Orbitrap Exploris Mass Spectrometers

Fast track to confident results

Thermo Scientific Orbitrap Exploris mass spectrometry is quantitative high-resolution, accurate-mass (HRAM) liquid chromatography mass spectrometry (LC-MS) with record-setting performance for your next-generation laboratory. The power of built-in intelligence, sensitivity and selectivity delivers depth of analysis to the lowest levels with high quantitative accuracy and precision. This extraordinary power is now available in an expanded portfolio to meet your exact analytical requirements. Scale up your research and translate your results more rapidly and simply than ever before with Orbitrap Exploris mass spectrometry.


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The evolution of 20 years of Orbitrap technology

Productivity redefined

Orbitrap Exploris 120 mass spectrometer

Consistent and accurate data, operational simplicity, instrument ruggedness for method development and everyday testing.

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Leading Performance

Orbitrap Exploris 240 mass spectrometer

Precision and accuracy to drive discovery, scale up and achieve impact, with application versatility and operational simplicity.


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Extraordinary Simplified

Orbitrap Exploris 480 mass spectrometer

Next-level insights, exploratory and targeted quantitation, next-gen power, performance, and ease of use for proteomics and biopharma applications.

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Why choose an Orbitrap Exploris mass spectrometer for your lab?

Market leading resolution for uncompromising data certainty

The combination of high-resolution, mass accuracy and stability ensures you have the highest level of spectral quality to help simplify your most complex analytical challenges.

Day-to-day performance consistency

Set it and forget it for days of maximum mass accuracy with built-in internal system calibration.

Out-of-the-box simplicity

Intuitive tuning, single-click calibration, drag-and-drop method setup, and pre-defined experiments driven by next-generation software are designed to help you get to reportable results easier, every day, for all users.

Improve lab operations with a compact footprint

Overcome space challenges even though your lab may be experiencing growth in sample volume, while still delivering maximum business value with the MS performance you need and expect, without compromising quality of results.


Maximize your return on investment in a unified Thermo Scientific mass spectrometry architecture from data acquisition and analysis solutions


Which Orbitrap Exploris MS is best suited for your lab?

The Orbitrap Exploris mass spectrometry systems offer a variety of performance features designed to address everyday research to routine testing demands and ideal for addressing mass spectral analysis of low-level components in complex matrices applications. Find the best system to design your next-generation laboratory capabilities and meet your changing business needs for more efficient screening (including targeted, suspected and nontargeted), identification, confirmation and quantitation.

 Orbitrap Exploris 120Orbitrap Exploris 240Orbitrap Exploris 480
 Thermo Scientific
Orbitrap Exploris 120
mass spectrometer
Thermo Scientific
Orbitrap Exploris 240
mass spectrometer
Thermo Scientific
Orbitrap Exploris 480
mass spectrometer
AnalysesTargeted/non-targeted screening (MS)
Unknown Identification (MS)
Confirmation (MS/MS)
Absolute and Relative Quantitation (MS and/or MS2)
Ideal applications
Advanced Data-Dependent ExperimentsN/APrecursor FitSureQuant, Precursor Fit, Boxcar Scanning
Next generation ion source interface Yes
Quick start methods for various applicationsYes
Drag-n-drop method setup user interfaceYes
Almanac Web-Based Monitoring and ManagementRequires Foundation 3.1 SP1 or later
Automated background exclusionN/ARequires Foundation 3.1 SP1 or later
Standard Features
Max Resolution (FWHM) @ m/z 200120,000240,000480,000
Mass Accuracy (Internal Calibration)<1 ppm for up to 5 days with EASY-IC ion source
Calibration proceduresThermo Scientific Pierce FlexMix Calibration solution
Dynamic RangeUp to 5 orders
Mass Rangem/z 40 – 3,000m/z 40 - 6000
(up to 8000 with BioPharma option)
Scan SpeedUp to 22 HzUp to 40 Hz
Polarity Switching1.4 Hz
Ion Source interfaceOptaMax NG ion source
MultiplexingUp to 20 precursors/scan
Scan FunctionsUnique scan types are available
  • Full MS
  • AIF
  • t-SIM
  • DIA
  • MS2
and combinable within in one single experiment, such as:
  • Full Scan ddMS2 (Top 1-4)
  • tSIM (targeted mass list) ddMS2 (Top 1-4)
  • Full Scan ddMS2 (targeted mass list) (Top 1-4)
In addition, multiple experiments can be created combining all of the above listed scan types.
Unique scan types are available
  • Full MS
  • AIF
  • SIM
  • DIA
  • MS2
and combinable within in one single experiment, such as:
  • Full Scan ddMS2 (TopN)
  • Full Scan ddSIM
  • tSIM (targeted mass list) ddMS2
  • Full Scan ddMS2 (targeted mass list) (TopN)
All above with options for
  • ‘Number of Scans’ (= TopN)
  • ‘Cycle Time’
In addition, multiple experiments can be created combining all of the above listed scan types.
Unique scan types are available
  • Full MS
  • AIF
  • SIM
  • DIA
  • MS2
and combinable within in one single experiment, such as:
  • Full Scan ddMS2
  • Full Scan ddSIM
  • tSIM (targeted mass list) ddMS2
  • Full Scan ddMS2 (targeted mass list)
All above with options for
  • ‘Number of Scans’ (= TopN)
  • ‘Cycle Time’
  • ‘Scans per Outcome’ (branching)
In addition, multiple experiments can be created combining all of the above listed scan types.
DissociationIn-source CID, HCD
Options and/or Upgrades
Intact Protein ModeN/AYes
High Mass Range (HMR, up to 8000 m/z)N/AYes
SensitivityMS/MS: 200 fg reserpine on column S/N 100:1
SIM: 200 fg reserpine on column S/N 250:1
MS/MS: 50 fg reserpine on column S/N 100:1
SIM: 50 fg reserpine on column S/N 150:1
Chromatography system recommendationsVanquish Core HPLC system
Vanquish Flex HPLC system
Vanquish Duo HPLC system
Transcend Multichannel system
Vanquish Flex HPLC system
Vanquish Duo HPLC system
Vanquish Horizon HPLC system
EASY-nLC 1200 system
UltiMate 3000 RSLCnano system
SourcesAPCI/APPI Combination Ion SourceFAIMS Pro Duo Interface
APCI/APPI Combination Ion Source
EASY-Spray Sources
Nanospray Flex Ion Sources

What’s inside the Orbitrap Exploris mass spectrometer

The highly optimized design of Orbitrap Exploris mass spectrometers permits a compact physical footprint while providing high performance components and many new functionalities that advance performance for critical applications. The design allows us to achieve a more compact footprint with a single turbo-pump with six-stage pumping, thus fewer moving parts, less electricity consumption, noise and weight.

Ion path and key components of Orbitrap Exploris 120 and Orbitrap Exploris 240 mass spectrometers

Ion path and key components of an Orbitrap Exploris Mass Spectrometer

Orbitrap Exploris mass spectrometer ordering information


Video: Compounding Insights—How to extract high confidence understanding from comprehensive Orbitrap Exploris HRAM data

Laboratories performing LC-MS applications strive for certainty when identifying detected compounds. For many small-molecule applications, conventional approaches aren’t enough. Laboratories require more, such as rich, high quality spectral information and fit for purpose data analysis software to make satisfactory identifications and characterization.

Thermo Scientific Compound Discoverer software helps you extract high-confidence understanding from comprehensive Orbitrap high-resolution, accurate-mass data.


Smart Note: How does AcquireX intelligent data acquisition improve laboratory efficiency

White paper: Challenges and emerging directions in single-cell proteomics


Resources

CMD SchemaApp code