Vanquish UHPLC Systems

Separate your science from the status quo

Thermo Scientific Vanquish UHPLC systems are the most advanced UHPLC instruments available. The new Vanquish platform was designed from the ground up to improve performance and repeatability with no trade-offs in quality, robustness, or ease of use. Vanquish platform users have all they need to solve their toughest analytical challenges with confidence.

Advanced UHPLC systems for solving tough analytical challenges

For any application—whether HPLC or UHPLC, small or large molecules, simple or complex mixtures—the Vanquish platform was designed to handle it all. This innovative, simple-to-operate, and easy-to-maintain platform delivers confident separations that push chromatographic boundaries. Regardless of the configuration you choose, you will get a highly integrated solution with optimal fluidic connections, providing unsurpassed retention time and peak area precision.

You will also benefit from the industry-leading, easy-to-use Thermo Scientific Chromeleon Chromatography Data System (CDS) software with eWorkflow method setup, dedicated ePanels, and smart tools. Chromeleon CDS software delivers advanced system communication with single-point intelligent control and functionality. It also provides direct plugins for the Vanquish system to other manufacturers' chromatography data systems.

Choose from three Vanquish UHPLC systems:

For your most complex and difficult samples, or for applications where you are aiming for the shortest cycle times and highest throughput. Vanquish Horizon allows for smaller particles and column dimensions to be run with greater confidence for the most demanding LC and LC-MS workflows.

 Vanquish Horizon UHPLC brochure

For assays that require more flexibility in solvents to accommodate all applications. Vanquish Flex excels from method development to routine analyses. With binary and quaternary pump options, the Vanquish Flex offers outstanding performance for targeted LC and LC-MS workflows.

 Vanquish Flex UHPLC brochure

Compare Vanquish UHPLC systems

Vanquish Horizon
UHPLC System

Vanquish Flex
Binary UHPLC System

Vanquish Flex
Quaternary UHPLC System

    Most advanced UHPLC with revolutionary flow delivery High pressure binary solvent blending for fast, reliable UHPLC Quaternary solvent blending for flexible method development


System pressure
Up to 1500 bar

Up to 1000 bar

Up to 1000 bar
Retention time precision
Flow range
5 mL/min

8 mL/min

8 mL/min

System Features

Design Modular Modular Modular
SmartInject technology for best retention time precision      
SmartFlow technology for excellent flow precision      
Pump High-pressure gradient proportioning High-pressure gradient proportioning Low-pressure gradient formation
Active solvent
Multi-mode column thermostatting      
Sample cooling      
Viper fittings for virtually zero dead volume system connections      
    Thermo Scientific Viper UHPLC Fittings Kits
Order all the UHPLC fittings and capillaries you need for your system or application in a single kit

System components

Pump Vanquish Binary Pump H
The pump with the highest backpressure at 1500 bar; ideal for shallow gradient performance
Vanquish Binary Pump F
UHPLC performance for demanding routine applications up to 1000 bar
Vanquish Quaternary Pump F
UHPLC performance with more solvent flexibility up to 1000 bar
Autosampler Vanquish Split Sampler HT
Excellent precision, accuracy, and short cycle times with innovative air-stream cooling and barcode reading for inventory management for all workflows up to 1500 bar
Vanquish Split Sampler FT
Enjoy the same benefits as the Vanquish Split Sampler HT for applications up to 1000 bar
Column compartment Vanquish Column Compartment H
Provides two column thermostatting modes (still and forced air), active and passive solvent pre-heating and post column cooling for optimized control of your separations and advanced method transfer.
Extended sample capacity Vanquish Charger
An intelligent, robotic rack and plate feeder that automatically loads samples in the autosampler and extends the sample capacity of all Vanquish samplers to up to 8832 samples (23 wellplates or 12 vial racks) with innovative air-stream cooling and barcode reading for inventory management.
= Not applicable = Poor = Good = Better = Best

Compatible components for all Vanquish UHPLC systems

Detector options Vanquish columns Chromeleon CDS software

Take advantage of the system's extended pressure capabilities and robustness by using columns that were developed for Vanquish, allowing you to separate your science better, faster, and easier than ever before.

Vanquish can be used with any of our HPLC and UHPLC columns

  • Streamlines your data processing
  • Industry-leading ease of use and intelligent functionality
  • Unique user guidance and hardware automation features designed for Vanquish systems

Not sure which system is right for you?

Connect with an expert to help you decide

Contact us

Interact with Vanquish

Take a virtual tour of the Vanquish Horizon, and see how each module's advanced technology will separate your science from the status quo.

Take virtual tour ›

Featured Vanquish webinar

Dr. Frank Steiner and Dr. James Grinias present new features in the Vanquish system that allow for easy method transfer, and discuss how thermal column environments can revolutionize your UHPLC experience. Discover how you can push chromatographic boundaries to separate your science from the status quo.

 Register to watch the webinar

Featured Vanquish UHPLC system videos