HPLC and UHPLC for Biologics Development, Identification & QC

Now you just need one system

Drug development workflows in biopharmaceutical laboratories can be demanding, requiring extensive data, accurate results, and fast separations. The Dionex UltiMate 3000 Biocompatible Rapid Separation (BioRS) system perfectly matches the demands for biomolecule separations to run various modes of chromatography in UHPLC. This goes beyond reversed-phase UHPLC: it is genuine Bio UHPLC for proteins, peptides, and biotherapeutics.

BioRS system features

Selection of main features:

  • Pressure of up to 100MPa (15,000 psi)
  • Flow rates of up to 8mL/min
  • Short sampler cycle times (< 20 seconds)
  • Wide column thermostatting range (5°C to 110°C)
  • Ultrafast data collection and processing (up to 200Hz)

The system was developed for reliability and robustness under the harsh solvent conditions applied in biochromatographic analyses. Corrosion-resistant materials in the complete system flow path reduce the risk of analyte interaction with internal surfaces and extend the lifetime of particular biocolumns, even under high salt or extreme pH conditions.

The UltiMate 3000 BioRS system is a modular platform that offers several options for each component. All are fully biocompatible and UHPLC capable. This means that the UltiMate 3000 BioRS can be configured for any bioUHPLC analysis to deliver optimal separations at ultrahigh speed while maintaining resolution.

Unique Column Chemistries
Unique column chemistries

The gold standard ProPac WCX and MAbPac columns can be used with salt or pH gradient LC. In addition new chemistries like the GlycanPac AXH-1 column are available to separate both labeled and unlabeled N- and O-glycans by charge, size, and isomer.

Seamless MS Integration
Seamless MS integration

The UltiMate 3000 BioRS system combines easily with our best-in-class mass spectrometers. Routine confirmation on iontrap or triple quad mass spectrometers, or discovery on high resolution Q Exactive, Exactive Plus or Orbitrap hybrids, all seamlessly integrate with the UHPLC+ solutions offered by the UltiMate 3000 BioRS system. 

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