Comprehensive and confident proteomics analysis
Identifying peptides from complex mixtures with high-accuracy and high-precision quantification requires a combination of speed, high mass resolution accurate mass (HRAM), and multiple fragmentation techniques. The Thermo Scientific Orbitrap Fusion Lumos Tribrid, Q Exactive, and Triple Stage Quadrupole (TSQ) instrument lines easily perform qualitative and quantitative analysis of complex proteomics samples via ion isolation for MS/MS fragmentation.
In order to identify as many peptides as possible from complex mixtures and achieve both high accuracy and high precision during quantification, a combination of speed, high resolution accurate mass (HRAM), and multiple fragmentation techniques is needed. All available Orbitrap-based platforms capable of ion isolation for MS/MS fragmentation, including the Thermo Scientific Orbitrap Fusion Lumos, the Orbitrap Fusion, and the Q Exactive line of instruments, can perform qualitatitive and quantitative analysis of complex proteomics samples.
Targeted quantitation is frequently performed on triple quadrupole mass spectrometers such as the Thermo Scientific TSQ family, but can also be accomplished using the HRAM and MS/MS capabilities of the Orbitrap-based mass spectrometers. Using the same type of mass spectrometers for discovery and targeted quantitation experiments can simplify the transition to targeted quantitation methods. Thermo Scientific Orbitrap-based mass spectrometers can simultaneously quantify and confirm identification of multiple targeted proteins and peptides even in complex biological matrices.
Featured peptide detection, quantitation and characterization categories
The Thermo Scientific Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer combines an HRAM ultra-high-field Orbitrap analyzer with high-performance quadrupole precursor ion selection to deliver unsurpassed speed while maintaining sensitivity over a wide dynamic range. Compared to previous Q Exactive generations, the Q Exactive HF MS offers 1.8x higher resolving power at the same transient length and 2x higher scan speed at almost the same resolution setting (15,000 vs 17,500 at m/z 200). This enables a 2x shorter analysis time for approximately the same number of unique peptide identifications.
The Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer offers the highest resolving power of all Orbitrap-based instruments (up to 500,000 at m/z 200), achieving unparalleled detection limits and selectivity in full-scan MS. The system's brighter ion source and Advanced Quadrupole Technology enable low limits of detection in complex matrices, while its Universal Method, powered by ADAPT technology, ensures optimal results from samples with unknown concentrations. Multiple fragmentation techniques (CID, HCD, ETD and EThcD) provide complementary fragment information for maximal sequence coverage and PTM characterization.
Targeted MS, and in particular selected reaction monitoring (SRM), is the quantitative analysis method of choice on the Thermo Scientific TSQ Altis Triple Quadrupole Mass Spectrometer, enabling monitoring of up to 250 peptide-specific precursor pairs (precursors and product ions) per second while using 0.2-Dalton resolution for precursor ion selection. This dramatically reduces chemical interferences while maintaining high transmission efficiency.