Combat food fraud with a complete next generation sequencing workflow for in-house multi-species ID

Combat food fraud with a complete next generation sequencing workflow for in-house multi-species ID

Having confidence in the authenticity of your food ingredients is essential to protecting your brand against food fraud: a topic of increasing concern in the food industry and an area highly regulated by authorities through food labelling regulations. Food fraud costs the food industry around $15 billion globally1, 2 however the complexity of the food supply chain is challenging the abilities of analytical tools used for traceability of ingredients. The Thermo Scientific™ Next Generation Sequencing (NGS) Food Authenticity Workflow brings the power of NGS technology to your lab making it possible to identify the plant, fish and meat species contained in the most complex food samples. Let us help you to protect your customers and your reputation against intentional, or accidental, adulteration.

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Popular NGS Food Species Screening Products

SGS™ All Species ID Food DNA Analyzer Kits
Ion Chef™ Food Protection Instrument
Ion GeneStudio™ S5 Food Protection System

Initiate in-house species testing for rapid answers with a complete, simple NGS workflow

Identification of the species present in food and feed samples is a critical step for proof of authenticity, verification of origin, traceability of raw materials, and quality control of handling and cleaning processes in food production lines.

The Thermo Scientific NGS Food Authenticity Workflow is a new solution for verifying and authenticating products for improved traceability and food safety. It brings together SGS™ All Species ID Food DNA Analyser Kits for meat, fish and plant species with the Thermo Fisher Scientific™ Ion Torrent™ Next Generation Sequencing platform and the SGS™ All Species ID Software. Barcoding of samples with exclusive barcoded DNA sequences enables the analysis of multiple food and feed samples in a single run.

Simple: streamlined NGS workflow with automated DNA extraction & amplification, library construction, sequencing and data analysis

Comprehensive: screening of food samples against an extensive database of meat, fish and plant species, including exotic variants, gives accurate detection and differentiation of expected and unexpected species

Rapid: sample to result in as little as one day

NGS Food Workflow

The most common molecular method to verify species substitution and species identification is real-time PCR. However, PCR testing is limited by the number of targets that can be simultaneously identified and differentiated and requires knowledge about which species to search for. This can be critical, especially when testing highly processed and complex foods that often contain multiple different species.

The introduction of Next Generation Sequencing (NGS) into the food sector revolutionizes food authenticity testing. An NGS untargeted approach enables accurate detection and differentiation of thousands of different species in each sample using DNA sequencing which is recognized as the most reliable method for species identification.

At the end of an NGS analysis, millions of individual sequences are obtained making it possible to identify species in complex foods containing multiple ingredients. This is because each DNA-containing ingredient will produce a single and unique DNA sequence. The sequences obtained can be compared against a database resulting in a complete list of all the species present in the sample.

There is no requirement for previous knowledge of the supply chain, or which targets to search for. This means that now the question can be simply; “which meat species are in my sausage meat?”, or “are there any other plants in my dried oregano”, and “can I defend my label claims?”

NGS Food Authenticity Workflow Products

Catalog # Name
Ion Torrent Food Protection Instruments

A39513

Ion GeneStudio™ S5 Food Protection System

A39514

Ion Chef™ Food Protection Instrument

Low Throughput Gel Electrophoresis 

G8342ST

E-Gel™ Power Snap Electrophoresis System Starter Kit, EX 2%

High Throughput Gel Electrophoresis

4466611

E-Gel™ Imager System with UV Light Base

EBM03

Mother E-Base™ Device

EBM03EU

Mother E-Base™ Device (European version)

EBM03UK

Mother E-Base™ Device (UK Version)

EBD03

Daughter E-Base™ device

Quantification

A39533

Qubit™ Food Protection Starter Pack

Amplification

A24811

SimpliAmp™ Thermal Cycler

Purification

12321D

DynaMag™-2 Magnet

   
DNA Extraction

4466336

GMO Extraction Kit

   
Library Preparation

A38452

SGS™ All Species Meat DNA Analyser Kit I

A38453

SGS™ All Species Meat DNA Analyser Kit II

A38454

SGS™ All Species Fish DNA Analyser Kit I

A38455

SGS™ All Species Fish DNA Analyser Kit II

A38456

SGS™ All Species Plant DNA Analyser Kit I

A38457

SGS™ All Species Plant DNA Analyser Kit II

A38458

SGS™ All Species ID Software

   
Qubit Consumables  

Q32856

Qubit Assay Tubes

Q32853

Qubit dsDNA BR Assay Kit

   
Low Throughput Gel Electrophoresis   

G401002

E-Gel™ EX Agarose Gels, 2%

10482055

E-Gel™ Sample Loading Buffer, 1X

10488099

E-Gel™ 50 bp DNA Ladder

   
High Throughput Gel Electrophoresis  

G800802

E-Gel™ 48 Agarose Gels, 2%

G700802

E-Gel™ 96 Agarose Gels, 2%

12373031

E-Gel™ Low Range Quantitative DNA Ladder

10482055

E-Gel™ Sample Loading Buffer, 1X

10488099

E-Gel™ 50 bp DNA Ladder

   
Sequencing Consumables  

A39476

Ion 510™ & Ion 520™ & Ion 530™ Food Protection Kit – Chef (1 sequencing run per initialization)

A39477

Ion 510™ & Ion 520™ & Ion 530™ Food Protection Kit – Chef (2 sequencing runs per initialization)

A27769

Ion 510™ Food Protection Chip Kit

A39473

Ion 520™ Food Protection Chip Kit

A39478

Ion S5™ Food Controls Kit Plus

   

1 GMA (2010) “Consumer product fraud: Deterrence and detection.”   2 Kircher, A. (2012) “Tools for protecting the nation’s food supply,” National Center for Food Protection and Defense.

 

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