Break Free Sample LC-MS sample complexity
Maximum usability and selectivity for a broad range of mass spectrometry workflows

Maximum usability and selectivity for a broad range of mass spectrometry workflows

The Thermo Scientific FAIMS Pro Duo interface extends differential ion mobility to address analytical challenges for a broad range of qualitative and quantitative applications. Increase signal-to-noise ratios, sample throughput, and coverage for large- and small-molecule LC-MSn methods. Enhanced method optimization lets you easily integrate orthogonal selectivity into existing workflows.


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Key benefits of the FAIMS Pro Duo interface
Experimental flexibilityFast, higher-quality resultsLow cost and improved ease of operationFocus on science, not setup
The FAIMS Pro Duo interface technology is the only interface exclusively designed to improve applications covering a wide range of chromatographic flow rates on different compound types analyzed on a range of Thermo Scientific next-generation mass spectrometers.The unique design of the FAIMS Pro Duo interface technology enhances instrument selectivity and detection limits using gas-phase fractionation. This results in reduced matrix interference and higher-quality data, faster.Increased selectivity allows you to improve performance and save time by avoiding or reducing offline sample fractionation preparation steps. No consumable parts means lower long-term cost and maintenance.Designed for inexperienced users to easily install and use. Leverage online CV optimizaiton routines and menu-driven software to design methods and quickly achieve results.


How does the FAIMS Pro Duo interface work?

The FAIMS Pro Duo interface is easy to set up and deploy to enhance experimental performance and is perfectly suited for nano-to-analytical flow-rate applications (up to 1000µL/min). Better selectivity means increased productivity for every user.

See how FAIMS Pro interface works
Expanded view of electrodes and entrance plate assembly.

Conventional versus differential ion mobility spectrometry (FAIMS)

Identifying and characterizing proteins and post-translational modifications by bottom-up mass spectrometry relies on the acquisition of high-quality MS and MS/MS data. The FAIMS Pro Duo interface increases analytical performance through gas-phase fractionation and selective enhancement of peptidic compounds, reducing the complexity of the MS spectra, and improving analyte signal/noise ratio. The end result is greater proteome coverage.

Proteome analysis using the FAIMS Pro Duo interface increases proteome coverage for both low and high sample loading amounts. Comparative analysis of the number of proteins and peptides confidently identified in an analysis of a tryptic digest of HeLa cell lysate under identical chromatographic conditions. While the proteome coverage measured using the standard Data-dependent Acquisition (DDA) method at low and high sample loading amounts is impressive, simply incorporating the FAIMS Pro Duo interface substantially increased protein and peptide coverage with almost 8,000 proteins confidently measured in the 140-minute method.

Incorporation of the FAIMS Pro Duo interface into an existing DDA experimental workflow increases proteome profiling efficiency. Compared to using a standard DDA method, the FAIMS Pro Duo interface method provided similar proteome coverage while using substantially less sample and shorter chromatographic gradients, addressing the two primary concerns for translational proteomics. Comparison of protein and peptide identifications using conventional setup (control) or FAIMS Pro Duo interface (FAIMS) with 3 CVs (-50, -65, and -85 V). The addition of the FAIMS Pro Duo interface enables an improvement of 18% in protein IDs and 29% in peptide IDs when compared to not using FAIMS in DDA mode, with a 140-minute nanoflow gradient on the Thermo Scientific Orbitrap Fusion Lumos Tribrid mass spectrometer.



Featured videos

Dr. Devin Schweppe of Harvard Medical School discusses the Thermo Scientific FAIMS Pro interface for improved quantitative accuracy and peptide quantitation.

Scott Peterman discusses the Thermo Scientific FAIMS Pro interface for improved quantitative accuracy and maximum ion transmission and proteome coverage.


FAIMS Pro Duo interface product & ordering guide

Through accessing Thermo Scientific Xcalibur software revision 3.1 or greater and Thermo Scientific Proteome Discoverer software revision 2.3 or greater, you can automate tuning and optimization, leverage data-acquisition method template recommendations and streamline data processing, making setup and experimental success to results easier.

Learn more about our Multi-Omics Data Analysis options.


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