Nano, Capillary, and Microflow UHPLC Systems

Ideal for low-flow LC-MS workflows

Do you have applications requiring high sensitivity such as proteomics, metabolomics, and intact protein analysis? The Thermo Scientific EASY-nLC 1200 system provides effortless nanoflow UHPLC, and the Thermo Scientific UltiMate 3000 RSLCnano system provides fully integrated and versatile nano-, capillary-, and micro-flow solutions.

Browse our complete portfolio below, or let one of our experts help you tailor a selection specific to your application and workflow needs.

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The right solution for every low-flow application

Experience outstanding performance and workflow versatility with the Thermo Scientific UltiMate 3000 RSLCnano and Thermo Scientific EASY-nLC 1200 systems. These low-flow UHPLC systems feature a wide flow-pressure footprint and are ready for nanoLC-MS with long columns, enabling deep dive proteomics experiments as well as high-throughput LC-MS analysis of large sample cohorts. The UltiMate 3000 RSLCnano and EASY-nLC 1200 systems offer excellent retention time precision, improved flow rate control, and intelligent operation so that you achieve greater confidence in your results.

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Choose from two low-flow UHPLC systems

A compact, integrated, and intuitive nanoLC system that offers effortless ultra-high performance for everyone. The EASY-nLC 1200 can handle ultra-long columns due to its high-pressure capability for maximum resolution of complex mixtures.

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This versatile and customizable low-flow UHPLC platform offers flexibility for routine and advanced LCMS workflows. The UltiMate 3000 RSLCnano system offers excellent retention time precision and gradient accuracy for nano-, capillary, and micro-flow rates for more confidence in your LCMS results.

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Low-flow UHPLC systems comparison

    EASY-nLC 1200

EASY-nLC 1200

Operational simplicity and excellent performance
UltiMate 3000 RSLCnano

UltiMate 3000 RSLCnano

Versatility and uncompromising precision


System pressure
Retention time precision
Splitless nano-flow    
Nano-flow range Recommended range:
100 nL/min–1.0 μL/min
Recommended range:
50 nL/min–1.5 μL/min
Capillary-flow range Recommended range:
0.5–10 μL/min
Micro-flow range Recommended range:
5–50 μL/min

System Features

Design Integrated Modular
Column compartment n.a.*
*Built-in heating with
EASY-Spray columns
Sample capacity
Sample cooling    
nanoViper fittings    
Zero-loss sample pick-up    
Nano-flow pump High-pressure mixing binary syringe pump High-pressure mixing binary continuous flow pump
Analytical flow and loading pump Low-pressure mixing ternary gradient pump

Application Setups

Direct injection    
Preconcentration Vented column setup Continuous direct flow by integrated loading pump
2D salt setups  
Automated online 2D LC*  
Offline fractionation*  
Tandem LC*  
*requires additional equipment


Software Xcalibur, LC Devices,
& Tracefinder
SII with Xcalibur & Tracefinder
Chromeleon Chromatography
Data System
Integrated PC  
Audit trail  


MS sources for MS integration EASY-Spray Source
Nanospray Flex Ion Source
EASY-Spray Source
Nanospray Flex Ion Source
Ion Max Ion Source
Columns EASY-Spray C18 Columns
Integrated column/emitter design minimizing dead volume.

Acclaim PepMap 100 C18 Columns
Acclaim PepMap 300 C18 Columns
Columns ideal for peptide and protein separations with inner
diameters between 50 μm and 1 mm

Any of our HPLC and UHPLC column
= Not applicable = Poor = Good = Better = Best

Thermo Scientific Viper and nanoViper HPLC fittings, connectors, tubing, and kits allow you to quickly connect HPLC modules, valves, and columns for leak-free connections that improve chromatographic results by minimizing dead volume. Viper and nanoViper fittings systems and kits require no tools for installation, fit narrow connections (e.g., 10-port valves), and combine simplicity with high performance.

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Featured webinar
Raise your nano LC-MS performance with new Thermo Scientific solutions

In this webinar, scientists discuss the latest innovations in nano LC-MS technology with overviews of the EASY-nLC 1200 and versatile UltiMate 3000 RSLCnano systems.

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