Confident protein characterization methods

Get the latest information on the analysis of biotherapeutic proteins such as monoclonal antibodies (mAbs) in their intact form. UHPLC is ideal for identifying modifications such as charge variants, as well as higher order structures through aggregation. Use our broad range of leading column chemistries for these and other intact analyses by liquid chromatography (LC). For rapid and precise accurate mass determination, try our uniquely powerful Thermo Scientific Orbitrap-based mass spectrometers (MS) for analysis of both denatured and native intact proteins.

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NEW intact protein chromatography built for biopharma

New Vanquish Flex UHPLC delivers confidence, with a fully bio-compatible flow path and proven compliance. Achieve unprecedented performance in retention time stability, sensitivity and separation efficiency with the widest range of column chemistries for biotherapeutic protein characterization. Demand versatility through seamless integration with market-leading mass spectrometry, fluorescence and charged aerosol detection. Gain operational simplicity with easy, freely available, one-click workflows via AppsLab and tool-free Viper fittings.


Orbitrap mass spectrometers for intact protein analysis workflows

  Orbitrap Exploris 480 Mass Spectrometer Orbitrap Eclipse Tribrid Mass Spectrometer Q Exactive BioPharma Platform
  Orbitrap Exploris 480 Mass Spectrometer Orbitrap Eclipse Tribrid Mass Spectrometer Q Exactive BioPharma Platform
Advantages Extraordinary simplified within a compact footprint where next level exploration delivers quantifiable accuracy and the selectivity required to obtain richer insights, empowering all by bringing performance and intelligence together to provide rapid confirmation of intact protein molecular weight and determine glycoform heterogeneity of biopharmaceuticals. Go beyond today's discovery with new innovations that deliver the ultimate flexibility to expand experimental scope, with built-in intelligence to ensure the highest data quality and confidence when confirming intact protein molecular weight and determining glycoform heterogeneity of biopharmaceuticals. Realize complete BioPharma characterization with a mass spectrometer which offers distinct operational modes optimized for the top three protein characterization workflows.
Resolving power 480,000 at m/z 195 7,500-500,000 FWHM at m/z 200 (1M option) Up to 240,000 at m/z 200
Scan speed Up to 40Hz Up to 40Hz 18Hz
Mass range 40 to 6,000 m/z (up to 8,000 m/z with the BioPharma option) 50 to 6,000 (up to 8,000 with HMRn) 50 to 8,000 m/z
Dynamic range >5000:1 >5000 with a single OTMS spectrum >5000:1
Mass accuracy Internal <1 ppm RMS; External: <3 ppm RMS Internal <1 ppm RMS; External: <3 ppm RMS Internal <1 ppm RMS; External: <3 ppm RMS


Featured software for intact protein analysis workflows

Powerful, yet simple protein deconvolution and peptide mapping software

Screen, identify, and characterize intact proteins with higher productivity and confidence using the Intact Protein workflow in BioPharma Finder software. Two deconvolution algorithms take full advantage of the high-quality HRAM data produced by Orbitrap mass spectrometers. In addition to intact characterization, you can confirm amino acid sequence, identify site and type of known/unknown post translational modifications, while providing relative amounts using Peptide Mapping workflows. Additional features include disulfide linkage, sequence variant analysis, and sequence alterations.


Featured video

Prof. Alexander Makarov takes us through the evolution of Obitrap technology for the analysis of biotherapeutics.

Featured intact protein analysis resource
Intact and Native Characterization webinars

Join experts from Genentech and Thermo Fisher to get great structural insight into your drug product using the Q Exactive BioPharma platform of mass spectrometers.