Hydrogen Deuterium Exchange (HDX)

Robust method for full structural characterization

Hydrogen deuterium exchange (HDX) mass spectrometry (MS) is a powerful tool for studying the dynamics of higher order structure of protein-based therapeutics. The rate of hydrogen-to-deuterium exchange within the amide hydrogen on the backbone of biotherapeutics provides solvent accessibility information and thus protein structure and conformation can be inferred.

Orbitrap-based HDX MS using the Thermo Scientific Orbitrap Eclipse Tribrid MS offers a robust method for the analysis of protein conformation, conformation dynamics, and protein-protein interactions.

Full structural characterization is critical in biopharmaceutical development where the subtle but critical local conformational changes can impact safety and efficacy.

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Hydrogen deuterium exchange (HDX) workflow

Hydrogen deuterium exchange (HDX) workflow
Any Orbitrap-based system can be coupled to the H/D-X PAL/LC front end

Hydrogen deuterium exchange (HDX) categories

Test new limits of detection, characterization, and quantitation using the Thermo Scientific Orbitrap Eclipse Tribrid Mass Spectrometer. This latest Tribrid MS was designed to surpass performance in advanced biopharma. The instrument features enhanced sensitivity resulting in improved analyte detection, characterization, and quantitation, enabling scientists to perform more comprehensive sample analyses faster and with better accuracy than ever before.

Save time and identify more with Thermo Scientific BioPharma Finder integrated software. It provides an automated workflow for peptide identification, disulfide bond mapping, and quantitation of post-translational modifications (PTMs). BioPharma Finder software automates previously time-consuming manual processes, processes complex data, and integrates the results into concise, informative reports. 

Simplify a wide range of workflowsfrom protein and peptide identification to PTM analysisusing Thermo Scientific Proteome Discoverer software. The software supports multiple database search algorithms (SEQUEST, Z-Core, Mascot, etc.) and multiple dissociation techniques (CID, HCD, ETD) for more comprehensive analyses. 

   View the video: Characterization of Monoclonal Antibodies by HDX-MS

Resources

  • Pfizer & BioAnalytix Webinar - Improved biotherapeutic
    characterization using HDX-Mass Spectrometry
  • Characterization of Monoclonal Antibodies by HDX-MS

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