Carbohydrate Testing Information

Affecting taste and nutrition

The determination of the types and concentrations of carbohydrates in foods is integral for energy evaluation, nutritional labeling, quality control, and for identifying possible adulteration. We've consolidated the application notes you need on a range of topics, including corn syrup, dietary fiber, lactose and lactulose, maltodextrins, sialic acids and simple carbohydrates.


HPAE-PAD provides sensitive, direct determination without sample derivatization for carbohydrate analysis. 


The Dionex ICS-5000+ performs a simple oxidation of a small amount of the carbohydrate so that it can be separated as anions.

Application note

Rapid analysis of natural sweeteners found in food and beverages using an advanced UHPLC system

A method to demonstrate the full resolution of six steviol glycosides using an alternative HILIC-based method.


The Ideal Solution for Simple Sugar Analysis

Why it’s important to test carbohydrate levels in foods and beverages and how to do it accurately

Customer application note

Sugars in Honey Using HPAE-PAD: What is the Best Column?

A method to separate and determine fructose, glucose, sucrose, turanose, maltose, trehalose, isomaltose, erlose, raffinose, and melezitose in different honey samples using HPAE-PAD


Sugar Analysis

Fully integrated sugar analyzer for food and beverage samples.

Featured webinars

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Resource Number
Application Note Determination of Carbohydrates in Fruit Juice Using Capillary HPAE-PAD Ion Chromatography AB127
Application Note Profiling Galactosyloligosaccharide Containing Samples by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD) Ion Chromatography AN1151
Application Note Determination of Plant-Derived Natural Oligo- and Polysaccharides Ion Chromatography AN67
Application Note Analysis of Fruit Juice Adulterated with Medium Invert Sugar Ion Chromatography AN82
Application Note Determination of Trans-Galactooligosaccharides in Foods by AOAC Method 2001.02 Ion Chromatography AN155
Application Note Determination of Lactose in Lactose-Free Milk Products by HPAE-PAD Ion Chromatography AN248
Application Note HPAE-PAD Determination of Infant Formula Sialic Acids Ion Chromatography AN253
Application Note Carbohydrate in Coffee: AOAC Method 995.12 vs a New Fast Ion Chromatography Method Ion Chromatography AN280
Application Note Determination of Sialic Acids Using UHPLC with Fluorescence Detection Liquid Chromatography AN266
Application Notebook Carbohydrate Analysis with High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection Ion Chromatography AI71132
Application Notebook Carbohydrates Analysis -Novel, Selective, Sensitive Analytical Methods Ion Chromatography AI71469
Application Update Measuring Lactose in Milk: A Validated Method Ion Chromatography AU182
Brochure Complete Solution for Testing Sugars in Wine and Juice with Automated Discrete Analyzers, System Reagent Kits and Standard Solutions Discrete Analysis BR71488
Brochure Carbohydrate Analysis with HPAE-PAD Ion Chromatography BR52119
Case Study Quick and Easy Testing for a Finnish Confectionary Discrete Analysis CS70816
Technical Note Determinations of Monosaccharides and Disaccharides in Beverages by Capillary HPAE-PAD Ion Chromatography TN135
Webinar Biochemistry of Carbohydrates (Education Webinar) Additional Techniques  
Application Update Determination of Plant-Derived Neutral Oligo- and Polysaccharides Ion Chromatography AU150
Application Note Profiling Fructooligosaccharide containing Samples by HPAE-PAD Ion Chromatography AN1149
White Paper Simplifying Carbohydrate Testing to Meet Food Quality and Labeling Requirements Using Ion Chromatography and Pulsed Amperometric Detection Ion Chromatography WB71806
Article Sugar Analysis Document: Thermo Fisher Scientific on challenges in sugar analyses Ion Chromatography  
White Paper Innovations in Carbohydrate Analysis Additional Techniques WP72985
White Paper Carbohydrate Analysis of Agave Syrup using HPAE-PAD Additional Techniques AN73132
Application Note Profiling Galactosyloligosaccharide-Containing Samples by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD) Ion Chromatography AN71993