Sexual assault kit (SAK) samples are among the most difficult sample types encountered by many forensic laboratories. Samples collected from female victims may contain very low amounts of male DNA mixed with high amounts of female DNA. It is critical to be able to quickly and accurately assess whether swab evidence from an SAK contains a male contributor before labor-intensive differential extraction procedures are performed.

Legislation requiring the submission of unsubmitted cases and specifying turnaround times will lead to an unprecedented increase in the number of SAKs submitted to laboratories, necessitating speedy processing to prevent backlogs.

Generate quality results with confidence

Our 6-dye chemistries of the Applied Biosystems™ QuantiFiler™ Trio, GlobalFiler™, and Yfiler™ Plus kits provide a compatible workflow to help maximize information generated on each sample, the first time through the workflow.  

Customer studies have demonstrated the ability of the QuantiFiler Trio DNA Quantification Kit to accurately predict degradation in downstream results using the GlobalFiler PCR Amplification kits. This allows laboratories to adjust input amounts to improve allele recovery. Accurate and sensitive detection of male DNA enabled by the QuantiFiler Trio allows the Yfiler Plus PCR Amplification Kit to be selected in cases of low male:female DNA ratio (M:F<1:20) where an autosomal kit would have little value. 


Featured products
QuantiFiler Trio

Better decision support on STR strategy
Learn more  


Highly discriminating chemistry, particularly for DNA mixture samples
Learn more ›

Yfiler Plus

Superior Y detection using rapidly mutating Y-STRs when male:female ratio is less than 1:20
Learn more ›

Sexual assault casework workflow
1 Screen for male DNA
Analyze Male DNA

Y-screen DNA assay using
Trio kit ›

Manual Slide Search

Serological Assays


2 Extract DNA

Differential extraction using the
Automate Express system ›

3 Quantify DNA


Low male? Degraded DNA? Inhibited?
Low concentration?

Informative decision support on STR strategy using
QuantiFiler Trio ›

4 Amplify DNA

3500 Genetic Analyizer

Use autosomal STR such as GlobalFiler kit ›

Select Y-STR analysis using Yfiler Plus kit ›

5 Analyze data
Laptop with DNA

Analyze results such as quality flags using GeneMapper ID-X v1.5 Software ›

Need help bringing products online?

Look no further than our Human Identification Professional Services (HPS) team. Since 2007, the team has completed over 400 successful validation projects worldwide. 

Over 20 technical support specialists, each averaging 8 years of real-world forensic experience, provide customers with in-depth training and support on our instruments, chemistries, and software.

Learn more

Webinar and resources

Franz Neuhuber

Watch the video to hear Dr. Franz Neuhuber, Head of Forensic Molecular Biology Department at University of Salzburg, Austria share details of an attempted rape case that prompted his lab to adopt Y-STR analysis.

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Customer support

If you have additional comments or questions, please contact the customer service team via email.

Technical support

If you are a current customer and need technical support, please contact the global service team via email, or phone us at 1-888-821-4HID (4443). Select option #1 for immediate assistance.