Vanquish Fluorescence Detectors (FLD)

For compounds that exhibit fluorescence

Fluorescence detectors rely on the ability of some compounds to emit light after activation with light of a different wavelength. Many pharmaceuticals, natural products, petroleum products, and biomolecules fluoresce naturally; other compounds, such as amino acids, proteins, or glycans can be labeled with fluorescent tags. With careful wavelength selection and filtering of excitation and emission light, users can enable fluorescence detectors to reach high selectivity, along with 10 to 1,000 times more sensitivity than UV detectors.

The optical design of the Thermo Scientific Vanquish Fluorescence Detectors provide you with the best detection sensitivity and superior signal-to-noise performance through highly effective stray light suppression. Thermal effects are reduced with temperature-controlled flow cells for increased detection precision.

Monitor up to four excitation/emission wavelength pairs simultaneously in multichannel operation mode or scan your chromatogram for the best emission/excitation wavelengths. Improve detection sensitivity and selectivity by ultrafast wavelength switching between peaks. Vanquish Fluorescence Detectors are easy to use and provide you with an unmatched detection experience.

  • Acquire data at up to 200 Hz for support of even the fastest UHPLC separations
  • Optimize your sensitivity through xenon flash lamp frequency and variable emission filter settings
  • Simplify method development or improve existing methods using single spectrum scans or fluorescence field acquisition in excitation, emission, or synchronous mode
  • Reduce your cost of ownership through increased lamp lifetime from the long-life xenon flash lamp and various lamp operation modes
  Fluorescence
Detector F
Fluorescence
Detector F
with Dual-PMT
Fluorescence
Detector C
Fluorescence
Detector C
with Dual-PMT
Excitation wavelength range (Emission wavelength range) Great Excitation Wavelength Range
200 – 630 nm
(220 – 650 nm)
Excellent Excitation Wavelength Range
200 – 880 nm
(220 – 900 nm)
Great Excitation Wavelength Range
200 – 630 nm
(220 – 650 nm)
Excellent Excitation Wavelength Range
200 – 880 nm
(220 – 900 nm)
Maximum number of signal channels Excellent Maximum Number of Signal Channels
4
Excellent Maximum Number of Signal Channels
4
Good Maximum Number of Signal Channels
1
Good Maximum Number of Signal Channels
1
Data collection rate Excellent Data Collection Rate
Up to 200 Hz
Excellent Data Collection Rate
Up to 200 Hz
Great Data Collection Rate
Up to 100 Hz
Great Data Collection Rate
Up to 100 Hz
Emission Filter Excellent Emission Filter
Variable: 5 positions
Excellent Emission Filter
Variable: 5 positions
Good Emission Filter
Fixed: 280 nm
Good Emission Filter
Fixed: 280 nm

Develop highly selective methods for trace analytes or novel molecules with sensitivity three to six orders of magnitude greater than UV detection. The Thermo Scientific Dionex UltiMate 3000 Fluorescence Detectors offer four channel acquisition at up to 200Hz so you can optimize quantification on the most sensitive channel and single-channel detection at up to 100Hz. 2D or 3D synchro, excitation, emission scans, zero order mode, or multichannel acquisition offer flexibility for method development or routine sample characterization. A variable emission filter allows real-time, compound-related sensitivity optimization.

FLD-3100 Fluorescence Detector
  • Economical choice for laboratories on a limited budget, offering single-wavelength detection at up to 100 Hz.
  • Excitation wavelength: 200 to 630 nm
  • Emission wavelength: 265 to 650 nm
FLD-3100 Fluorescence Detector with Dual-PMT
  • Extended wavelength range choice for laboratories on a limited budget, offering single-wavelength detection at up to 100 Hz.
  • Excitation wavelength: 200 to 880 nm
  • Emission wavelength: 265 to 900 nm
FLD-3400 RS Rapid Separation Fluorescence Detector
  • Employing a 200 Hz data collection rate to capture all the information from narrow RS peaks, with up to four wavelengths monitored simultaneously.
  • Excitation wavelength: 200 to 880 nm
  • Emission wavelength: 220 to 650 nm
FLD-3400 RS Rapid Separation Fluorescence Detector with Dual-PMT
  • Extended wavelength choice for laboratories performing Rapid Separation, with a 200 Hz data collection rate to capture all the information from narrow RS peaks, and up to four wavelengths monitored simultaneously.
  • Excitation wavelength: 200 to 880 nm
  • Emission wavelength: 220 to 900 nm

The optical design of the Thermo Scientific Vanquish Fluorescence Detectors provide you with the best detection sensitivity and superior signal-to-noise performance through highly effective stray light suppression. Thermal effects are reduced with temperature-controlled flow cells for increased detection precision.

Monitor up to four excitation/emission wavelength pairs simultaneously in multichannel operation mode or scan your chromatogram for the best emission/excitation wavelengths. Improve detection sensitivity and selectivity by ultrafast wavelength switching between peaks. Vanquish Fluorescence Detectors are easy to use and provide you with an unmatched detection experience.

  • Acquire data at up to 200 Hz for support of even the fastest UHPLC separations
  • Optimize your sensitivity through xenon flash lamp frequency and variable emission filter settings
  • Simplify method development or improve existing methods using single spectrum scans or fluorescence field acquisition in excitation, emission, or synchronous mode
  • Reduce your cost of ownership through increased lamp lifetime from the long-life xenon flash lamp and various lamp operation modes
  Fluorescence
Detector F
Fluorescence
Detector F
with Dual-PMT
Fluorescence
Detector C
Fluorescence
Detector C
with Dual-PMT
Excitation wavelength range (Emission wavelength range) Great Excitation Wavelength Range
200 – 630 nm
(220 – 650 nm)
Excellent Excitation Wavelength Range
200 – 880 nm
(220 – 900 nm)
Great Excitation Wavelength Range
200 – 630 nm
(220 – 650 nm)
Excellent Excitation Wavelength Range
200 – 880 nm
(220 – 900 nm)
Maximum number of signal channels Excellent Maximum Number of Signal Channels
4
Excellent Maximum Number of Signal Channels
4
Good Maximum Number of Signal Channels
1
Good Maximum Number of Signal Channels
1
Data collection rate Excellent Data Collection Rate
Up to 200 Hz
Excellent Data Collection Rate
Up to 200 Hz
Great Data Collection Rate
Up to 100 Hz
Great Data Collection Rate
Up to 100 Hz
Emission Filter Excellent Emission Filter
Variable: 5 positions
Excellent Emission Filter
Variable: 5 positions
Good Emission Filter
Fixed: 280 nm
Good Emission Filter
Fixed: 280 nm

Develop highly selective methods for trace analytes or novel molecules with sensitivity three to six orders of magnitude greater than UV detection. The Thermo Scientific Dionex UltiMate 3000 Fluorescence Detectors offer four channel acquisition at up to 200Hz so you can optimize quantification on the most sensitive channel and single-channel detection at up to 100Hz. 2D or 3D synchro, excitation, emission scans, zero order mode, or multichannel acquisition offer flexibility for method development or routine sample characterization. A variable emission filter allows real-time, compound-related sensitivity optimization.

FLD-3100 Fluorescence Detector
  • Economical choice for laboratories on a limited budget, offering single-wavelength detection at up to 100 Hz.
  • Excitation wavelength: 200 to 630 nm
  • Emission wavelength: 265 to 650 nm
FLD-3100 Fluorescence Detector with Dual-PMT
  • Extended wavelength range choice for laboratories on a limited budget, offering single-wavelength detection at up to 100 Hz.
  • Excitation wavelength: 200 to 880 nm
  • Emission wavelength: 265 to 900 nm
FLD-3400 RS Rapid Separation Fluorescence Detector
  • Employing a 200 Hz data collection rate to capture all the information from narrow RS peaks, with up to four wavelengths monitored simultaneously.
  • Excitation wavelength: 200 to 880 nm
  • Emission wavelength: 220 to 650 nm
FLD-3400 RS Rapid Separation Fluorescence Detector with Dual-PMT
  • Extended wavelength choice for laboratories performing Rapid Separation, with a 200 Hz data collection rate to capture all the information from narrow RS peaks, and up to four wavelengths monitored simultaneously.
  • Excitation wavelength: 200 to 880 nm
  • Emission wavelength: 220 to 900 nm