DNA Quantification Kits
The Applied Biosystems Quantifiler HP DNA Quantification Kit enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human DNA in a single, highly sensitive real-time PCR reaction. This guides selection of the optimal short tandem repeat (STR) analysis chemistry (autosomal or miniSTR) and streamlines the workflow while increasing downstream analysis success rates.
The Quantifiler Trio kit has all the enhanced capabilities of Quantifiler HP, plus the addition of an improved male Y target for more sensitive male:female ratio detection and improved results in sexual assault casework.
HID RealTime PCR Analysis Software is designed for the RealTime PCR System and streamlines the experimental setup, supports customizable workflow templates, and enhances the data analysis and review process. The software is validated for use on both Windows XP and Windows 7 platforms, and is specialized for all Quantifiler kits on the market and optimized for the new Quantifiler Trio and the Quantifiler HP DNA Quantification Kits.
Key features and benefits
More informative and comprehensive casework sample assessment for improved downstream results
Increased workflow efficiency with faster time-to-results and flexible sample-processing protocols
Flexible protocols allow for configurable output and display features
Decision flow enhancement
The Quantifiler Trio and Quantifiler HP kits, used in conjunction with the HID Real-Time PCR Analysis Software, redefine the casework sample-processing workflow. New quality index functionality enables a more comprehensive view of sample quality (both degradation and inhibition), while increased sensitivity and robustness correlate with more recently released Applied Biosystems STR chemistries. This enables you to screen samples and make critical workflow decisions (for example, autosomal vs. Y-STR) with greater confidence.
The inclusion of quantity and quality indicators helps deliver accurate, sensitive, and specific results that work together with the software to enable informative, integrated workflow decisions.
Standards, targets, run method, and experiment properties are predefined for each Quantifiler template, with the flexibility to support custom assays.
Color-coded analysis summary of standards and samples, hyperlinked to flagged samples for quick data review.
Novel Y-screen assay for sexual assault samples using the Quantifiler Trio DNA Quantification Kit
by Allison Holt, Sheri Olson, Michele Marfori and Denise Yong Ning Oh
A. Holt, R. Green, J. Mulero, J. Shewale, J. Muehling
As labs worldwide are increasing the throughput of samples through their casework workflows, there is heightened interest in solutions that can increase productivity, maximize data output, and minimize errors.
With our workflow-centric design, we have incorporated robotic system requirements into our latest HID Real-Time PCR Analysis Software v1.2, which enables simple integration and upgrade to a fully optimized, automated quantification system to drive efficiency.
For labs that find it difficult to identify or implement the right robotic system(s) for their midstream workflow, our Human Identification Professional Services (HPS) team has extensive expertise in assisting in the design and implementation of robotic systems from a variety of vendors, and can partner with you to identify the most appropriate solution to meet your specific lab needs.
|Quantifiler Trio||Quantifiler HP||Quantifiler Duo||Quantifiler Human|
|Quantification targets||Total human
|Total human||Total human
|Sensitivity||<1 pg||<1 pg||~6 pg||~16 pg|
|Reaction mix and IPC robustness||Excellent||Excellent||Good||Good|
|Correlates with latest STR kits||Yes||Yes||No||No|
|Accuracy of M:F mixture ratio||Excellent||N/A||Very good||N/A|
|DNA quantification standard||Excellent||Excellent||Very good||Good|
|Cycling time||~60 min||~60 min||~90 min||~90 min|
|Minimum standards per plate||5||5||8||8|
|Fully integrated system with instrument, data collection, and analysis software||Yes||Yes||Yes||Yes|
|Validated real-time PCR instrument(s)||QuantStudio 5/7500||QuantStudio 5/7500||QuantStudio 5/7500||QuantStudio 5/7500|
|Compatible analysis software||HID Real-Time PCR Analysis Software v1,v1.2,v1.3||HID Real-Time PCR Analysis Software v1,v1.2,v1.3||HID Real-Time PCR Analysis Software v1.2,v1.3||
|Software with customized workflow templates, data analysis, and dilution/STR setup tools||HID Real-Time PCR Analysis Software v1,v1.2,v1.3||HID Real-Time PCR Analysis Software v1,v1.2,v1.3||HID Real-Time PCR Analysis Software v1.2,v1.3||
|Software with Windows® 7 compatibility and automation/LIMS integration capabilities||HID Real-Time PCR Analysis Software v1,v1.2,v1.3||HID Real-Time PCR Analysis Software v1,v1.2,v1.3||HID Real-Time PCR Analysis Software v1.2,v1.3||
|Order now||Order now||Order now||Order now|
For Research Use Only. Not for use in diagnostic procedures.