Deliver unequivocal intact mass assignments
Among the analytical tools used for the characterization of proteins, high-resolution accurate-mass (HRAM) mass spectrometry has become increasingly important for quantification of the various glycoforms and protein variant analysis. New methods coupling UHPLC with Orbitrap-based mass spectrometers give rapid insight into the proteoform profile for direct comparison of biotherapeutic products during discovery, development, and QC.
Intact protein mass spectrometry workflow
Featured chromatography solutions for intact mass analysis
New Vanquish Flex UHPLC delivers confidence, with a fully bio-compatible flow path and proven compliance. Achieve unprecedented performance in retention time stability, sensitivity and separation efficiency with the widest range of column chemistries for biotherapeutic protein characterization. Demand versatility through seamless integration with market-leading mass spectrometry, fluorescence and charged aerosol detection. Gain operational simplicity with easy, freely available, one-click workflows via AppsLab and tool-free Viper fittings.
Perform high-performance, reversed-phase (RP) LC and LC-MS characterization of monoclonal antibodies (mAbs), fragments, variants, antibody drug conjugates (ADCs), PEGylated proteins, and biospecific proteins using the new MAbPac RP columns. These columns have excellent resolution, long column lifetime, and low carry over. The surface chemistry, particle size, and pore size, have been optimized to for high-resolution separations.
Reduce background contamination and noise seen in LC-MS analysis of samples containing non-volatile salts with a MSPac DS-10 desalting cartridge. Removal of these salts prior to analysis enhances analyte response and improves sensitivity aiding a more reliable identification. Use alone or in combination with an analytical column for high resolution separations.
Orbitrap mass spectrometers for intact mass analysis
|Orbitrap Exploris 480 Mass Spectrometer||Orbitrap Eclipse Tribrid Mass Spectrometer||Q Exactive BioPharma Platform||Exactive Plus EMR Mass Spectrometer|
|Advantages||Extraordinary simplified within a compact footprint where next level exploration delivers quantifiable accuracy and the selectivity required to obtain richer insights, empowering all by bringing performance and intelligence together to provide rapid confirmation of intact protein molecular weight and determine glycoform heterogeneity of biopharmaceuticals.||Go beyond today's discovery with new innovations that deliver the ultimate flexibility to expand experimental scope, with built-in intelligence to ensure the highest data quality and confidence when confirming intact protein molecular weight and determining glycoform heterogeneity of biopharmaceuticals.||Realize complete BioPharma characterization with a mass spectrometer which offers distinct operational modes optimized for the top three protein characterization workflows.||Understand protein assemblies and protein-protein interactions with the optional extended mass range (EMR), and with detection of high-mass ions, it's an outstanding platform for native intact biopharmaceutical applications.|
|Resolving power||480,000 at m/z 195||7,500-500,000 FWHM at m/z 200 (1M option)||Up to 240,000 at m/z 200||Up to 140,000 FWHM|
|Scan speed||Up to 40Hz||Up to 40Hz||18Hz||18Hz|
|Mass range||40 to 6,000 m/z (up to 8,000 m/z with the BioPharma option)||50 to 6,000 (up to 8,000 with HMRn)||50 to 8,000 m/z||350 to 20,000 m/z|
|Dynamic range||>5000:1||>5000 with a single OTMS spectrum||>5000:1||>5000:1|
|Mass accuracy||Internal <1 ppm RMS; External: <3 ppm RMS||Internal <1 ppm RMS; External: <3 ppm RMS||Internal <1 ppm RMS; External: <3 ppm RMS||<5 ppm for m/z 6001 to 10,000|
High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.
Featured software for intact mass analysis
Screen, identify, and characterize intact proteins with higher productivity and confidence using the Intact Protein workflow in BioPharma Finder software. Two deconvolution algorithms take full advantage of the high-quality HRAM data produced by Orbitrap mass spectrometers. In addition to intact characterization, you can confirm amino acid sequence, identify site and type of known/unknown post translational modifications, while providing relative amounts using Peptide Mapping workflows. Additional features include disulfide linkage, sequence variant analysis, and sequence alterations.
Join experts from Genentech and Thermo Fisher to get great structural insight into your drug product using the Q Exactive BioPharma platform of mass spectrometers.