As part of the validation process, Applied Biosystems tested the performance of the PrepFiler™ kit against a standard organic Phenol/Chloroform process and 3 other commonly used commercially available chemistries on a variety of simulated forensic casework samples (prepared from 2 ul blood, 50 ul saliva, and 1 ul semen). The extracts were evaluated for DNA concentration, total quantity of DNA isolated, and purity using the Quantifiler Human DNA Quantification Kit. An example of the results is shown above.
In all cases, the PrepFiler™ kit matched or exceeded the concentration and total yield of DNA produced by other methods. For certain sample types, the PrepFiler™ kit yielded greater than 100% more DNA than the other technologies tested.