For researchers pursuing the next scientific breakthrough, the Thermo Scientific Vanquish Neo UHPLC system delivers maximum performance 24/7 for reproducible nano-, capillary-, and micro-flow LC-MS. Combining a high degree of innovation with the best-in-class Vanquish platform enhances productivity for novice and expert LC-MS users alike.

Build your ideal HPLC or UHPLC with our step-by-step configurator


All-in-one nano-, capillary-, and micro-flow LC

Thermo Scientific ProFlow™ XR active flow control pump technology delivers excellent retention time precision from nano up to 100 μL/min at pressures up to 1500 bar. Vial bottom detection enables injections from volume-limited samples.

Accelerated productivity

Fast sample loading, column equilibration and ultra-low system gradient delay volume reduce method overhead time, improving sample throughput while enhancing MS utilization.

Maximized performance

Unique low-flow split-loop autosampler design limits sample dispersion, reduces system carryover, and maximizes sample usage. The metering device improves quantitation through high injection volume linearity, precision, and accuracy.

Long-term, trouble-free analysis

Next-generation Thermo Scientific SmartInject technology improves column lifetime and retention time precision. Maintenance-free 1500 bar compatible ceramic valves and near-zero dead volume Thermo Scientific Viper and nanoViper fittings minimize leaks with robust long-term operation.

Intelligent operation

The Thermo Scientific Vanquish User Interface provides walk-up and remote system monitoring and control with automated procedures and guided instrument maintenance and troubleshooting scripts, improving productivity for all users. The intelligent method editor wizard ensures compatibility of method parameters with installed consumables.

Seamless LC-MS integration

The Vanquish Neo UHPLC system excels at high-sensitivity LC-MS applications and seamlessly integrates with the Thermo Scientific mass spectrometry portfolio through a comprehensive suite of ESI sources. Harness speed, robustness and sensitivity with triple quadrupole MS systems or resolution, mass accuracy, and versatility with Thermo Scientific Orbitrap MS systems.


Explore a new dimension in proteomics quantitation

System specifications

The table below provides an overview of the Vanquish Neo UHPLC system specifications for a standard set up. Not all specifications are listed. For additional information, download the Vanquish Neo UHPLC System specifications sheet.


Vanquish Neo UHPLC System

System pressure, maximum

1500 bar

Flow range

1 nL/min – 100 μL/min, in 1 nL increments 

(100 nL/min – 100 μL/min, recommended)

Pump options (default gradient delay volume)

Binary (< 25 nL)



pH range

2 – 10 

System design

Integrated, with slide-in modules

Touchscreen user interface display compatible


Active flow control for maximum retention time precision

Yes, Thermo Scientific ProFlow XR pump technology

SmartInject technology for maximum injection precision and improved column lifetime


Injection volume range

Standard: 0.01–25 μL
Optional: 0.01–100 μL
High volume trapping by multiple draw-and-trap cycles up to 500 μL 

Sample cooling

Yes, 4 – 40°C 

Sample capacity (standard)

Based on standard 12 mm OD vials (≤ 1.5 mL)

4 racks of 54 vials (≤ 1.5 mL) each, other rack formats available 
+ capacity of 12 × 22.5 mm OD vials (≤ 10 mL) in the carousel

Sampler extension available (optional)


Column compartment thermostatting

Yes (optional) 
Minimum 5 K above room temperature to 80 ⁰C
Forced air

Column compartment pre-heater

Passive (optional)

Column compartment post-column cooler


Detector choices

Orbitrap mass spectrometers
Triple-quadrupole mass spectrometers
Variable wavelength detector F

Optional components: Fraction Collector Visit our Vanquish Fraction Collector FT page

Contact us to get a quote or discuss an HPLC/UHPLC solution

For Research Use Only. Not for use in diagnostic procedures.