Vanquish UHPLC Systems

Separate your science from the status quo

Thermo Scientific Vanquish UHPLC systems are the most advanced UHPLC instruments available. The new Vanquish platform was designed from the ground up to improve performance and repeatability with no trade-offs in quality, robustness, or ease-of-use. Vanquish platform users have all they need to solve their toughest analytical challenges with confidence.

Advanced UHPLC systems for solving tough analytical challenges

For any application—whether HPLC or UHPLC, small or large molecules, simple or complex mixtures—the Vanquish platform was designed to handle it all. This innovative, simple-to-operate, and easy-to-maintain platform delivers confident separations that push chromatographic boundaries. Regardless of the configuration you choose, you will get a highly integrated solution with optimal fluidic connections, providing unsurpassed retention time and peak area precision.

You will also benefit from the industry-leading, easy-to-use, and compliant Thermo Scientific Chromeleon Chromatography Data System (CDS) with eWorkflow method setup, dedicated ePanels, Open Access, and smart tools. Chromeleon CDS delivers advanced system communication with single-point intelligent control and functionality. It also provides direct plugins for the Vanquish system to other manufacturers' chromatography data systems.

Choose from three Vanquish UHPLC systems:

For your most complex and difficult samples, or for applications where you are aiming for the shortest cycle times and highest throughput. Vanquish Horizon UHPLC system allows for smaller particles and column dimensions to be run with greater confidence for the most demanding LC and LC-MS workflows.

For assays that require more flexibility in solvents to accommodate all applications. Vanquish Flex UHPLC systems excel from method development to routine analyses. With binary and quaternary pump options, the Vanquish Flex UHPLC systems offer outstanding performance for targeted LC and LC-MS workflows.

Increase productivity, up-time, and sample knowledge for a faster return on investment. Vanquish Duo UHPLC systems expands the benefits of the Vanquish platform through two flow paths to support three workflows (dual, tandem and inverse gradient) within one integrated UHPLC system. With the addition of the second flow path, improve your productivity by saving time, cost per sample without added bench space, and without compromising performance, robustness or ease-of-use.

Compare Vanquish UHPLC systems

Vanquish Horizon
UHPLC System

Vanquish Flex
UHPLC Systems

Vanquish Duo
UHPLC Systems

    Unrivaled performance and throughput for applications requiring high-end UHPLC Binary or quaternary solvent blending for fast and reliable UHPLC or flexible method development Increase productivity with two flow paths to support three workflows


System pressure
Up to 1500 bar

Up to 1000 bar

Up to 1500 bar*
Retention time precision
Flow range
5 mL/min

8 mL/min

8 mL/min*
Design Modular

System components

Pump Vanquish Binary Pump H
The pump with the highest backpressure at 1500 bar, and ideal for any gradient performance from shallow to ballistic
Vanquish Binary Pump F
UHPLC performance for demanding routine applications up to 1000 bar

Vanquish Quaternary Pump F
UHPLC performance with more solvent flexibility up to 1000 bar
Vanquish Dual Pump F
Uniquely provides two independent ternary-solvent blending flow streams in one device up to 1000 bar

Instrument can also be configured with a combination of Vanquish Horizon or Vanquish Flex pumps
Autosampler Vanquish Split Sampler HT
Excellent precision, accuracy, and short cycle times with innovative air-stream cooling and barcode reading for inventory management for all workflows up to 1500 bar
Vanquish Split Sampler FT
Enjoy the same benefits as the Vanquish Split Sampler HT for applications up to 1000 bar
Vanquish Dual Split Sampler FT/HT
Unique sampler that combines two injection devices in one housing resulting in two separate channels that are individually controllable (Inj. volume, wash cycles, etc.) with a 1000 bar or 1500 bar option

Instrument can also be configured with a single Vanquish Split Sampler HT/FT
Extended sample capacity Vanquish Charger
An intelligent, robotic rack and plate feeder that automatically loads samples in the autosampler and extends the sample capacity of all Vanquish samplers to up to 8832 samples (23 wellplates or 12 vial racks) with innovative air-stream cooling and barcode reading for inventory management
Column compartment Vanquish Column Compartment
Provides two column thermostatting modes (still and forced air), active and passive solvent pre-heating and post column cooling for optimized control of your separations and advanced method transfer
Virtually zero dead volume system connections Viper UHPLC Fittings Kits
Order all the UHPLC fittings and capillaries you need for your system or application in a single kit
= Not applicable = Poor = Good = Better = Best

* Different combinations of Vanquish pumps or autosamplers can be used for desired configuration

Compatible components for all Vanquish UHPLC systems

Detector options Vanquish columns Chromeleon CDS
Extend pressure capabilities and robustness by using columns that were developed for Vanquish systems, allowing you to separate your science better, faster, and easier than ever before.
All Vanquish systems can be used with any of our HPLC and UHPLC columns
  • Streamlines your data processing
  • Industry-leading ease of use and intelligent functionalities
  • Outstanding data integrity
  • Unique user guidance and hardware automation features designed for Vanquish systems

Featured Vanquish webinar

Dr. Frank Steiner and Dr. James Grinias present new features in the Vanquish platform that allow for easy method transfer, and discuss how thermal column environments can revolutionize your UHPLC experience. Discover how you can push chromatographic boundaries to separate your science from the status quo.

 Register to watch the webinar

Featured Vanquish UHPLC system videos