Maximize results with the best tool for the job
Whether you're processing routine samples, highly degraded samples, or urgent request cases, Thermo Fisher Scientific provides best-in-class DNA analysis solutions to the forensic community. Choose from the proven performance of our family of genetic analyzers, the power of NGS, or the simplicity of rapid DNA to get more answers from your samples as efficiently as possible.
Count on our 3,700+ sales, service, and technical support specialists to provide answers that add simplicity, scalability, and speed to your workflow—enabling you to analyze your most difficult cases with complete confidence.
Building a forensic DNA analysis lab
Liz Peters from Fiji Police Force discusses how bringing new forensic DNA technology into her lab has improved sample processing to help solve crime faster.
Identification of human remains from rebel’s graves: CE and NGS analyses
Forensic scientists at the State Forensic Medicine Service are working to solve one of Lithuania’s greatest historical mysteries—identifying the remains from an 1863 uprising. Marija Caplinskiene, MD, PhD, discusses how her team is using both capillary electrophoresis and next-generation sequencing to analyze DNA from recovered bones found in recently revealed graves.
Forensic DNA analysis of highly degraded samples
From understanding how body decomposition affects forensic DNA analysis to simulating IED explosions with the goal of finding touch amounts of DNA, Sheree Hughes-Stamm, PhD from Sam Houston State University describes her work on optimizing DNA extraction and STR analysis methods, and when to apply next-generation sequencing to her studies.
Which capillary electrophoresis system is right for your STR analysis?
Easy-to-use, sample-to-answer system
Easy-to-use, flexible system
Meets the needs of validated and process controlled environments
|Number of capillaries/cartridges||1||4||8 (3500), 24 (3500xL)|
|Ease of use||Simple—no pipeting required||Easy, with one click universal cartridge||Moderate- some maintenance required|
|Number of dyes||6-dye enabled||8-dye enabled||8-dye enabled|
|Polymer type||Proprietary polymer||POP-1 integrated into universal cartridge||POP-6, POP-7, POP-4|
|Sample capacity||1||1 standard 96-well plate or 12 standard 8-strip tubes||2 sample plates (96- or 384-well)|
|Applications||HID applications||HID applications, fragment analysis, and sequencing||HID applications, fragment analysis, and sequencing|
|HID applications run time||90 minutes sample to answer||~39 minute CE run||30 min CE run|
|Maximum HID applications throughput (samples/day)**||16||147||384 (3500), 1,152 (3500xL)|
|HID kits accepted||Sample cartridge includes STR master mix for GlobalFiler Express and NGM Select kits||All STR kits from all vendors||All STR kits from all vendors|
|Sample type||Casework: RapidINTEL cartridge||Casework: purified DNA||Casework: purified DNA|
|Database: Rapid ACE cartridge||Database: swab, treated or untreated paper||Database: swab, treated or untreated paper|
|User level of experience||Technician: forensic lab experience not required||Trained forensic scientist||Trained forensic scientist|
Featured forensic DNA analysis products
The Applied Biosystems RapidHIT ID System generates lab-quality forensic DNA profiles in only 90 minutes with one minute of hands-on time from single-source samples. Producing STR profiles has never been this fast and easy. The compact, easy-to-use RapidHIT ID System can be set up in a day in virtually any setting, and accompanying Applied Biosystems RapidLINK Software enables real-time access, review and control.
The Applied Biosystems 3500 Series genetic analyzers now offer new features for improved data interpretation and user experience. The complete system combines the instrument with Applied Biosystems STR kits, reagents, consumables, and GeneMapper ID-X Software, as well as industry-leading support.
The Applied Biosystems SeqStudio Genetic Analyzer for HID is a 4-capillary, easy-to-use, and convenient benchtop system that delivers gold-standard STR fragment analysis and Sanger sequencing with just one click. It is easily used across a broad range of forensic STR kits and applications. Get the same answers you trust with the outstanding data quality, service, and support you’ve come to expect from Applied Biosystems instruments.
The Applied Biosystems Precision ID NGS System for human identification can help you solve tough cases by getting more information from your challenging samples. By combining the Ion Chef System and either the Ion GeneStudio S5 next-generation sequencing systems with Applied Biosystems Precision ID panels that include forensically relevant markers, adopting NGS in your laboratory is now simpler than ever. The Precision ID mtDNA Whole Genome Panel, using the control region data for analysis, is approved for inclusion in the US National DNA Index System (NDIS) CODIS database.
Applied Biosystems GlobalFiler STR PCR amplification kits combine reduced amplification time with maximum discrimination power. As part of the only fully integrated and validated forensic workflow, this breakthrough 6-dye, 24-locus technology is designed to deliver superior lab performance so you can do more with less.
The GlobalFiler kit is also available with an Internal Quality Control system, or IQC, as part of a fully integrated and validated forensic workflow. The IQC system consists of two synthetic sequences with specific primers for each of the targets (IQCS and IQCL) and provides positive confirmation of sample amplification, but can also indicate adverse conditions with compromised amplification, such as the presence of PCR inhibitors. The IQC system, also used in our VeriFiler Plus and NGM Detect kits, provides additional confidence in genotyping results, and can help users distinguish, for example, between inhibited and degraded DNA samples.
The Applied Biosystems Yfiler Plus PCR Amplification Kit also leverages 6-Dye technology and contains 27 Y-STR markers that are valuable to use when evidence samples are difficult to resolve with traditional autosomal STR analysis, due to low amounts of male DNA present in a high background of female DNA.
The Precision ID GlobalFiler NGS STR Panels include the same 21 autosomal STRs along with Y markers and amelogenin sex markers found in the Applied Biosystems GlobalFiler PCR Amplification Kit. In place of SE33, the Precision ID GlobalFiler NGS STR Panels contain additional multiallellic STR markers, including Penta D and Penta E in the v2 panel for the Ion S5 System, to aid in mixture interpretation of complex casework samples.
The Precision ID GlobalFiler NGS STR Panel v2 is specifically designed for sequencing on the Ion S5 and Ion S5 XL Systems. Library preparation can be performed manually, on a robotic platform, or on the Ion Chef System, with template preparation optimized for the Ion Chef System.
The Precision ID GlobalFiler NGS STR Panel is currently available for use with manual library preparation, template preparation on the Ion Chef System or the Ion OneTouch 2 System, and sequencing only on the Ion PGM System.
The Applied Biosystems Precision ID mtDNA Whole Genome Panel uses an innovative approach to mitochondrial DNA sequencing specifically developed for forensic applications. This mtDNA tiling approach includes two primer pools of 81 small amplicons in each pool, with an average size of 163 bp to assist with obtaining optimal mitochondrial genome coverage data from highly compromised, degraded samples such as hair shafts, teeth, and bones.
Precision ID mtDNA Control Region Panel uses the same mtDNA tiling approach used in the Ion AmpliSeq mtDNA Whole Genome Panel. This targeted control region panel contains two smaller sets of primer pools with 7 amplicons each that span the 1.2 kb control region, which encompasses HV-I, II, and III.
The Applied Biosystems Precision ID Ancestry Panel includes 165 autosomal markers (SNPs) that can provide you with biogeographic ancestry information and guide your investigation process.
The Applied Biosystems Precision ID Identity Panel, comprising 124 markers, provides discrimination of individuals similar to STR genotype match probabilities used by forensic analysts.
Our Human Identification Professional Services (HPS) team has been helping customers like you successfully navigate the validation process required to bring new technologies into operation. At the core of our offerings are services designed to help forensic DNA labs integrate, validate, and implement new methodologies and technologies.
Human identification webinars
Learn more from our library of Human Identification Resources.
Unless stated otherwise on the individual product labeling, products referred to are For Research, Forensic, or Paternity Use Only. Not for use in diagnostic procedures.