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Orbitrap Exploris Mass Spectrometers
Fast track to confident results
Thermo Scientific Orbitrap Exploris mass spectrometry is quantitative high-resolution, accurate-mass (HRAM) liquid chromatography mass spectrometry (LC-MS) with record-setting performance for your next-generation laboratory. The power of built-in intelligence, sensitivity and selectivity delivers depth of analysis to the lowest levels with high quantitative accuracy and precision. This extraordinary power is now available in an expanded portfolio to meet your exact analytical requirements. Scale up your research and translate your results more rapidly and simply than ever before with Orbitrap Exploris mass spectrometry.
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The evolution of 20 years of Orbitrap technology
Perfect
Fit
Orbitrap Exploris MX mass detector
Accelerate biopharma commercialization, manufacturing and quality control by easily and reliably obtaining compliance-ready, high-quality mass information.
Productivity redefined
Orbitrap Exploris 120 MS
Consistent and accurate data, operational simplicity, instrument ruggedness for method development and everyday testing.
Leading Performance
Orbitrap Exploris 240 MS
Precision and accuracy to drive discovery and identification, confidently scale up and achieve impact, with application versatility and operational simplicity.
Extraordinary Simplified
Orbitrap Exploris 480 MS
Next-level insights, exploratory and targeted quantitation, next-gen power, performance, and ease of use for proteomics and biopharma applications.
Why choose an Orbitrap Exploris mass spectrometer for your lab?
Maximize your return on investment in a unified Thermo Scientific mass spectrometry architecture from data acquisition and analysis solutions
Video series: Orbitrap Exploris MS - Ease of use
Which Orbitrap Exploris MS is best suited for your lab?
The Orbitrap Exploris mass spectrometry systems offer a variety of performance features designed to address everyday research to routine testing demands and ideal for addressing mass spectral analysis of low-level components in complex matrices applications. Find the best system to design your next-generation laboratory capabilities and meet your changing business needs for more efficient screening (including targeted, suspected and nontargeted), identification, confirmation and quantitation.
 |  |  |  | |
| Thermo Scientific Orbitrap Exploris MX mass detector | Thermo Scientific Orbitrap Exploris 120 mass spectrometer | Thermo Scientific Orbitrap Exploris 240 mass spectrometer | Thermo Scientific Orbitrap Exploris 480 mass spectrometer | |
|---|---|---|---|---|
| Analyses | Targeted / non-targeted screening (MS) Unknown Identification (MS) Absolute and Relative Quantitation (MS) | Targeted / non-targeted screening (MS) Unknown Identification (MS) Confirmation (MS/MS) Absolute and Relative Quantitation (MS and/or MS2) | ||
| Ideal applications |
| |||
| Advanced Data-Dependent Experiments | N/A | N/A | Precursor Fit | SureQuant, Precursor Fit, Boxcar Scanning, TurboTMT |
| Next generation ion source interface | Yes | |||
| Quick start methods for various applications | Yes | |||
| Drag-n-drop method setup user interface | Yes | |||
| Almanac Web-Based Monitoring and Management | Yes | Requires Foundation 3.1 SP1 or later | ||
| Automated background exclusion | N/A | N/A | Requires Foundation 3.1 SP1 or later | |
| Standard Features | ||||
| Max Resolution (FWHM) @ m/z 200 | 180,000 | 120,000 | 240,000 | 480,000 |
| Mass Accuracy (Internal Calibration) | <1 ppm for up to 5 days with EASY-IC ion source | |||
| Calibration procedures | Thermo Scientific Pierce FlexMix Calibration solution | |||
| Dynamic Range | >5,000 within a single Orbitrap mass analyzer spectrum | Up to 5 orders | ||
| Mass Range | m/z 40–3,000 up to m/z 8,000 with BioPharma option | m/z 40 – 3,000 | m/z 40 - 6000 (up to 8000 with BioPharma option) | |
| Scan Speed | Up to 22 Hz | Up to 40 Hz | ||
| Polarity Switching | 1.4 Hz | |||
| Ion Source interface | OptaMax NG ion source | |||
| Multiplexing | N/A | Up to 20 precursors/scan | ||
| Scan Functions | Unique scan types are available
| Unique scan types are available
| Unique scan types are available
| Unique scan types are available
|
| Dissociation | In-source CID | In-source CID, HCD | ||
| Options and/or Upgrades | ||||
| Intact Protein Mode | Included with BioPharma option | N/A | Yes | |
| High Mass Range (HMR, up to 8000 m/z) | Yes, with BioPharma option | N/A | Yes | |
| Sensitivity | Full Scan: 200 fg reserpine on column S/N 150:1 | MS/MS: 200 fg reserpine on column S/N 100:1 SIM: 200 fg reserpine on column S/N 250:1 | MS/MS: 50 fg reserpine on column S/N 100:1 SIM: 50 fg reserpine on column S/N 150:1 | |
| Chromatography system recommendations | Vanquish Flex HPLC system Vanquish Horizon HPLC system | Vanquish Core HPLC system Vanquish Flex HPLC system Vanquish Duo HPLC system Transcend Multichannel system | Vanquish Flex HPLC system Vanquish Duo HPLC system Vanquish Horizon HPLC system Vanquish Neo system | |
| Sources | OptaMax NG ion source FAIMS Pro Duo Interface EASY-IC ion source | APCI/APPI Combination Ion Source | FAIMS Pro Duo interface APCI/APPI Combination Ion Source EASY-Spray Sources Nanospray Flex Ion Sources | |
| Externally triggered sources | N/A | AP-SMALDI5 AF source for MS Imaging | AP-SMALDI5 AF source for MS Imaging | AP-SMALDI5 AF source for MS Imaging |
What’s inside the Orbitrap Exploris mass spectrometer
The highly optimized design of Orbitrap Exploris mass spectrometers permits a compact physical footprint while providing high performance components and many new functionalities that advance performance for critical applications. The design allows us to achieve a more compact footprint with a single turbo-pump with six-stage pumping, thus fewer moving parts, less electricity consumption, noise and weight.
Ion path and key components of Orbitrap Exploris 120 and Orbitrap Exploris 240 mass spectrometers
TransMIT Center for Mass Spectrometric Development (TransMIT GmbH, Giessen, Germany) offers integrated high-end solutions for atmospheric-pressure Scanning microprobe Matrix-Assisted Laser Desorption/Ionization (MALDI) mass spectrometric imaging. These solutions are based on TransMIT’s proprietary SMALDI MS Imaging and 3D-Surface MS Imaging technology. The AP-SMALDI source generates ions under atmospheric pressure (AP) and the experimental setup allows to systematically scan tissue samples or 3D objects with the pulsed laser beam applying laser spot sizes of 5 μm routinely and without oversampling.
Due to its high performance in spatial resolution,TransMIT’s AP-SMALDI5 AF ion source is the perfect match for the high mass resolution and accurate mass capabilities of Orbitrap MS instrumentation.
AP-SMALDI MS imaging of healthy mouse brain tissue using the Orbitrap Exploris 240 mass spectrometer with three m/z values extracted in blue, red and green. Compounds extracted are location-specific endogenous metabolites of the mouse brain tissue. 2D pixel mode of acquisition with 40 µm x 40 µm pixels size and with 5 µm laser spot size applied. Full MS scan from m/z 600 to m/z 1000.
The coupling of AP-SMALDI5 AF and Orbitrap technologies enables high mass and spatial resolution by providing:
- Automated matrix application with crystal size below 5 µm, via TransMIT's SMALDIPrep device
- Lateral and spatial resolution of 5 µm and MS Imaging acquisition modes with choices for highest acquisition speed (2D Line Mode) or highest sensitivity (Full Pixel Mode)
- Automated laser focusing to investigate non-flat surfaces and 3-dimensional surfaces (3D-Surface MS Imaging)
- TransMIT’s SMALDIControl Software to control ion source, laser, x,y,z-stage and mass spectrometer
- Orbitrap Exploris MS HRAM instrumentation supported by its Instrument Control Software (Tune and Method Editor application) supporting the capabilities to trigger the mass spectrometer by an external device suited for MS Imaging applications
- TransMIT’s Mirion MS Image generation software with full imzML integration for post-processing MS imaging raw data information
Schematic diagram of TransMIT’s AP-SMALDI5 AF ion source mounted to the Orbitrap Exploris platform.
AP-SMALDI ion source schematic detail: (1) the laser beam passes through a focusing lens (2) two mirrors directing the light through an objective lens (3) close to the sample. The objective lens (3) focuses the pulsed laser light to the tissue sample base plate (4). An off-axis camera (5) allows for sample inspection while the autofocusing (6) laser ensures the analyzing laser is always in focus. Technical details of the AP-SMALDI5 AF setup coupled to Orbitrap mass spectrometry for MS Imaging application. Note: AP-SMALDI5 AF ion source is a laser class 1 product.
TransMIT’s SMALDIPrep device, its AP-SMALDI5 AF ion source, its Mirion MS Image generation software and Orbitrap Exploris MS instrumentation allows for spatial multi-omics analysis from tissue and from surfaces of 3D objects.
Drug imaging and uptake kinetics in Fasciola hepatica parasite samples using AP-SMALDI MSI
AP-SMALDI MS Imaging enabled by Orbitrap technology allows to successfully track anti-Fasciola compounds during their penetration into parasitic tissues upon time course studies.
High resolution in mass and space: AP-SMALDI coupled with Orbitrap Exploris mass spectrometer for MS Imaging
High resolution in mass and space is achieved by Atmospheric-Pressure Scanning microprobe Matrix-Assisted Laser Desorption/Ionization (AP-SMALDI) coupled with Thermo Scientific Orbitrap mass spectrometers.
TransMIT's SMALDIControl software controls TransMIT's AP-SMALDI AF ion source coupled with Thermo Scientific Orbitrap Exploris instrumentation.
TransMIT's MIRION software generates MS images acquired by TransMIT's AP-SMALDI AF ion source coupled with Thermo Scientific Orbitrap Exploris instrumentation.
Dr Bernhard Spengler of TransMIT Center discusses 3D imaging with AP-SMALDI5 AF technology when coupled with Thermo Scientific Orbitrap Exploris mass spectrometers
Orbitrap Exploris mass spectrometer ordering information
Video: Compounding Insights—How to extract high confidence understanding from comprehensive Orbitrap Exploris HRAM data
Laboratories performing LC-MS applications strive for certainty when identifying detected compounds. For many small-molecule applications, conventional approaches aren’t enough. Laboratories require more, such as rich, high quality spectral information and fit for purpose data analysis software to make satisfactory identifications and characterization.
Thermo Scientific Compound Discoverer software helps you extract high-confidence understanding from comprehensive Orbitrap high-resolution, accurate-mass data.
The Lab-Forward Trade-In Program
The world needs science to move faster. Now more than ever. Which is why when more scientists have access to the latest scientific instrumentation, we all benefit. That is the sole purpose of our Lab-Forward Trade-In Program. For a limited time, trade in your chromatography and mass spectrometry instruments and save!
Successful Grant-Writing Starts Here
7 steps to your grant-writing success
A well-thought-out and detailed grant proposal is essential. Our technical experts are with you every step of the way in the grant-writing process to help you reach new heights.
Smart Note: How does AcquireX intelligent data acquisition improve laboratory efficiency
Acquiring high-quality data doesn’t need to be a challenge
Your samples contain vast amounts of information, which provides both knowledge and value. Learn how The Thermo Scientific AcquireX intelligent data acquisition workflow enables you to access this information by acquiring the highest quality, in-depth LC-MSn data possible.
White paper: Challenges and emerging directions in single-cell proteomics
Learn about the challenges of single-cell proteomics compared to single-cell genomics and transcriptions. Review new technologies and methods that address these challenges. Find out more about improvements that include automation, miniaturized sample preparation, multiplexing, and fast, robust LC-MS protocols, plus consider the outlook for single-cell proteomics.
Application Note: Spatial multi-omics at the cellular level by AP-SMALDI MS imaging
The goal of the experiments described is to characterize small metabolites, lipids and tryptic peptides in complex tissue architectures at the low-micrometer scale by means of spatial resolution when applying AP-SMALDI 5 AF technique coupled to Thermo Scientific™ Orbitrap Exploris™ mass spectrometry.
The results represent a comprehensive spatial multi-omics study—metabolomics, lipidomics, and proteomics—at the cellular level of mouse kidney by means of mass spectrometry imaging.
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