More than 50% of all active pharmaceutical substances (APIs) are administered as salts. The different ions are used to alter the biological and physicochemical properties such as solubility, stability, and dissolution rate. Thus, the screening and the assaying of a broad variety of counterions and APIs is crucial. To obtain such information a variety of traditional techniques such as potentiometric titration and ion chromatography (IC) with conductivity detection already exist. These techniques cannot, in contrast to the IonCount solution, simultaneously analyze several cations and anions or analyze the API simultaneously with its respective counterion.

The Thermo Scientific™ IonCount Solution is a solution for the analysis of a wide range of pharmaceutically-relevant counterions and APIs. The solution is based on a combination of a dedicated column technology and the unique charged aerosol detection. IonCount includes a complete Thermo Scientific™ Dionex™ UltiMate™ 3000 Standard system and a chromatography starter kit. This kit includes two different analytical columns providing alternative selectivity, all required buffer salts for the mobile phases as well as a reference test mix. IonCount uses Thermo Scientific™ Dionex™ Chromeleon™ 7.2 Chromatography Data System eWorkflow for an easy and fast sequence setup. Pre-defined eWorkflows in combination with the reference test mix allow for a continuously monitoring system and column performance, ensuring high-quality data. Additional, pre-defined eWorkflows make the assaying of a wide range of cationic and anionic counterions as well as APIs as easy as ever before.

Downloads

 Brochure: IonCount Solution: Complete Solution for Pharmaceutical Counterions

 Application Note 1047: Simultaneous Determination of Tartaric Acid and Tolerodine in Tolterodine Tartrate

 Application Note 20868: Simultaneous Determination of Metformin and its Chloride Counterion Using Multi-Mode Liquid Chromatography with Charged Aerosol Detection

 Application Note 20870: API and Counterions in Adderall Using Multi-mode Liquid Chromatography with Charged Aerosol Detection

 Application Note 20871: Separation of Calcium, Magnesium and Counterions in a Dietary Supplement Using Multi-mode Liquid Chromatography with Charged Aerosol Detection


UHPLC System for Counterion Analysis Part Number
Thermo Scientific Dionex UltiMate 3000 SD System Package including Corona Veo detector 5200.0002
Including: Solvent rack SR-3000; analytical pump LPG-3400SD; autosampler WPS-3000TSL; column compartment TCC-3000SD; UV detector VWD-3100; analytical flow cell with 11μL, 10mm, SST; Corona Veo and Chromeleon CDS software, version 7.2

 

IonCount Chromatography Starter Kit Part Number
Chromatography Starter Kit 6200.0002
Including: Acclaim Trinity P1, 3μm, 3.0 x 50mm; Acclaim Trinity P2, 3μm, 3 x 50mm; Acclaim SST Guard Holder V-2; Acclaim Trinity P1 Guard Cartridges, 3μm, 3 x 1mm; Acclaim Trinity P2 Guard Cartridges, 3μm, 3 x 1mm; Viper Capillary, ID x L 0.10 x 65mm, SST; Optima LC-MS ammonium formate; Optima LC-MS formic acid and CD with eWorkflow and Operating Instructions

 

Additional Columns  
Acclaim Trinity P1 Columns Part Number
Acclaim Trinity P1, 3μm, 3.0 x 150mm 075563
Acclaim Trinity P1, 3μm, 3.0 x 100mm 071387
Acclaim Trinity P1, 3μm, 2.1 x 150mm 075564
Acclaim Trinity P1, 3μm, 2.1 x 100mm 071389
Acclaim Trinity P1, 3μm, 2.1 x 50mm 075565
Acclaim Trinity P1, 3μm Guard column, 2.1 x 10mm 071391
Acclaim Trinity P2 Columns Part Number
Acclaim Trinity P2, 3μm, 3.0 x 100mm 085434
Acclaim Trinity P2, 3μm, 2.1 x 100mm 085431
Acclaim Trinity P2, 3μm, 2.1 x 50mm 085432
Acclaim Trinity P2, 3μm Guard column, 2.1 x 10mm 085435
 

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Dionex Corona Veo Detector: Your Hidden Peaks Revealed with Clearer Vision 

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Resources

Register now for access to the following literature:

  • Application Note: The Extraction of Banned Azo Dyes in Textiles Using HyperSep SLE
  • Application Note: The Extraction Rifabutin from Serum Using HyperSep SLE
  • Application Note: The Extraction of Oxymorphone and its Isomer from Human Plasma Using HyperSep SLE

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