Each Quantifiler® Duo kit includes all reagents necessary for the amplification, detection, and quantification of 400 samples in 25 ul reactions using the Applied Biosystems 7500 Real-Time PCR System. Component Composition Quantifiler® Duo Primer Mix Primer pairs for amplification of RPPH1, SRY and IPC. The TaqMan® probes for RPPH1, SRY and IPC are labeled with VIC®, FAM™ and NED™ dyes, respectively. In addition, this reagent contains Internal PCR Control (IPC) template. Quantifiler® Duo PCR Reaction Mix AmpliTaq Gold® DNA Polymerase in buffer and salts Quantifiler® Duo DNA Standard Pooled human male genomic DNA Quantifiler® Duo Dilution Buffer 10 mM Tris HCl buffer, pH 8.0 containing 0.1 mM EDTA