Scale up your science. With new capabilities for multiplexed quantitative proteomics and native protein characterization, the Thermo Scientific Orbitrap Ascend Tribrid mass spectrometer delivers ultimate experimental throughput, versatility, and ease-of-use. Quantify more samples at lower concentrations using intelligent acquisition. Achieve greater coverage using a revolutionary new hardware design featuring dual ion routing multipoles. Or characterize the largest biopharmaceuticals with optional mass range up to m/z 16,000. All while producing more with less instrument setup utilizing the Auto-Ready ion source for automated calibration, and pre-built, optimized method templates to maximize convenience and ease-of-use. Be ready for tomorrow’s most challenging demands.

Highlights of Orbitrap Ascend Tribrid mass spectrometer

Improved detection, versatility, and sensitivity for protein complex subunit analysis and biopharmaceutical characterization with an extended optional mass range up to m/z 16,000.

Demonstration of 16,000 m/z mass range on purified GroEL protein on a single Orbitrap scan

Demonstration of m/z 16,000  mass range on purified GroEL 800 kDa protein on a single Orbitrap scan at 7500 resolution at m/z 200. Critical core functionalities include AGC controlled ion measurements compatible with advanced ion manipulation techniques including: ETD, PTCR, UVPD, and CID/HCD.

Confidently identify more of the proteome with dual ion routing multiples to increase scan rate for proteomic applications. Quantify more samples with TMTpro 18plex multiplexing using intelligent acquisition with faster scanning data acquisition for higher sample throughput, single cell proteomics, and post-translational modifications (PTMs).

Improved phosphopeptide analysis
Spectral Quality

Improved phosphopeptide analysis Ti-IMAC beads were added to tryptic peptides for enrichment. 500ng was injected and analyzed using a 90 min gradient using Orbitrap data-dependent methods. Sample courtesy of Yuchen He, Coon Laboratories, University of Wisconsin-Madison.

Quantify up to 18 samples with coverage to >8,000 proteins in <9 hours
Quantify up to 18 samples with coverage to >8,000 proteins in <9 hours

Human cells were labeled with TMTpro 18plex reagents, combined, fractionated, and subsequently 12 high pH fractions were analyzed using 75 min gradient with SPS MS3 Real-Time Search methods. The Orbitrap Ascend Tribrid MS was able to achieve the sample depth of proteome coverage in 30% less time than the Orbitrap Eclipse Tribrid MS. Sample courtesy of Steven Shuken, Qing Yu, and Steven Gygi, Harvard Medical School.

Optimized method templates and ready-to-use instrument control software are designed to help you quickly profile your complex samples. An automated instrument calibration and dedicated Auto-Ready ion source hardware ensure the instrument is ready when you are for data acquisition.

Integrated Auto-Ready ion source

Spend less time on instrument set-up with the integrated Auto-Ready ion source. Trigger on-demand or scheduled system calibrations remotely and without disruption to your LC-MS system.

Instrument hardware design

The proven Tribrid architecture combines the best quadrupole, linear ion trap, and Orbitrap HRAM technologies to acquire the richest MS data for every sample run. Multiple fragmentation techniques – CID and HCD with optional UVPD, ETD, PCTR – are available at any stage of MS, with subsequent mass analysis in either the ion trap or ultra-high resolution Orbitrap mass analyzer.

The Orbitrap Ascend Tribrid MS ion path
 Click image to enlarge

Orbitrap Ascend Tribrid MS ion path

The Orbitrap Ascend Tribrid MS combines industry-leading versatility and usability with the exceptional performance of its revolutionary Tribrid architecture to deliver an instrument to address the most difficult analytical challenges.

Single Cell Sensitivity

Empowers the next generation to explore new applications and reveal cellular diversity.

Intelligent acquisition to collect more meaningful data

A comprehensive, integrated set of data analysis tools and automated data processing enable you to confidently and quickly produce information-rich MSn data on complex samples.

Application-specific methods just one click away

The intuitive method editor features a drag-and-drop user-friendly interface with pre-built, optimized method templates for a wide range of applications. This allows scientists to generate high-quality data easily, such as single cell proteomics, PTMs, TMT, SureQuant methods, intact protein analysis, metabolomics, lipidomics, and more. The common method editor combines both instrument control and data acquisition parameters, allowing you to acquire high-quality data from the start.

Monitor your mass spectrometry analyses anywhere, anytime.

Monitor your mass spectrometry analyses anywhere, anytime

Using the Thermo Scientific Almanac web- and smartphone-based application gives you the ability to remotely access and manage your LC-MS systems, freeing you to focus on other important tasks during your day.

Check real-time system status and acquisitions, set up automated e-mails to notify you of a completed acquisition or error, schedule instrument access, monitor utilization, or send service files to aid in system diagnostics and maximize up-time.

Key Almanac tools

  • Instrument Connect Platform: manage your systems and data in a single location
  • Instrument Status: see what is happening in real time
  • Instrument Logbook and Events: easily see what has happened and log important information
  • Instrument Utilization Reports: evaluate system usage over time
  • Instrument Scheduler: manage system access and utilization
  • Push to Service: minimize time taken to diagnose issues
Almanac Workflow

Connect your laboratory: Always know what’s going on within your laboratory with any cloud-connected device. Maximize system utilization with long-term trending of system usage and sample throughput, through to checking data acquisition and multiple other productivity tools.

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Proteomics research requires more than just simple identification. Thermo Scientific Proteome Discoverer software offers a full suite of analysis tools with the flexibility to address multiple research workflows, and an easy-to-use, wizard-driven interface. Confidently interpret your data with sophisticated statistical and visualization tools unique to Proteome Discoverer software, and utilize third-party algorithms to take your analysis to the next level. Go beyond searching to discovering, with intelligent INFERYS™ Rescoring and the novel CHIMERYS™ intelligent search algorithm by MSAID. These innovative approaches substantially increase the identification rate and the number of unique peptide identifications.

Recommended for the following workflows:

  • Top-down proteomics
  • Label-free quantitation
  • Cross-linking
  • Glycopeptide identification and quantification
  • Phosphopeptide ID and quantification
  • Iterative search strategies
  • Multi-search engine comparison
  • INFERYS Rescoring and CHIMERYS intelligent search algorithm by MSAID.

Complete protein characterization can be challenging, whether you are performing intact protein mass analysis, top- and middle-down analysis, peptide mapping or multi-attribute method (MAM) workflows. Access workflows to facilitate comprehensive interpretation and data visualization, confidently characterizing your biologics with speed and ease. Thermo Scientific BioPharma Finder software helps you choose the right path for confident protein characterization.

Recommended for the following workflows:

  • Intact analysis
  • Top-down and middle-down analysis
  • Peptide mapping
  • Multi-attribute method (MAM)
  • Comprehensive oligonucleotide analysis capabilities
Joshua Coon, PhD Principal Investigator, Coon Laboratories, University of Wisconsin-Madison.
"One thing on the Orbitrap Ascend Tribrid MS that is really exciting is the new ion routing multiples, and how by moving the collision cell forward, the speed of the system has gotten even faster from the last model. That means that we can dig deeper in a sample, and we can detect more things in a shorter period of time.”
Joshua Coon, PhD Principal Investigator, Coon Laboratories, University of Wisconsin-Madison.




Hardware specifications

Thermo Scientific OptaMax NG ion source

Features sprayer with improved alignment and stability, interchangeable HESI/APCI probe, and an exhaust port design that efficiently removes solvent vapor, to reduce chemical noise and improve uptime.

Auto-Ready ion source

  • Integrates source with dedicated emitter and ion transfer tube to simplify calibration and maintenance.
  • Internal Thermo Scientific Pierce FlexMix Calibration solution consumable lasts ≥3 months with regular use.

Electrodynamic Ion Funnel

A modified ion funnel captures the ions under more gentle conditions, improving the transfer of labile compounds while maintaining optimal ion transmission

Advanced Active Ion Beam Guide

Advanced active ion beam guide with an axial field and low-pass filter prevents neutrals and high-velocity clusters from entering the quadrupole mass filter

QR5 Quadrupole mass filter

Next generation quadrupole mass filter with 5.25 mm field radius delivers industry leading ion transmission across m/z 40–2000 range for efficient precursor selection with m/z 0.4–1200 isolation width

Dual Ion-routing multipoles

  • Ion storage capabilities
  • Higher-energy collisional dissociation (HCD)
  • Variable pressure control from 0.5 to 20 mTorr with HMRn+ option, for small and large molecule workflows
  • Efficient and reliable ion transfer between the ion-routing multipole, Orbitrap mass analyzer and linear ion trap mass analyzer

Orbitrap mass analyzer

  • Next-generation ultra-high-field Orbitrap mass analyzer
  • Low noise detection pre-amplifier
  • Automatic calibration of all ion transfer parameters

Dual-pressure linear ion trap

  • Extended front section of the high-pressure cell for improved ETD and PTCR reaction control
  • For precursors between m/z 50 and 1800, isolation widths range from m/z 0.2 to 3000
  • Collision-induced dissociation (CID)
  • ETD/EThcD/ETciD and UVPD fragmentation techniques
  • PTCR ion-ion reaction

Vacuum system

  • Split-flow turbomolecular pump provides vacuum in three regions
  • Enhanced vacuum technology reduces the pressure in the ultra-high vacuum region to <10–10 Torr
  • Improved Orbitrap mass analyzer performance at a wider range of collision gas settings

Optional hardware

Ultraviolet Photodissociation (UVPD) ion source

Includes a Class 1, 213 nm laser system with 2.5 kHz repetition rate, delivering >1.2 μJ per pulse.

EASY-ETD ion source

  • Generates fluoranthene anions for ETD
  • Improved dynamic range and signal-to-noise ratio (S/N) for fragments using high dynamic range EASY-ETD HD

PTCR ion source

  • Extension of the EASY-ETD ion source
  • Generates perfluoroperhydrophenanthrene (PFPP) ions for subsequent gas-phase ion-ion reactions
  • Simplifies interpretation of complex spectra by decreasing the charge state of precursors and/or product ions

EASY-IC ion source

  • Generates internal calibrant ions for real-time mass calibration of spectra in positive and negative modes
  • Provides <1 ppm RMS drift over 24 hours, measured with Flex Mix Calibration Solution

High mass range with MSn+(HMRn+)

  • Extends the mass range to m/z 16,000 for detection of precursor and product ions in the Orbitrap mass analyzer
  • Precursor ion isolation up to m/z 8000 using the linear ion trap

FAIMS Pro Duo interface

Performs online gas-phase separation based on differential ion mobility for orthogonal separation and selectivity. Optimizes performance for 100 nL/min to 1 mL/min flow rates.


Mass range

Standard mass range m/z 40–2000, mid-mass range m/z 200–6000, and optional HMRn+ mass range m/z 500–16,000

Orbitrap mass analyzer resolution

Standard resolution settings range from 7500 to 480,000 (FWHM) at m/z 200

Acquisition rate (under defined conditions)

Orbitrap mass analyzer MSⁿ up to 45 Hz.
Ion trap MSⁿ up to 50 Hz.

Mass accuracy (under defined conditions)

<3 ppm RMS using external calibration.
<1 ppm RMS using internal calibration with EASY-IC source.

MS/MS electrospray ionization (ESI) ion trap sensitivity

2 μL of a 50 fg/μL solution of reserpine (100 femtograms total) injected at a flow rate of 500 μL/min produces a minimum S/N ratio of 200:1 for the transition of the isolated protonated molecular ions at m/z 609 to the largest two product ions, m/z 397 and m/z 448, when the mass spectrometer is operated at unit resolution in the full scanMS/MS mode

Dynamic range

>5,000 within a single MS acquisition.

Multiplexing using ion-routing multipole

Up to 20 precursors can be multiplexed using the quadrupole mass filter and ion-routing multipoles to perform selected ion monitoring or tandem MS 

Polarity switching

One full experimental cycle acquired in <1.1 seconds where the cycle consists of acquiring one full MS scan in positive and negative polarity at a resolution setting of 30,000

Installation requirements


230 Vac ±10% single phase, 50/60 Hz, with ground, 3× outlets at 15 A each


  • Ultra-high purity Helium (99.999%) with less than 1 ppm each of water, oxygen, and total hydrocarbons
  • High purity Nitrogen (99.5% pure, flow rate 15 L/min) gas supply for the API source and ion routing multipole (IRM)

Dimensions (WxDxH)

1270 × 805 × 703 mm (50 × 31.7 × 27.7 in)


180 kg (400 lb) without data system, vacuum rough pumps and optional items


Operating environment must be 16–26 °C (59–78 °F) and relative humidity must be 50–80% with no condensation

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7 steps to your grant-writing success

A well-thought-out and detailed grant proposal is essential. Our technical experts are with you every step of the way in the grant-writing process to help you reach new heights.

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Spend less time on sample prep, and more on proteomics research

The Thermo Scientific AccelerOme automated sample preparation platform, with factory-supplied reagents and kits, intuitive software and a unique experimental design experience transforms proteomics sample preparation.

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