Proteomics Mass Spectrometry Analysis

Comprehensive and confident proteomics analysis

Identifying peptides from complex mixtures with high-accuracy and high-precision quantification requires a combination of speed, high mass resolution accurate mass (HRAM), and multiple fragmentation techniques. The Thermo Scientific Orbitrap Fusion Lumos Tribrid, Q Exactive, and Triple Stage Quadrupole (TSQ) instrument lines easily perform qualitative and quantitative analysis of complex proteomics samples via ion isolation for MS/MS fragmentation.

In order to identify as many peptides as possible from complex mixtures and achieve both high accuracy and high precision during quantification, a combination of speed, high resolution accurate mass (HRAM), and multiple fragmentation techniques is needed. All available Orbitrap-based platforms capable of ion isolation for MS/MS fragmentation, including the Thermo Scientific Orbitrap Fusion Lumos, the Orbitrap Fusion, and the Q Exactive line of instruments, can perform qualitatitive and quantitative analysis of complex proteomics samples.

Targeted quantitation is frequently performed on triple quadrupole mass spectrometers such as the Thermo Scientific TSQ family, but can also be accomplished using the HRAM and MS/MS capabilities of the Orbitrap-based mass spectrometers. Using the same type of mass spectrometers for discovery and targeted quantitation experiments can simplify the transition to targeted quantitation methods. Thermo Scientific Orbitrap-based mass spectrometers can simultaneously quantify and confirm identification of multiple targeted proteins and peptides even in complex biological matrices.

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Featured peptide detection, quantitation and characterization categories

Find the power to increase productivity

Empowering life science researchers to translate each step to a new level of insight with a next generation ownership experience coupled with an intelligence-driven experimental approach. With the Thermo Scientific Orbitrap Exploris 480 mass spectrometer you'll find market leading quantitative performance to solve your most complex challenges with ease, and the proven qualitative performance you expect.

From exploratory to targeted quantitation, find the power and reliability to make large scale studies possible, while reducing everyday hassles as you increase productivity. And achieving it all, remarkably, within a compact footprint. An extraordinary instrument, providing extraordinary results.

Go beyond today's discovery

Today’s cutting-edge research pushes LC-MS to its limits. Obtaining high confidence insights to enable accurate resolution of subtle differences and avoid costly dead-ends is needed faster than ever before. The Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer surpasses these limits with new innovations that deliver the ultimate flexibility to expand experimental scope, and with built-in intelligence to ensure the highest data quality and confidence. One system provides maximum insights, so you productively go beyond today’s discovery.

Reduce sample interference, not system efficiency

Targeted MS, and in particular selected reaction monitoring (SRM), is the quantitative analysis method of choice on the Thermo Scientific TSQ Altis Triple Quadrupole Mass Spectrometer, enabling monitoring of up to 250 peptide-specific precursor pairs (precursors and product ions) per second while using 0.2-Dalton resolution for precursor ion selection. This dramatically reduces chemical interferences while maintaining high transmission efficiency.