods and ensure even temperatures for UHPLC analysis.

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We offer a wide array of detectors for the Thermo Scientific Vanquish HPLC and UHPLC systems to confidently detect the analytes in your sample.

Discover HPLC and UHPLC Detectors

HPLC and UHPLC detector options

Charged Aerosol Detector (CAD)

Delivers sensitive universal detection and near-uniform response for excellent quantification of targeted analytes.

Diode Array Detector (DAD)

Offers the highest performance and operating range for detecting UV-Vis spectra.

Fluorescence Detector (FLD)

Provides high detection sensitivity and multiple detection options for effective method development.

Multiple Wavelength Detector (MWD)

Provides sensitive monitoring of several UV-Vis traces simultaneously.

Variable Wavelength Detector (VWD)

Combines low noise, drift and linearity performance to support a wide operating range and trace compound detection.

Mass Spectrometer (MS)

Provides maximum sensitivity and selectivity for robust and reliable detection, even for challenging nano-flow-rate gradients.

Refractive Index Detector (RID)

Offers a cost-effective solution to support the analysis of sugars, polymers, and other compounds that do not contain a chromophore.

pH and Conductivity Monitor

Provides accurate online pH and conductivity measurements in combination with DAD, MWD, or VWD.

Tubing, Kits, and Accessories

Thermo Scientific Viper and nanoViper Fingertight Fittings, tubing, kits, and accessories allow you to quickly connect HPLC modules, valves, and columns. They enable leak-free connections that improve chromatographic results by minimizing dead volume.  
Viper and nanoViper fittings require no tools for installation, fit narrow connections (e.g., 10-port valves), and combine simplicity with high performance.

Discover HPLC and UHPLC Tubings, Kits, and Accessories

What makes Viper fittings better than conventional fittings?

Thermo Scientific Viper Fingertight fittings, for HPLC and UHPLC systems, seal at the tip of the tubing and virtually eliminate dead volume. These reusable fittings make it easier to plumb your HPLC or UHPLC system without any tools for reproducible leak-free connections to pressures up to 1500 bar.

Conventional fittings

Conventional fittings often create extra-column volumes by incorrect positioning of the ferrule or by the capillary slipping through the ferrule when subjected to high pressures. The chromatogram demonstrates deteriorated peak shape caused by a slipped capillary at a backpressure of only 600 bar (8700 psi).

Viper fingertight fittings

Viper fingertight fittings does not use a ferrule and minimizes extra-column volume by design. The chromatogram overlay shows consistent peak shapes under identical conditions to those used with conventional fitting in the figure above.

Optional Components

Vanquish Solvent Monitor


Select HPLC and UHPLC systems are compatible with the Vanquish Solvent Monitor, which accurately measures the liquid levels in each solvent channel and the waste container to streamline your Standard Operating Procedures (SOPs). As part of the sequence ready check, the system automatically warns you if there is not enough eluent or waste capacity to complete the sample sequence, preventing system and column damage and ensuring ‘right the first time’ results.  

Vanquish User Interface


View system health, performance, and equilibration status at a glance with the Thermo Scientific Vanquish User Interface. This user-friendly interface enables operators to quickly understand system status and solve problems. The multi-language interface comes with simple maintenance tutorials to guide even novice operators in keeping the system reliably delivering results.

Fraction Collectors


Thermo Scientific fraction collectors support purification from nano- to semipreparative applications. The high-performance Thermo Scientific Vanquish Fraction Collector fully integrates with our Thermo Scientific Vanquish Analytical Purification LC Systems, allowing you to achieve pinpoint purification by fractionation with precision.

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