Salmonella Food Testing Resources

Learn more about Salmonella food testing with the latest information and resources

Posters and application notes

Poster: Thermo Scientific SureTect Salmonella species PCR Assay Method ISO 16140-2:2016 Matrix Extensions

Learn how the Thermo Scientific SureTect Salmonella Species PCR Assay underwent a successful extension to increase the scope of its matrices in line with ISO 16140-2:2016, adding pet food and animal feed matrices and enhancing protocols for meat, vegetable and powdered infant formula (PIF) categories.

Poster: Evaluation of a PCR workflow for the detection of Salmonella from pooled chocolate ingredients

The purpose of the study was to evaluate the Thermo Scientific SureTect Salmonella species PCR workflow for the detection of Salmonella from chocolate ingredients after a post enrichment pooling step.

Poster: Detection, Quantitation, and Serotyping of Salmonella from Poultry Samples with Harmonized Protocols

This poster demonstrates the harmonization of test portion dilution and enrichment for raw poultry products and poultry rinses using the Thermo Scientific SureCount Salmonella Species, Typhimurium and Enteritidis Multiplex PCR Kit, Thermo Scientific SureTect Salmonella species PCR Assay, Thermo Scientific SureTect Salmonella species, Typhimurium and Enteritidis Multiplex PCR Assay, and Thermo Scientific SureTect Salmonella Infantis PCR Assay.

Poster: Salmonella species Detection in Pet Food Using a Well-Established Real-Time PCR Method

This study demonstrates the accuracy and reliability of the SureTect Salmonella PCR method for the detection of Salmonella in large sample sizes of pet food.

Poster: Harmonized AOAC PTM PCR Detection Workflows for Salmonella and STEC from 375 g Raw Beef and Leafy Produce Samples

This study involved the development and analysis of a harmonized protocol for 375 g raw beef trim and leafy produce samples. 

Poster: Harmonized large sample size (375 g) testing for detection of Salmonella and Cronobacter spp. in Powdered Infant Formula Using Thermo Scientific SureTect Salmonella PCR Assay and Thermo Scientific SureTect Cronobacter PCR Assay certified by AOAC PTM

This study evaluated the Thermo Scientific SureTect Salmonella species PCR Assay and Thermo Scientific SureTect Cronobacter species PCR Assay harmonized enrichment for large PIF sample sizes and environmental samples.

Application note: Quantitation, detection, and differentiation of Salmonella from poultry

Application note: Evaluating the performance of the SureTect Salmonella species PCR assay to detect Salmonella spp. from PPS samples when Tetrathionate (TT) Broth and a regrowth step is used as the enrichment procedure

Application note: Harmonized Salmonella and Cronobacter testing in powdered infant formula and related matrices

Application note: Testing beef trim sampling cloths for Escherichia coli O157:H7, STEC, and Salmonella species with harmonized enrichments

Our comprehensive suite of solutions offer a robust and efficient workflow for detecting E. coli O157:H7, non-O157 STEC serotypes, and Salmonella species from beef trim sampling cloths. A single sample enrichment for all four PCR assays streamlines the testing process, enhancing efficiency. Additionally, the coinoculation of sampling cloths with both Salmonella and STEC simulates the worst-case real-world scenario, proving method performance and ensuring reliable detection. 

Thermo Scientific SureTect Salmonella Infantis Assay method: Verification study report

These studies evaluated the performance of the SureTect Salmonella Infantis PCR Assay workflow for the detection of Salmonella Infantis from food samples.

Webinars

A serotype-specific PCR method for detecting Salmonella spp. in poultry

Speaker: Dr. Frieda Jørgensen, Senior Scientist, Health Protection England

Key learning points:

  • The limitations of conventional culture methods for samples co-contaminated with several serovars
  • The assay’s ease of laboratory uptake, robust calling of results, and reliable performance, regardless of whether applied to primary or secondary enrichments, for the confirmation of isolates, or use on challenging matrices
  • Straightforward serotype-specific PCR in samples that would otherwise have evaded detection
  • Rapid and more comprehensive identification of affected products for more effective intervention

Impact of Salmonella on the global meat and poultry supply chain

Speakers: Erin Crowley, Chief Scientific Officer at Q Laboratories, Inc., and Martin Wiedmann, Gellert Family Professor in Food Safety at Cornell College of Agriculture and Life Sciences

Key learning points:

  • Salmonella pathogenicity and transmission from animals to humans
  • Method performances assessment according to AOAC Guidelines and ISO 16140-2:2016 standard
  • An overview of the U.S. National Poultry Improvement Plan (NPIP)

The importance of Salmonella as a zoonotic agent in poultry and pork production, and the current European Regulation to fight against specific Salmonella serotypes.

Managing microbiological safety and quality of low moisture foods and ingredients

Speaker: François Bourdichon, Principal consultant for Food Safety, Microbiology and Hygiene (France)

Key learning points:

  • An overview of regional and national regulations
  • The benefits of having a harmonized workflow across multiple pathogens on a single platform

Coloring the path to food safety: Streamlined chromogenic media workflows for high throughput labs

Speakers:  

  • David Crabtree, R&D Manager, Culture Media and Food Safety Product Development, Thermo Fisher Scientific
  • Jaakko McVey, Senior Product Manager, Microbiology, Thermo Fisher Scientific
  • Sandra Freville, Regional Tactical Marketing Manager, Thermo Fisher Scientific

Key learning points:

  • Understanding chromogenic media and how it works 
  • Key benefits and selection for high-throughput labs 
  • How to evaluate for implementation in-house 
  • Case study: successful implementation of chromogenic workflows in a high-throughput food testing lab

Brochures

Visibly simple food pathogen testing

Thermo Scientific SureTect PCR System – simple, rapid workflows designed to get results.

SureTect Automation Platform overview

Enhance your pathogen detection using the SureTect Automation Workflow for the ultimate in lab efficiency.

Precis Methods overview

More precise foodborne pathogen detection and enumeration with next generation culture media.

Salmonella Precis method

Validated detection and identification of Salmonella species in 2 days.

Poultry testing solutions overview

Pathogen risk and quality indicator management in the poultry sector.

Rapid Salmonella detection and serotyping in poultry

A simple solution for improved risk assessment and management of Salmonella in poultry production.

Articles and more

Food for thought: Advancing food safety with PCR-based Salmonella screening

Ensuring food safety and quality is pivotal in the food industry. Salmonella, a persistent foodborne pathogen, poses a continuous threat to public health and business reputation. Recent advances in Polymerase Chain Reaction (PCR) technology are revolutionizing detection and management of this hazard. In this entry of Food for Thought, we discuss why PCR-based Salmonella screening makes economic sense, especially in light of the latest USDA regulation updates.

Food for thought: Why PCR-based Salmonella screening makes the best economic sense

Thanks to its speed and accuracy, polymerase chain reaction (PCR) testing is changing the face of Salmonella screening. Yet despite its well-documented benefits, many laboratories have been put off by the perception that PCR is expensive to adopt and complex to conduct.

Food for thought: Taking Salmonella prevention back to basics with five lessons from recent outbreaks

We know that food testing laboratories take their responsibility to screen their products for potentially dangerous pathogens seriously. But with so much on the line, from public health to brand reputation, we can never be too careful. That’s why we’re taking you back to basics with our five top tips for Salmonella prevention, based on what we’ve learnt from recent outbreaks.

Food for thought: Keeping Salmonella out of the food chain

Despite our understanding of how Salmonella proliferate in our food manufacturing processes, the bacteria still cause millions of illnesses and hundreds of deaths every year. In this special Spotlight, we take a deep dive into Salmonella, its serotypes and prevalence, and how to keep it out of your facilities, your products, and your customers.

Faster path to actionable answers for poultry producers

Thermo Fisher Scientific’s test is the first validated multiplex PCR workflow for the most common – and dangerous – Salmonella serotypes and offers a multi-target approach that can cut costs, reduce delays, and increase confidence for poultry and pork producers.

Latest validation offers food laboratories additional layer of confidence when testing for Salmonella

The SureTect Salmonella species PCR Assay has been granted AOAC Official Method Analysis (OMA) status.

Streamlined Salmonella testing drives efficiencies, enables flexibility, and solves problems

Extended validation of the Thermo Scientific Salmonella Precis Method simplifies workflows and encompasses challenging food matrices. A quick and easy culture-based method for detection of Salmonella species has been validated for the broadest range of food, animal feed, and environmental samples, and is ready to slot into standard laboratory workflows.

For Research Use Only. Not for use in diagnostic procedures.