Water Microbiology Testing

Microbiology products for the isolation, identification and enumeration of waterborne microorganisms to meet your testing needs.

Our range of products for water testing includes dehydrated and prepared media, membrane filters, qPCR assays for Legionella, quality control organisms, as well as the equipment you need to deliver reliable results.

  • Safe: media formulations that are compliant with ISO Standards for the testing of water
  • Conform to ISO standard: quality control testing methods for culture media are accredited in accordance with ISO 17025:2017
  • Proven and certified: all Certificates of Analysis (CoAs) for culture media confirm testing in accordance with the ISO 11133:2014 standard, including membrane filter testing
  • Support: our team of microbiology experts is at hand to help with your technical queries

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Featured products

qualyfast-range-278x140
QuantStudio5-278x140
GVPC-plate-Legionella-media-278x140

Detection of microorganisms: ISO 6222:1999

 

Water sample

Poured plate method with Yeast Extract Agar


68±4 h at 20±2 °C and 44±4 h at 36±2 °C

Count all colonies formed


Method Media Product Format Product Code
Pour plate Yeast Extract Agar Plate Count Agar for water testing (ISO) 500 g
Dehydrated culture media
CM1012B
Plate Count Agar 10 x 100 mL bottles
Prepared media
BO0055M
10 x 200 mL bottles
Prepared media
BO0055R

Detection of Escherichia coli / Coliform bacteria: ISO 9308-1:2014

 

Water sample

Membrane filtration, using filter on surface of CCA Agar

36±2 ºC 21±3 h


Count all rose colonies as coliforms

Count all dark blue to purple colonies as E.coli

Test oxidase with freshly prepared reagent

Negative: No blue colonies

Confirmed E.coli

 

> Count all Oxidase-negative bacteria as coliforms

> Count all Oxidase-positive bacteria as non-coliforms

Method Media Product Format Product code
Membrane filtration CCA Agar Thermo Scientific™ Chromogenic Coliform Agar 500 g
Dehydrated culture media
CM1205B
10 x 90 mm plates
Prepared media
PO5318A
10 x 55 mm plates
Prepared media
PO5428J
Oxidase test Tryptone Soya Agar (TSA) CASO AGEN (TSA) 500 g / 5 kg
Dehydrated culture media
CM0131B/T
10 x 90 mm plates
Prepared media
PO5012A
Oxidase Reagent Thermo Scientific™ BactiDrop™ Oxidase Reagent 50 vials
Dehydrated culture media
R21540
10 x 90 mm plates
Prepared media
PO5321A
Thermo Scientific™ Microbact™ Oxidase Strips 50 strips
Dehydrated culture media
MB0266A

Detection of Enterococci: ISO 7899-2:2000

 

Water sample

Membrane filtration, using filter on surface of Slanetz and Bartley Agar

36±2 °C 44±4 h

Count all convex red, brown or rose colonies


Put filter onto pre-warmed 44°C

Bile Aesculine Azide Agar

44±0.5°C 2 h

Enumerate all brown colonies with brown halos as intestinal Enterococci

Method Media Product Format Product code
Membrane filtration Slanetz Bartley Agar Slanetz Bartley Agar 500 g
Dehydrated culture media
CM0377B
10 x 90 mm plates
Prepared media
PO5018A

10 x 55 mm plates

Prepared media

PO5410J*

10 x 55 mm plates

Prepared media

PO5423J*
Confirmation Bile Aesculine Azide Agar

Enterococcus Selective Medium

(Bile Aesculine Azide Agar)1

10 x 90 mm plates
Prepared media
PO5062A
or Kanamycin Aesculin Azide Agar Base2 500g
Dehydrated culture media
CM0591B
10 x 90 mm plates
Prepared media
PO5059A

* Minimum order quantity required.

1. This medium is slightly different to the medium formulation according to ISO 7899-2:2000. The medium has additional sodium citrate and 20 g/l ox bile instead of 10 g/l and 0.55 g/l sodium azide instead of 0.15 g/l.

2. This medium is slightly different to the medium formulation according to ISO 7899-2:2000. It contains sodium citrate instead of ox bile and Kanamycin.


Detection of Pseudomonas aeruginosa: ISO 16266:2006

 

Water sample

Membrane filtration, using filter on surface of Pseudomonas CN Agar


36±2 °C 44±4 h*



Inspection under UV light

(360±20 nm)

Inspection under natural light

Inspection under natural light

Fluorescence colonies: presumptive Pseudomonas aeruginosa

Blue green (Pyocyanine forming) colonies: confirmed Pseudomonas aeruginosa

Red brown colonies: presumptive Pseudomonas aeruginosa

 

Subculture to Nutrient Agar

Subculture to Nutrient Agar

36±2°C 22±2 h

36±2°C 22±2 h

Add Nessler´s Reagent ammonia development (red-yellow precipitation)

Oxidase test: oxidase positive

Confirmed Pseudomonas aeruginosa

Acetamide Solution

King´s B Medium

 

36±2°C 22±2 h

36±2°C up to 5 days

Add Nessler´s Reagent ammonia development (red-yellow precipitation)

Fluorescence under UV light

Confirmed Pseudomonas aeruginosa

* Minimum order quantity required.

Method Media Product Format Product code
Membrane filtration Pseudomonas Selective Agar / CN Agar Pseudomonas Centrimide Selective Agar 10 x 90 mm plates
Prepared media
PO5076A
10 x 55 mm plates
Prepared media
PO5413J*
Pseudomonas Agar Base 500 g
Dehydrated culture media
CM0559B
10 tubes
Dehydrated culture media
SR0102E
Confirmation
Fluorescence King´s B Media      
Hydrolysis of acetamide to ammonia Acetamide Solution Nessler Reagent      
Oxidase Test Nutrient Agar Nutrient Agar 500 g
Dehydrated culture media
CM0003B
Oxidase Reagent BactiDrop Oxidase Reagent 50 vials
Dehydrated culture media
R21540
Microbact Oxidase Strips 50 strips
Dehydrated culture media
MB0266A

* Minimum order quantity required.


Detection of Clostridium perfringens (including spores): ISO 14189:2013

 

Membrane filtration of 100 mL onto TSC-Agar* (if necessary, dilute sample)

44±1°C, anaerobic 21±3 h

Enumerate the presumptive colonies which show black or grey to yellow brown staining within 30 minutes of opening

Count all black colonies as Clostridium perfringens


1-10 black or grey to yellow-brownish colonies

>10 black or grey to yellow-brownish colonies

Subculture all presumptive colonies onto blood agar plates 

Subculture 10 different presumptive colonies onto blood agar plates

36±2°C, anaerobic 21±3 h

36±2°C, anaerobic 21±3 h

Put colonies that grew under anaerobic conditions onto filter paper and add 2-3 drops of phosphateacid. All colonies that turn purple within 3-4 minutes are confirmed positive.


*ATTENTION: Alternatively, a thin layer (approximately 5 mL to 10 mL) of molten TSC Agar (TV5204G TSC Agar Base), as an overlay on the filter can be used. Allow to solidify before anaerobic incubation. This method may enhance the blackening of the colonies. Medium without cycloserine; e.g. TV5204G TSC Agar Base, 20 mL.

Melt TSC Agar in a water bath and put melted agar onto filter on the plate:

 

Melt agar in a water bath at 95°C for approx. 10-15 minutes

Cool medium to 45°C

Shake tube carefully and pour a thin layer of molten agar

Method Media Product Format Product code
Membrane filtration TSC Agar TSC Selective Agar 10 x 90 mm plates
Prepared media
PO5315A
TSC Agar Base + 500 g
Dehydrated culture media
CM0587B
50 tubes
Prepared media
TV5204G
TSC Selective Supplement 10 tubes
Dehydrated culture media
SR0088E
10 x 100 mL bottles
Prepared media
BO0634M
10 tubes
Prepared media
SR0088E
Anaerobic atmosphere   Thermo Scientific™ AnaroGen™ System 10 sachets
AN0025A
Thermo Scientific™ AnaroJar™ 2,5 L Anaerobic System 1 unit
AG0025A
Thermo Scientific™ AnaeroBox™ Rectangular Anaerobic System 1 unit
AB0025A

Detection of Legionella: ISO 11731:2017

Sample preparation:

Heat treated: Put sample into water bath for 30+/-2 minutes at 50 °C

Acid treated: 1 part sample to 9 parts of acid buffer. Mix well and leave it for 5+/- 0.5 minutes.

Acid treated filter: Put 30 mL of acid solution onto the membrane filter. Leave it for 5 +/- 0.5 minutes and remove the solution by filtration. Wash with 20 mL of either sterile Aqua dest., Pages Saline, Ringer Solution or PBS.

Samples with high concentration of Legionella species and low concentration of interfering organisms2

Samples with low concentration of Legionella species and low concentration of interfering organisms2

0.1-0.5 mL of the sample onto BCYE 

0.1-0.5 of the sample onto BCYE with AB

Membrane filtration of 100 mL sample1.

Place one untreated membrane filter onto BCYE Agar; Place membrane filter with acid solution on one or more selective media: BCYE Agar with AB or GVPC Agar or MWY Agar

0.1-0.5 mL of the sample; each untreated, heat treated and acid treated from the membrane filtration with washing procedure3 onto BCYE Agar and on one or more selective media: BCYE Agar with AB or GVPC Agar or MWY Agar

36 ± 2 °C, humid atmosphere, 7-10 days

Subculture at least 3 typical Legionella colonies from each plate and inoculate on BCYE Agar without Cystein and BCYE Agar

36 ± 2 °C, humid atmosphere, 2-5 days

Colonies that grow on BCYE but not on BCYE without cystein are considered to be Legionella3

 
  1. 10 – 1000 mL sample volume according to ISO 11731-2.
  2. The choice of the method for the enumeration of Legionella species depends on the origin/characteristics of the sample and the reason for sampling or investigation. A decision matrix can be found in the ISO 11731:2017, Annex J.
  3. Further information, please see ISO 11731:2017.
Method Media Product Format Product code
Sample preparation Acid Buffer Legionella Acid Buffer Solution 6 x 1 L bottles
Prepared media
GFB01
Direct and membrane filtration Legionella GVPC Agar Legionella GVPC Selective Agar 10 x 90 mm plates
Prepared media
PO5074A
10 x 90 mm plates
Prepared media
PO0245A
Legionella CYE Agar-Base + 500 g
Dehydrated culture media
CM1203B
Legionella BCYE Supplement + 10 tubes (500 mL)
Dehydrated culture media
SR0251C
Legionella GVPC Selective Supplement 10 tubes (500 mL)
Dehydrated culture media
SR0252E
Legionella GVPC Agar with antibiotics Legionella BCYE Agar with antibiotics 10 x 90 mm plates
Prepared media
PO5325A
Legionella BCYE Agar Legionella BCYE Agar 10 x 90 mm plates
Prepared media
PO5072A
Legionella CYE Agar Base + 500 g
Dehydrated culture media
CM1203B
Legionella BCYE Supplement 10 tubes (500 mL)
Dehydrated culture media
SR0251C
Legionella MWY Agar Legionella MWY Agar 10 x 90 mm plates
Prepared media
PO5071A
Legionella CYE Agar Base + 500 g
Dehydrated culture media
CM1203B
Legionella BCYE Supplement + 10 tubes (500 mL)
Dehydrated culture media
SR0251C
Legionella MWY Selective Supplement 10 tubes (500 mL)
Dehydrated culture media
SR0255B
Subculture Legionella BCYE Agar without Cysteine Legionella BCYE Agar without Cysteine 10 x 90 mm plates
Prepared media
PO5028A
10 x 90 mm plates
Prepared media
PO0255A*
Legionella CYE Agar Base + 500 g
Dehydrated culture media
CM1203B
Legionella BCYE without Cysteine Supplement 10 tubes (100 mL)
Dehydrated culture media
SR0253A
Legionella BCYE Agar Legionella BCYE Agar 10 x 90 mm plates
Prepared media
PO5072A
Legionella CYE Agar Base + 500 g
Dehydrated culture media
CM1203B
Legionella BCYE Supplement 10 tubes (500 mL)
Dehydrated culture media
SR0251C

* Minimum order quantity required.


Detection of Legionella: qualyfast® qPCR

Detection of Legionella qualyfast qPCR workflow
Product Format Order code
qualyfast Reagents and PCR Kits
qualyfast Free DNA Inactivator 200 reactions D401009
qualyfast DNA Extraction Kit I clear water 24 reactions D101001
48 reactions D201001
96 reactions D301001
qualyfast DNA Extraction Kit II dirty water 48 reactions D211001
96 reactions D311001
qualyfast Legionella PCR Assay 24 reactions
(3x 8-tube strips)
A101013
48 reactions
(6x 8-tube strips)
A201013
96 reactions
(12x 8-tube strips)
A301013
qualyfast Legionella Quantification Standards 8 tests
(4 standards each in an 8-tube strip)
A501013
QuantStudio 5 Food Safety PCR System
Applied Biosystems™ QuantStudio 5 Food Safety Real-Time PCR System, 0.1 mL Block, Laptop System A36328
Applied Biosystems QuantStudio 5 Food Safety Real-Time PCR System, 0.1 mL Block, Desktop System A36320
Service & Support Packages
Applied Biosystems Assurance Service Plan
additional 1 year warranty cover with instrument loan
1 year cover SCQS5FAST

Filter Funnels and Membranes

Thermo Scientific™ Nalgene™ Membrane Filters are cellulose nitrate membranes that are certified for microbiological QC testing and analysis of water.

Product Size of Quantity Catalog Number
Nalgene Disposable Analytical Funnels equipped with membrane filter
Sterile Analytical Filter Units, 100 mL, 47 mm, 0.45 μm, white/black 50 pieces NG145-0045
Sterile Analytical Filter Units, 250 mL, 47 mm, 0.45 μm, white/black 50 pieces NG145-2045
Sterile Analytical Filter Units, 100 mL, 47 mm, 0.45 μm, grey/black 50 pieces NG147-0045
Sterile Test Filter Funnel, 250 mL, 0.45 uL 50 pieces NG147-2045
Membrane filter
Membrane Filter for water testing, sterile, CN, 47 mm, 0,45 μm, white/black 100 pieces NG0205-4045
Membrane Filter for water testing, sterile, CN, 47 mm, 0,45 μm, grey/black 100 pieces NG0210-6045
Accessories
Vacuum Manifold 1 piece NG0345-0001
Filter Stopper, non-sterile, no. 8 rubber 3 pieces NG0396-0080
Filter Forceps, bent tip 1 piece NG0399-0001
Filter Forceps, straight tip 1 piece NG0399-0002
Filter Funnel Adapter, non-sterile 25 pieces NG0397-0010
Vacuum Gasket, non-sterile thermoplastic elastomer 6 pieces NG0395-0708
Nalgene Reusable Analytical Filters and accessories
Filter Funnels with Clamp, 250 mL 1 piece NG0315-0047
Filter Holders with Receiver, 500 mL, 500 mL 4 pieces NG0300-4000
Filter Holders with Receiver, 250 mL, 250 mL 4 pieces NG0300-4050
Filter Holders with Receiver, 500 mL, 1000 mL 4 pieces NG0300-4100
Filter Holders with Funnel, 250 mL 1 piece NG0310-4000
Filter Holders with Funnel, 500 mL 1 piece NG0310-4050
Reusable Bottle Top Filters, 250 mL 1 piece NG0320-2545
Reusable Bottle Top Filters, 500 mL 1 piece NG0320-5033
Reusable Bottle Top Filters, 500 mL 1 piece NG0320-5045

Quality Control organisms: ISO 11133:2014

Product Sample numbers Catalog Number
Escherichia coli QC Organisms for testing of CCA Agar
Escherichia coli ATCC® 8739™* WDCM 00012 R4607085
Escherichia coli ATCC® 25922™* WDCM 00013 R4607050
Enterobacter aerogenes ATCC® 13048™* WDCM 00175 R4607080
Pseudomonas aeruginosa ATCC® 27853™* WDCM 00025 R4607060
Pseudomonas aeruginosa ATCC® 9027™* WDCM 00026 R4605210
Enterococcus faecalis ATCC® 19433™* WDCM 00009 R4601990
Enterococcus faecalis ATCC® 29212™* WDCM 00087 R4607030
Enterococci QC Organisms for testing of Slanetz and Bartley Agar
Escherichia coli ATCC® 8739™* WDCM 00012 R4607085
Escherichia coli ATCC® 25922™* WDCM 00013 R4607050
Staphylococcus aureus subsp. aureus ATCC® 6538™* WDCM 00032 R4607016
Staphylococcus aureus subsp. aureus ATCC® 25923™* WDCM 00034 R4607010
Enterococcus faecalis ATCC® 29212™* WDCM 00087 R4607030
Enterococcus faecalis ATCC® 19433™* WDCM 00009 R4601990
Pseudomonas aeruginosa QC Organisms for testing of Pseudomonas CN Agar
Pseudomonas aeruginosa ATCC® 27853™* WDCM 00025 R4607060
Pseudomonas aeruginosa ATCC® 9027™* WDCM 00026 R4607060
Pseudomonas aeruginosa ATCC® 10145™* WDCM 00024 R4607065
Escherichia coli ATCC® 8739™* WDCM 00012 R4607085
Escherichia coli ATCC® 25922™* WDCM 00013 R4607050
Enterococcus faecalis ATCC® 19433™* WDCM 00009 R4601990
Enterococcus faecalis ATCC® 29212™* WDCM 00087 R4601990
Clostridium perfringens QC Organisms for testing of TSC Agar
Bacillus subtilis ATCC 6633 WDCM 00003 R4601221
QC organisms for testing GCVP Agar (Legionella method)
Legionella pneumophila ATCC® 33152™* WDCM 00107 R4603950
Legionella anisa NCTC 11974™ WDCM 00106 R4601315
Enterococcus faecalis ATCC® 19433™* WDCM 00009 R4601990
Enterococcus faecalis ATCC® 29212™* WDCM 00087 R4607030
Pseudomonas aeruginosa ATCC® 27853™* WDCM 00025 R4607060
Pseudomonas aeruginosa ATCC® 9027™* WDCM 00026 R4605210
Escherichia coli ATCC® 8739™* WDCM 00012 R4607085
Escherichia coli ATCC® 25922™* WDCM 00013 R4607050
Prepared Media for the Quality Control testing according to ISO 11133:2014
CASO Agar (ISO 11133)   PO5321A

 

* The ATCC Licensed Derivative Emblem, the ATCC Licensed Derivative word mark, and the ATCC catalog marks are trademarks of ATCC. Remel Inc. is licensed to use these trademarks and sell products derived from ATCC® cultures. The identity, purity, and authenticity of the Licensed Products are exclusively the responsibility of Remel Inc., and not ATCC.


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