GeneMapper® ID-X v1.5 Software Questions

As the newest version of our powerful genotyping software for forensic casework and reference sample data analysis, GeneMapper® ID-X v1.5 helps reduce manual effort in database uploading and offers better integration capabilities with third-party systems. Features include:

Feature Benefit
Microsoft® Windows® 7, 64-bit compatibility Supports popular IT upgrade
Automated key software operations Helps reduce time and effort needed to transfer data to laboratory information management systems (LIMS) through automated export of individual sample plot PDFs and ability to create and split projects.
Support CODIS CMF v3.2 (R13) specification Helps reduce manual labor and transcription errors between GeneMapper® ID-X and CODIS upload
Enhanced contamination assessment functionality Helps enable a robust reference check with less effort through the ability to import legacy reference profiles and export comparison results for further data analysis and reporting.


  • Incorporated GlobalFiler® Population Database

Recommended system configuration:

Intel® Core™ i3-3240 CPU @ 3.40GHz or newer


10 GB available on boot drive

DVD-ROM drive

10/100 NIC with RWU (internal) or above installed

TCP/IP installed

Supported operating systems:

  • Windows® 7 Professional, 64-bit (Service Pack 1)

Yes, GeneMapper® ID-X software can analyze .fsa and .hid files generated from all Applied Biosystems genetic analyzers, all AmpFℓSTR® and GlobalFiler® PCR Amplification Kits as well as projects created using previous versions of GeneMapper® ID-X Genotyping Software.

We encourage you to upgrade to v1.5 which offers improved efficiency and productivity. Please note that GeneMapper® ID v3.2, GeneMapper® ID-X v1.1, and 1.2 will be discontinued at the end of 2015.

General GeneMapper® ID-X software questions

By utilizing the automated, rule-based, subject-specific knowledge on which an expert system is based, much of the analysis process can be simplified, empowering you to make key interpretation decisions more quickly, confidently, and consistently. By leveraging the expert system functionality in GeneMapper® ID-X as well as the integrated mixture analysis tool, you can quickly assess controls and QC data, and easily review and edit single source, routine, and process mixed samples more efficiently, with maximum consistency and reproducibility.

The Mixture Analysis tool is fully integrated within the GeneMapper® ID-X software and is accessible via the Tools menu. The Mixture Analysis tool performs the following functions:

  1. Identifies samples within the GeneMapper® ID-X project that are eligible for Mixture Analysis. Samples are assessed according to sample type, analysis status, and allele label status.
  2. Categorizes and segregates eligible samples by the minimum number of contributors contained in each sample (1, 2, or 3 or more contributors).
  3. Extracts and separates contributor profiles into individual major and minor genotype combinations for all markers present. (2 contributors only.)
  4. Filters known profiles from selected sample profiles (2 contributors only).
  5. Calculates statistics using population databases stored within the software.

The Mixture Analysis tool extracts all possible genotype combinations from detected peaks at each locus and utilizes heterozygote peak height ratio and a measure of mixture proportion to evaluate each genotype combination.

You will be able to define the Mixture Interpretation Threshold (MIT) used to filter peaks which meet your criteria for mixture interpretation and the peak height ratio thresholds applied to peaks falling within specific peak height ranges. The residual value used to evaluate the fit to expected mixture proportion is set within the software and cannot be changed by the user. Mixture analysis methods may be defined that correspond to the interpretation criteria developed by your laboratory, based on your internal validation studies.

The GeneMapper® ID-X Software v1.5 Mixture Analysis tool only extracts individual contributors from mixtures containing 2 contributors, which represent a high proportion of the mixtures encountered by forensic laboratories. However, statistical calculations can be performed on mixtures containing 3 or more contributors.

The available statistical calculations depend on the number of contributors contained within the sample. The software will calculate the following:

  • Likelihood Ratio (LR): For samples categorized as containing 1 or 2 contributors
  • Random Match Probability (RMP): for samples categorized as single source and for each contributor extracted from a 2 person mixture
  • Combined Probability of Inclusion/Exclusion (CPI/CPE): for samples categorized as containing 2 and 3 or more contributors

Yes. Three population databases are provided with the GeneMapper® ID-X v1.5 software for use with the Mixture Analysis tool, but full instructions on how to create and import custom databases are provided in the GeneMapper® ID-X Software Help.

Comprehensive information regarding the functions performed in the Mixture Analysis tool are available in the GeneMapper®ID-X Software Help installed with the software. Two additional documents, the GeneMapper® ID-X Software v1.5 Getting Started Guide and the GeneMapper® ID-X Software v1.5 Quick Reference Guide will also be provided with the software. If you are interested in a demo version of the software, contact your account manager.

The number of clients connected per database could impact the speed of the system and is dependent on variables such as individual laboratory network performance and laboratory workflow. Initial evaluations performed by Applied Biosystems indicated no dramatic degradation in speed of operation when 96 samples were analyzed simultaneously by 10 clients connected to a single database. Each laboratory can use this as general guidance and determine the appropriate number of clients to ensure flexibility of operation, while maintaining an acceptable speed of operation for their particular laboratory environment.

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