Vanquish Simple Switch 2D-LC Systems

Two independent flow paths for more confidence and productivity.

Vanquish Simple Switch 2D-LC Systems

Seamlessly switch between a trap heart-cut 2D-LC system and a dual LC system using the innovative Thermo Scientific Vanquish Simple Switch 2D-LC System. This two-dimensional LC (LC-LC) system is an innovative, all-in-one system that uses the novel Thermo Scientific Vanquish Dual Split Sampler to enable users to seamlessly switch between both system capabilities without re-plumbing. Simply decide which task needs to be performed and this system supplies the confidence and productivity you need.  


Two systems in one

The unique system configuration allows you to run both trap-based 2D-LC for difficult samples and switch to a dual LC system that uses two independent 1D flow paths to run two analyses, either identical or not, on a single system without re-plumbing.

Greater confidence for complex sample analysis

Run sensitive LC-LC methods by enriching the fractionated analytes on a trap column to enable low-concentration detection and prevent solvent incompatibility between detection methods; or run two complementary assays simultaneously on two separate flow paths to double throughput.

Increased sensitivity

The system enables enrichment of low-abundant analytes and removal of diluting or disturbing solvents from the eluent by trapping and pre-concentrating the analytes of interest on a trap column to increase sensitivity when used as a 2D-LC instrument.

Simplified method development

The second-dimension pump provides the flow for solvent modulation, to address solvent incompatibility between UV and MS methods or mobile phase incompatibility between different column chemistries to support multiple combinations of 1D and 2D methods.


The modularity of the Vanquish LC systems makes it easy to build a system to best meet your application requirements with the flexibility to upgrade from a standard HPLC instrument to a more advanced configuration when you need to address more challenging analyses.  

Easy and reliable tool-free connections

The Vanquish Simple Switch 2D-LC system is fitted with Thermo Scientific Viper fingertight fittings, which support near-zero dead volume connections and enable sharper peaks and tool-free setup.

What is the Vanquish Simple Switch 2D-LC System?

Our unique Vanquish Simple Switch 2D-LC system allows you to simply switch between a trap-based 2D-LC or dual LC.


This flow scheme illustrates how the three six-port valves can be configured to run the system for 2D-LC analysis with two columns with a trap column in series, or as a dual LC system with two columns in parallel. 

All-in-one system: LC-LC system with trap and solvent modulation (left) OR Dual LC system with two independent ¹D flow path in one system (right).


System specifications

The table below provides an overview of the Vanquish Simple Switch 2D-LC system specifications for standard setups. Specifications may differ based on the specific system components selected. See detailed Component Specifications for more information.

Category Vanquish Simple Switch 2D-LC System
System pressure, maximum 1000 or 1500 bar, depends on system configuration
Flow range 0–5 or 0-8 mL/min, depends on pumps selected
Pump options (default gradient delay volume)

Binary (35, 200µL) 
Quaternary (679 µL) 

Delay volume depends on pump(s) selected

Biocompatible Yes
pH range 2–12, chloride concentration up to 1 mol/L
System design Modular
Touchscreen user interface display compatible (optional) Yes, depends on system configuration
Solvent monitoring system compatible (optional) Yes, depends on system configuration
Customizable gradient delay volume to simplify method transfer Yes, with metering device
SmartInject technology for maximum injection precision and improved column lifetime Yes
Injection volume range

Standard: 0.01-25 µL  
Optional: 0.01-100 µL

Sample cooling Yes, 4-40°C

Sample capacity (standard) 
Based on 12 mm OD vials (≤1.5 mL) for comparison

4 racks of 54 vials (≤ 1.5 mL) each, other rack formats available 
+ capacity of 12 × 22.5 mm OD vials (≤ 10 mL) in the carousel

Sample extension available (optional) Yes, with Vanquish Charger
Column compartment thermostatting

Yes, 5-120°C 
Still air and forced air

Column compartment pre-heater

Active and passive 

Column compartment post-column cooler Yes
Compatible Detectors

Vanquish Charged Aerosol Detector 

Vanquish Diode Array Detector  

Vanquish Fluorescence Detector 

Vanquish Multiple Wavelength Detector 

Vanquish Variable Wavelength Detector

Refractive Index Detector 

Mass Spectrometer

Component specifications

Standard Vanquish Simple Switch 2D-LC systems are made up of the following components. Download the associated specifications sheet for more detail on each component. 

  Vanquish Horizon Simple Switch 2D-LC System

Vanquish Flex Simple Switch 2D-LC System



Download Specification Sheet

Vanquish Binary Pump H 
Vanquish Binary Pump H 
Vanquish Quaternary Pump F

Vanquish Binary Pump F 
Vanquish Binary Pump F 
Vanquish Quaternary Pump F 

Note: Binary pump recommended as ²D pump



Download Specification Sheet

Vanquish Split Sampler HT Vanquish Split Sampler FT

Extended sample capacity (optional)


Download Specification Sheet

Vanquish Charger  

Column compartment


Download Specification Sheet

Vanquish Column Compartment H 

Virtually zero dead volume system connections


Download Specification Sheet

Viper UHPLC Fittings



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For Research Use Only. Not for use in diagnostic procedures.