Orbitrap Ascend Tribrid
Mass Spectrometer
 

Specifications

Attribute

Description

Hardware specifications

Thermo Scientific OptaMax NG ion source

Features sprayer with improved alignment and stability, interchangeable HESI/APCI probe, and an exhaust port design that efficiently removes solvent vapor, to reduce chemical noise and improve uptime.

Auto-Ready ion source

• Integrates source with dedicated emitter and ion transfer tube to simplify calibration and maintenance.
• Internal Thermo Scientific Pierce FlexMix Calibration solution consumable lasts ≥3 months with regular use.

Electrodynamic Ion Funnel

A modified ion funnel captures the ions under more gentle conditions, improving the transfer of labile compounds while maintaining optimal ion transmission

Advanced Active Ion Beam Guide

Advanced active ion beam guide with an axial field and low-pass filter prevents neutrals and high-velocity clusters from entering the quadrupole mass filter

QR5 Quadrupole mass filter

Next generation quadrupole mass filter with 5.25 mm field radius delivers industry leading ion transmission across m/z 40–2000 range for efficient precursor selection with m/z 0.4–1200 isolation width

Dual Ion-routing multipoles

• Ion storage capabilities
• Higher-energy collisional dissociation (HCD)
• Variable pressure control from 0.5 to 20 mTorr with HMRn+ option, for small and large molecule workflows
• Efficient and reliable ion transfer between the ion-routing multipole, Orbitrap mass analyzer and linear ion trap mass analyzer

Orbitrap mass analyzer

• Next-generation ultra-high-field Orbitrap mass analyzer
• Low noise detection pre-amplifier
• Automatic calibration of all ion transfer parameters

Dual-pressure linear ion trap

• Extended front section of the high-pressure cell for improved ETD and PTCR reaction control
• For precursors between m/z 50 and 1800, isolation widths range from m/z 0.2 to 3000
• Collision-induced dissociation (CID)
• ETD/EThcD/ETciD and UVPD fragmentation techniques
• PTCR ion-ion reaction

Vacuum system

• Split-flow turbomolecular pump provides vacuum in three regions
• Enhanced vacuum technology reduces the pressure in the ultra-high vacuum region to <10–10 Torr
• Improved Orbitrap mass analyzer performance at a wider range of collision gas settings

Optional hardware

Ultraviolet Photodissociation (UVPD) ion source

Includes a Class 1, 213 nm laser system with 2.5 kHz repetition rate, delivering >1.2 μJ per pulse.

EASY-ETD ion source

• Generates fluoranthene anions for ETD
• Improved dynamic range and signal-to-noise ratio (S/N) for fragments using high dynamic range EASY-ETD HD

PTCR ion source

• Extension of the EASY-ETD ion source
• Generates perfluoroperhydrophenanthrene (PFPP) ions for subsequent gas-phase ion-ion reactions
• Simplifies interpretation of complex spectra by decreasing the charge state of precursors and/or product ions

EASY-IC ion source

• Generates internal calibrant ions for real-time mass calibration of spectra in positive and negative modes
• Provides <1 ppm RMS drift over 24 hours, measured with Flex Mix Calibration Solution

High mass range with MSn+(HMRn+)

• Extends the mass range to m/z 16,000 for detection of precursor and product ions in the Orbitrap mass analyzer
• Precursor ion isolation up to m/z 8000 using the linear ion trap

FAIMS Pro Duo interface

Performs online gas-phase separation based on differential ion mobility for orthogonal separation and selectivity. Optimizes performance for 100 nL/min to 1 mL/min flow rates.

Performance

Mass range

Standard mass range m/z 40–2000, mid-mass range m/z 200–6000, and optional HMRn+ mass range m/z 500–16,000

Orbitrap mass analyzer resolution

Standard resolution settings range from 7500 to 480,000 (FWHM) at m/z 200

Acquisition rate (under defined conditions)

Orbitrap mass analyzer MSⁿ up to 45 Hz.
Ion trap MSⁿ up to 50 Hz.

Mass accuracy (under defined conditions)

<3 ppm RMS using external calibration.
<1 ppm RMS using internal calibration with EASY-IC source.

MS/MS electrospray ionization (ESI) ion trap sensitivity

2 μL of a 50 fg/μL solution of reserpine (100 femtograms total) injected at a flow rate of 500 μL/min produces a minimum S/N ratio of 200:1 for the transition of the isolated protonated molecular ions at m/z 609 to the largest two product ions, m/z 397 and m/z 448, when the mass spectrometer is operated at unit resolution in the full scanMS/MS mode

Dynamic range

>5,000 within a single MS acquisition.

Multiplexing using ion-routing multipole

Up to 20 precursors can be multiplexed using the quadrupole mass filter and ion-routing multipoles to perform selected ion monitoring or tandem MS 

Polarity switching

One full experimental cycle acquired in <1.1 seconds where the cycle consists of acquiring one full MS scan in positive and negative polarity at a resolution setting of 30,000

Installation requirements

Power

230 Vac ±10% single phase, 50/60 Hz, with ground, 3× outlets at 15 A each

Gas

• Ultra-high purity Helium (99.999%) with less than 1 ppm each of water, oxygen, and total hydrocarbons
• High purity Nitrogen (99.5% pure, flow rate 15 L/min) gas supply for the API source and ion routing multipole (IRM)

Dimensions (WxDxH)

1270 × 805 × 703 mm (50 × 31.7 × 27.7 in)

Weight

180 kg (400 lb) without data system, vacuum rough pumps and optional items

Environment

Operating environment must be 16–26 °C (59–78 °F) and relative humidity must be 50–80% with no condensation