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See beyond structure: HDX-MS for protein dynamics
Gain quantitative insights into solvent accessibility, backbone flexibility, and allosteric changes across proteins and protein complexes. Probe protein structure and conformational dynamics, and characterizing protein-protein and protein-ligand interactions under native-like conditions with hydrogen-deuterium exchange mass spectrometry (HDX-MS).
Uptake patterns can reveal conformational changes, map binding interface, and define dynamic regions that are invisible to static structural methods. By adding a dynamic perspective, HDX-MS data can guide and validate molecular dynamic (MD) simulations and complement structures from cryo-EM and X-ray.
Our mass spectrometers deliver high spectral quality for MS and MS/MS scans, supporting confident peptide identification and robust quantitation of deuterium incorporation. Together with integrated workflow solutions and advanced data analysis, HDX‑MS empowers scientists to build a comprehensive view of protein structure, motion, and interaction networks, effectively bridging the gap between protein structures and dynamics.
For Research Use Only. Not for use in diagnostic procedures.
General Laboratory Equipment – Not For Diagnostic Procedures. © 2026 Thermo Fisher Scientific Inc. All rights reserved. Thermo Scientific™ products are designated as General Laboratory Equipment and are not intended for use in diagnostic procedures or for in vitro diagnostic purposes. These products are intended for general laboratory use only and have not been tested or validated for diagnostic applications; use for such purposes may result in inaccurate results and may pose health and safety risks. They are designed for research, academic, and industrial applications and for general-purpose analytical use, including high-resolution and accurate-mass measurements, without reference to specific clinical conditions, analytes, or diagnostic decisions.
The Thermo Scientific Orbitrap Exploris and Excedion series mass spectrometers are Orbitrap instruments with an atmospheric pressure ionization (API) source for liquid chromatography (LC) mass spectrometry (MS) high-throughput applications. The instruments are designed to be placed on a bench in the laboratory and maintain mass accuracy across chromatographic peaks, supporting confident identification and quantitation using high-resolution accurate-mass detection.