Listeria species and Listeria monocytogenes testing solutions

The detection of Listeria in foods before they are consumed is vital for safeguarding public health and essential for preserving the financial health and reputation of food businesses. When performing testing, the presence of Listeria species is often considered indicative that pathogenic Listeria monocytogenes may also be present. Therefore, when ensuring that Listeria monocytogenes is absent or present below a certain level in tested samples, the strategy often involves the detection of Listeria  species in general. We offer a wide range of products and solutions for the detection, enumeration, and identification of Listeria in foods and associated sample types.


Reference methods

ISO 11290:2017 standard method

A horizontal method for the detection (Part 1) and enumeration (Part 2) of Listeria monocytogenes and Listeria spp. cited in Commission Regulation (EC) No 2073/2005 and other national regulations and guidelines.

FDA BAM Chapter 10: Method for detection and enumeration of Listeria monocytogenes

A culture media-based standard reference method cited in the U.S Food & Drug Administration Bacteriological Analytical Manual.

USDA FSIS MLG 8.14: Isolation and identification of Listeria monocytogenes

A culture-media based standard reference method cited in the United States Department of Agriculture (USDA) Food Safety and Inspection Service (FSIS) Microbiology Laboratory Guidebook (MLG) Method Number 8.14.


PCR-based rapid methods

Thermo Scientific SureTect Listeria monocytogenes PCR Assay

A rapid real-time PCR method for the detection of Listeria monocytogenes in a broad range of foods and environmental samples. The assay's workflow holds AOAC Performance Tested Methods(SM) approval granted by the AOAC Research Institute and is an AOAC Official Method(SM) with Final Action status granted by AOAC International. The assay is also validated according to ISO 16140-2:2016 and certified by NF Validation™ by AFNOR Certification.

Thermo Scientific SureTect Listeria species PCR Assay

A rapid real-time PCR method for the detection of Listeria species in a broad range of foods and environmental samples. The assay's workflow holds AOAC  Performance Tested Methods(SM) approval granted by the AOAC Research Institute and is an AOAC Official Method(SM) with Final Action status granted by AOAC International. The assay is also validated according to ISO 16140-2:2016 and certified by NF Validation™ by AFNOR Certification.


Chromogenic culture media-based methods

Thermo Scientific Listeria Precis Detection Method

A streamlined, rapid, culture based method to detect Listeria species and Listeria monocytogenes in foods and environmental samples. The workflow holds AOAC Performance Tested Methods(SM) approval granted by the AOAC Research Institute and is an AOAC Official Method(SM) with Final Action status granted by AOAC International. The method is also validated according to ISO 16140-2:2016 and certified by NF Validation™ by AFNOR Certification.

Thermo Scientific Listeria Precis Enumeration Method

A streamlined, rapid, culture based method to enumerate Listeria species and Listeria monocytogenes in foods and environmental samples. The workflow holds AOAC Performance Tested Methods(SM) approval granted by the AOAC Research Institute and is an AOAC Official Method(SM) with Final Action status granted by AOAC International. The method is also validated according to ISO 16140-2:2016 and certified by NF Validation™ by AFNOR Certification.


Reduce the risk of human error in your PCR-based Listeria testing

Mistakes in your PCR workflow can lead to false-positive or false-negative results, compromising the accuracy and reliability of the test. With the Thermo Scientific SureTect Automation Workflow you can take your PCR-based Listeria testing to the next level with automated lysis and PCR setup. Utilizing the CyBio FeliX Instrument, the automated workflow allows you to reduce hands-on time while increasing the accuracy and reproducibility of results.

Streamlined Listeria enumeration                                 

A recent addition to the Listeria Precis Enumeration Workflow, our bottle format is designed specifically for pour plating, exhibiting equivalence to the ISO 11290-2:2017 reference method and providing up to 10x increased sensitivity, allowing you to accurately detect even the lowest levels of contamination. Unlike surface-plating methods that need three plates to achieve necessary sensitivity, pour plating uses a single plate per sample, saving on costs.