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Affinity Purification Mass Spectrometry
Dynamic view of protein-protein interactions
Enrichment strategies such as affinity purification can be coupled to qualitative and quantitative mass spectrometry workflows to examine a subset of proteins in complex samples. Affinity enrichment makes use of specific binding interactions between molecules to isolate proteins of interest. In this approach, affinity purification mass spectrometry can be used to examine specific protein-protein interactions within protein complexes or to look at protein complexes more globally at the interactome level using the proximity biotinylation approach. By coupling affinity purification to quantitative MS, interactions can be studied under different conditions thereby providing much more dynamic view of protein-protein interactions. Furthermore, this workflow is amenable for PTM analysis which makes it possible to examine the role post-translational modifications (PTMs) play in facilitating protein-protein interactions.
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Our general purpose product lines, including LC-MS products, are not intended for in vitro diagnostic purposes in accordance with our product documentation, manuals, and labels. They are designated for General Laboratory Use Only.
Our general purpose product lines have not been tested or validated for such applications and their use for in vitro diagnostic purposes may result in health and safety risks. They are designated for Research Use Only Applications.
For Research Use Only - Not For Use In Diagnostic Procedures.
The Thermo Scientific Orbitrap Exploris and Excedion series mass spectrometers are Orbitrap instruments with an atmospheric pressure ionization (API) source for liquid chromatography (LC) mass spectrometry (MS) high-throughput applications. The instruments are designed to be placed on a bench in the laboratory and maintain mass accuracy across chromatographic peaks, supporting confident identification and quantitation using high-resolution accurate-mass detection.