FAIMS Pro interface delivers maximum usability and selectivity for proteomics workflows.
Achieve the ultimate in LC-MS performance and ion mobility spectrometry when you utilize the tool-free, one-way assembly of Thermo Scientific FAIMS Pro interface, a field asymmetric ion mobility spectrometry (FAIMS) technique that seamlessly works with select Thermo Scientific mass spectrometers. Together they further enhance superior selectivity and enable greater proteome profiling while reducing time consuming sample preparation steps.
| Protect precious samples | Fast, higher quality results | Low cost and improved ease of operation | Focus on science, not setup |
|---|---|---|---|
| The FAIMS Pro technology is the only interface exclusively designed to improve nano, capillary, and microflow applications, allowing you to preserve precious, size-limited samples while achieving higher data quality. | The unique design of FAIMS Pro technology enhances instrument selectivity and detection limits using gas phase fractionation. This results in reduced matrix interference and higher quality data, faster. | Increased selectivity allows you to improve performance and save time by avoiding or reducing offline sample fractionation preparation steps. No consumable parts means lower long-term cost and maintenance. | Designed for inexperienced users to easily install and use. Leverage menu-driven software to design methods using pre-configured parameter recommendations and quickly achieve results. |
Brochure: Reveal what matters with field asymmetric ion mobility spectrometry
Effortless selectivity accelerates your proteomics workflow
The FAIMS Pro interface is a next-generation, differential ion mobility device that enhances performance and usability of our mass spectrometers to enable identification and quantitation of more proteins.
- Flexible to fit your work
- Easy to install, use, and maintain
- Increases coverage without extra work
- Conserves sample
How does the FAIMS Pro interface work?
The FAIMS Pro interface is easy to set up and deploy to enhance experimental performance and is perfectly suited for nano to micro flow applications (up to 25µL/min). Better selectivity means increased productivity for every proteomic user.
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Conventional versus differential ion mobility spectrometry (FAIMS)
Identifying and characterizing proteins and post-translational modifications by bottom-up mass spectrometry relies on the acquisition of high quality MS and MS/MS data. The FAIMS Pro interface increases analytical performance through gas phase fractionation and selective enhancement of peptidic compounds, reducing the complexity of the MS spectra, and improving analyte signal/noise ratio. The end result is greater proteome coverage.
OTIT -140 min gradient
Comparison of protein and peptide identifications using conventional setup (no FAIMS) or FAIMS with 3 CVs (-50, -65, and -85 V). The addition of the FAIMS Pro interface enables an improvement of 14% in protein IDs and 19% in peptide IDs when compared to not using FAIMS in DDA mode, with a 90-minute nanoflow gradient on the Orbitrap Fusion Lumos MS.
A loading curve of HeLa lysate digest with a 90-minute nanoflow gradient and no FAIMS Pro interface compared to FAIMS (CVs at -50 and -70 V) shows improvement in peptide IDs ranging between 25-73% on an Orbitrap Fusion Lumos MS.
Free webinar: Enhanced proteome analysis with FAIMS-MS
In this session with Dr. Michael Westphall from University of Wisconsin, Madison, learn why ion mobility isn’t what you’ve been missing for high throughput proteomics. When Dr. Westphall connected the FAIMS Pro interface with our LCMS systems it enabled him to obtain up to a 20% or more increase in the number of proteins and peptides detected.
Related software to enhance data acquisition and processing
Through accessing Xcalibur software revision 3.1 or greater and Proteome Discoverer software revision 2.3 or greater, you can automate tuning and optimization, leverage data-acquisition method template recommendations and streamline data processing making setup and experimental success to results easier.
Download Xcalibur now Try Proteome Discoverer now
Learn more about our Multi-Omics Data Analysis options.
