Orbitrap Tribrid LC-MS New Customer Center

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Explore the information below and discover the capabilities of your new instrument, or please contact us to speak with a specialist if there is something we can help you with.

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Mass spectrometry
rewards program

Get rewarded for your new Orbitrap Tribrid MS with savings up to 40% off select Thermo Scientific MS products.

Calibration solutions,
standards and solvents

Ready-to-use liquid formulations that can quickly calibrate Thermo Scientific LC-MS instrumentation.



Data analysis

Thermo Scientific Proteome Discoverer software simplifies a wide range of proteomics workflows and is recommended for quantitative analysis of TMT-based data. The software has improved data processing capabilities for handling large data sets and is enabled with study management tools.

Unless stated otherwise, Proteome Discoverer software would be recommended for each of the workflows below.
The below workflows are not relevant for the Thermo Scientific Orbitrap ID-X Tribrid Mass Spectrometer.

Amine-reactive Thermo Scientific TMT10plex Isobaric Mass Tag Labeling Reagent Set plus TMT11-131C increases sample multiplexing from 10-plex to 11-plex, enabling even greater throughput for protein identification and quantitative analysis by tandem mass spectrometry (MS).

Sample labeling for TMT-based quantitation

Protein extracts isolated from cells or tissues are reduced, alkylated and digested overnight. Samples are labeled with the TMT Reagents and then mixed before sample fractionation and clean-up. Labeled samples are analyzed by high resolution Orbitrap LC-MS/MS to identify peptides and quantify reporter ion relative abundance. See the
 User's Guide for a step-by-step TMT labeling procedure for intact proteins as well as peptides including preparing whole cell protein extracts, protein digestion, and peptide labeling.

Free webinar: Sample prep reagents for improved proteome depth, quantification and throughput

Simplifying Complexity: New MS Reagents that Improve Proteome Depth, Quantification, and Throughput

This webinar focuses on an optimized proteomics sample preparation workflow for isobaric tags and highlights new tools for fractionation, sample throughput, accurate quantitation.

Trapping column

Separator column

Amine-reactive Thermo Scientific TMT10plex Isobaric Mass Tag Labeling Reagent Set plus TMT11-131C increases sample multiplexing from 10-plex to 11-plex, enabling even greater throughput for protein identification and quantitative analysis by tandem mass spectrometry (MS).

Sample labeling for TMT-based quantitation

Protein extracts isolated from cells or tissues are reduced, alkylated and digested overnight. Samples are labeled with the TMT Reagents and then mixed before sample fractionation and clean-up. Labeled samples are analyzed by high resolution Orbitrap LC-MS/MS to identify peptides and quantify reporter ion relative abundance. See the
 User's Guide for a step-by-step TMT labeling procedure for intact proteins as well as peptides including preparing whole cell protein extracts, protein digestion, and peptide labeling.

Free webinar: Sample prep reagents for improved proteome depth, quantification and throughput

Simplifying Complexity: New MS Reagents that Improve Proteome Depth, Quantification, and Throughput

This webinar focuses on an optimized proteomics sample preparation workflow for isobaric tags and highlights new tools for fractionation, sample throughput, accurate quantitation.

Trapping column

Separator column

Mass spec analysis of TMT labeled samples

What is synchronous precursor selection?

The SPS method begins with selection of the parent ion in the MS scan, followed by its isolation in the quadrupole and fragmentation by collisionally induced dissociation (CID) in the ion trap. Following fragmentation, SPS enables simultaneous isolation of up to 20 MS2 fragment ions. A select group of MS2 fragment ions are then transferred back into the ion routing multipole (IRM) where they undergo higher energy collisional dissociation (HCD) fragmentation, with the MS3 fragments then detected in the Orbitrap analyzer.

The use of SPS dramatically increases the reporter ion signal intensity and improves the ratio accuracy, due to improved counting statistics, leading to a significant increase in the number of quantified peptides.

Watch this free webinar to learn more

Robust classification of stem cell protein location by SPS-MS 3

Kathryn Lilley from University of Cambridge describes hyperLOPIT; a spatial proteomics method which enables the characterization of protein subcellular localization.

Technical resources

Technology

Phosphopeptide and TMT

Cancer proteomics

Other applications

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Thermo Scientific Pierce Trypsin/Lys-C protease mix, MS-Grade, is a mass spectrometry (MS)-grade serine endoproteinase mixture of trypsin and LysC that can be used for concurrent digestion of proteins for more efficient digestion than with trypsin alone.

The Thermo Scientific High-Select TiO2 Phosphopeptide Enrichment Kit enables efficient enrichment of phosphorylated peptides from complex and fractionated protein digests for analysis by mass spectrometry (MS). (This product replaces Cat. No. 88301.)

The Thermo Scientific High-Select Fe-NTA Phosphopeptide Enrichment Kit enables efficient enrichment of phosphorylated peptides from complex and fractionated protein digests for analysis by mass spectrometry (MS). (This product replaces Cat. No. 88300.)

Thermo Scientific Pierce Trypsin/Lys-C protease mix, MS-Grade, is a mass spectrometry (MS)-grade serine endoproteinase mixture of trypsin and LysC that can be used for concurrent digestion of proteins for more efficient digestion than with trypsin alone.

The Thermo Scientific High-Select TiO2 Phosphopeptide Enrichment Kit enables efficient enrichment of phosphorylated peptides from complex and fractionated protein digests for analysis by mass spectrometry (MS). (This product replaces Cat. No. 88301.)

The Thermo Scientific High-Select Fe-NTA Phosphopeptide Enrichment Kit enables efficient enrichment of phosphorylated peptides from complex and fractionated protein digests for analysis by mass spectrometry (MS). (This product replaces Cat. No. 88300.)

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After size-exclusion chromatography to separate the crosslinked complex, digest using MS grade proteolytic enzyme.

Simplifying complexity: New MS reagents that improve proteome depth, quantification, and throughput

This webinar focuses on an optimized proteomics sample preparation workflow for isobaric tags and highlights new tools for fractionation, sample throughput, accurate quantitation.

After size-exclusion chromatography to separate the crosslinked complex, digest using MS grade proteolytic enzyme.

Simplifying complexity: New MS reagents that improve proteome depth, quantification, and throughput

This webinar focuses on an optimized proteomics sample preparation workflow for isobaric tags and highlights new tools for fractionation, sample throughput, accurate quantitation.

Technical resources

General

Top

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<li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/CMD/posters/PN-64761-Recombinant-Proteins-ASMS2016-PN64761-EN.pdf" target="_blank">Poster Note: Development of Mass Spectrometry-Based Methods for Quality Assessment of Recombinant Proteins</a></li>

 

<li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/CMD/posters/PN-64761-Recombinant-Proteins-ASMS2016-PN64761-EN.pdf" target="_blank">Poster Note: Development of Mass Spectrometry-Based Methods for Quality Assessment of Recombinant Proteins</a></li>

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* Only available for Thermo Scientific Orbitrap Eclipse Tribrid Mass Spectrometer.
* Only available for Thermo Scientific Orbitrap Eclipse Tribrid Mass Spectrometer.

Technical resources

Top

PepSwift Monolithic Capillary LC Columns are based on a polystyrene divinylbenzene copolymer, the monolithic structure offers a high-quality alternative to traditional microparticulate sorbents, providing important advantages to the chromatographic separation. High-sensitivity proteomics and biotech applications are easily performed using these columns.

Ensure robust nano and capillary flow LC-MS with EASY-Spray LC Columns. The integrated column/emitter design eliminates dead volume and is temperature-controlled for maximum reliability and performance. Rigorously tested to ensure maximum quality, these columns are designed for maximum simplicity and ease-of-use.

MAbPac RP columns for high-performance reversed-phase (RP) HPLC and LC/MS characterization of monoclonal antibodies (mAbs), fragments, variants, antibody drug conjugates (ADCs), PEGylated proteins, and bispecific proteins. These columns have excellent resolution, long column lifetime, and low carry-over. The surface chemistry, particle size, and pore size, have been optimized for high resolution separations.

PepSwift Monolithic Capillary LC Columns are based on a polystyrene divinylbenzene copolymer, the monolithic structure offers a high-quality alternative to traditional microparticulate sorbents, providing important advantages to the chromatographic separation. High-sensitivity proteomics and biotech applications are easily performed using these columns.

Ensure robust nano and capillary flow LC-MS with EASY-Spray LC Columns. The integrated column/emitter design eliminates dead volume and is temperature-controlled for maximum reliability and performance. Rigorously tested to ensure maximum quality, these columns are designed for maximum simplicity and ease-of-use.

MAbPac RP columns for high-performance reversed-phase (RP) HPLC and LC/MS characterization of monoclonal antibodies (mAbs), fragments, variants, antibody drug conjugates (ADCs), PEGylated proteins, and bispecific proteins. These columns have excellent resolution, long column lifetime, and low carry-over. The surface chemistry, particle size, and pore size, have been optimized for high resolution separations.

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Achieve exceptional peak shape and resolution for your HPLC and LC/MS applications with Hypersil GOLD HPLC columns. These endcapped, ultrapure, silica-based columns deliver significant reduction in peak tailing using generic gradients with C18 selectivity. With their excellent resolution, efficiency, and sensitivity, Hypersil GOLD columns give you confidence in the accuracy and quality of your analytical data.

Achieve exceptional peak shape and resolution for your HPLC and LC/MS applications with Hypersil GOLD HPLC columns. These endcapped, ultrapure, silica-based columns deliver significant reduction in peak tailing using generic gradients with C18 selectivity. With their excellent resolution, efficiency, and sensitivity, Hypersil GOLD columns give you confidence in the accuracy and quality of your analytical data.

MAbPac SEC-1 size exclusion chromatography HPLC columns for high-resolution separation of monoclonal antibody (mAb) analysis and large proteins, These size-exclusion chromatography (SEC) columns provide superior performance, even under nondenaturing conditions, and in high- and low-salt mobile phases and volatile eluents.

MAbPac SCX-10 LC Columns for high-resolution, high efficiency analysis of monoclonal antibodies (mAbs) and associated variants. The unique nonporous pellicular resin provides the resolving power to separate mAb variants that differ by as little as one charged residue. A hydrophilic layer surrounds the polymeric beads and eliminates hydrophobic interactions with the resin, resulting in very efficient peaks. A proprietary grafted cation-exchange surface provides pH selectivity control for high-resolution separations.

MAbPac SEC-1 size exclusion chromatography HPLC columns for high-resolution separation of monoclonal antibody (mAb) analysis and large proteins, These size-exclusion chromatography (SEC) columns provide superior performance, even under nondenaturing conditions, and in high- and low-salt mobile phases and volatile eluents.

MAbPac SCX-10 LC Columns for high-resolution, high efficiency analysis of monoclonal antibodies (mAbs) and associated variants. The unique nonporous pellicular resin provides the resolving power to separate mAb variants that differ by as little as one charged residue. A hydrophilic layer surrounds the polymeric beads and eliminates hydrophobic interactions with the resin, resulting in very efficient peaks. A proprietary grafted cation-exchange surface provides pH selectivity control for high-resolution separations.


CMD SchemaApp code