Hydrogen Deuterium Exchange (HDX)

Robust method for full structural characterization

Hydrogen deuterium exchange (HDX) mass spectrometry (MS) is a powerful tool for studying the dynamics of higher order structure of protein-based therapeutics. The rate of hydrogen-to-deuterium exchange within the amide hydrogen on the backbone of biotherapeutics provides solvent accessibility information and thus protein structure and conformation can be inferred.

Orbitrap-based HDX MS using the Thermo Scientific Orbitrap Fusion Lumos Tribrid MS offers a robust method for the analysis of protein conformation, conformation dynamics, and protein-protein interactions.

Full structural characterization is critical in biopharmaceutical development where the subtle but critical local conformational changes can impact safety and efficacy.

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Popular hydrogen deuterium exchange (HDX) products

Hydrogen deuterium exchange (HDX) workflow

Hydrogen deuterium exchange (HDX) workflow
Any Orbitrap-based system can be coupled to the H/D-X PAL/LC front end

Hydrogen deuterium exchange (HDX) categories

Simplify a wide range of workflowsfrom protein and peptide identification to PTM analysisusing Thermo Scientific Proteome Discoverer software. The software supports multiple database search algorithms (SEQUEST, Z-Core, Mascot, etc.) and multiple dissociation techniques (CID, HCD, ETD) for more comprehensive analyses. 

SGS webinar

SGS webinar – Application of HDX-MS for drug discovery and development

Application of HDX-MS for drug discovery and development

In this webinar, theory and practice of HDX-MS is discussed in conjunction with the topics of pharmaceutical and biopharmaceutical industry.