Crosslinking Mass Spectrometry

Understanding protein interactions

Crosslinking mass spectrometry analyzes protein-protein interactions that are “locked in place” to better understand how proteins affect biological processes such as signaling cascades, gene upregulation, and energy (ATP) production.

A majority of protein functions are determined by their interactions with other proteins and cellular components such as nucleic and fatty acids. It is via such interactions that biological processes commence, conclude, and change.

Therefore, the study of protein interactions using crosslinking mass spectrometry is instrumental in understanding both healthy and disease states.

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Studying protein function through interaction

Crosslinking mass spectrometry has emerged as a powerful technique for studying protein-protein interactions. During the process of crosslinking, chemical crosslinkers are used to chemically join components of interacting complexes. This is followed by liquid chromatography (LC) separation and identification by mass spectrometry (MS) analysis. This workflow enables the study of protein-protein interactions by maintaining the original interacting complexes.


Crosslinking analysis solutions
Powerful crosslinking data analysis

Thermo Scientific Proteome Discoverer Software offers a powerful means for analyzing crosslinking data: The new XlinkX node within the software features a fully integrated crosslink peptide search engine for crosslinking MS analysis. This node enables crosslinked peptide annotation with assignment of inter- and intra-crosslinked peptides and mono-adducts. It is also compatible with non-cleavable and MS-cleavable crosslinkers.


Crosslinking mass spectrometry workflow