Intact Mass Analysis

Deliver unequivocal intact mass assignments

Among the analytical tools used for the characterization of proteins, high-resolution accurate-mass (HRAM) mass spectrometry has become increasingly important for quantification of the various glycoforms and protein variant analysis. New methods coupling UHPLC with Orbitrap-based mass spectrometers give rapid insight into the proteoform profile for direct comparison of biotherapeutic products during discovery, development, and QC.

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Intact protein mass spectrometry workflow

Intact mass analysis workflow

NEW intact protein chromatography built for biopharma

New Vanquish Flex UHPLC delivers confidence, with a fully bio-compatible flow path and proven compliance. Achieve unprecedented performance in retention time stability, sensitivity and separation efficiency with the widest range of column chemistries for biotherapeutic protein characterization. Demand versatility through seamless integration with market-leading mass spectrometry, fluorescence and charged aerosol detection. Gain operational simplicity with easy, freely available, one-click workflows via AppsLab and tool-free Viper fittings.

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Orbitrap mass spectrometers for intact mass analysis

  Orbitrap Exploris 480 Mass Spectrometer Orbitrap Eclipse Tribrid Mass Spectrometer Q Exactive BioPharma Platform Exactive Plus EMR Mass Spectrometer
  Orbitrap Exploris 480 Mass Spectrometer Orbitrap Eclipse Tribrid Mass Spectrometer Q Exactive BioPharma Platform Q Exactive BioPharma Platform
Advantages Extraordinary simplified within a compact footprint where next level exploration delivers quantifiable accuracy and the selectivity required to obtain richer insights, empowering all by bringing performance and intelligence together to provide rapid confirmation of intact protein molecular weight and determine glycoform heterogeneity of biopharmaceuticals. Go beyond today's discovery with new innovations that deliver the ultimate flexibility to expand experimental scope, with built-in intelligence to ensure the highest data quality and confidence when confirming intact protein molecular weight and determining glycoform heterogeneity of biopharmaceuticals. Realize complete BioPharma characterization with a mass spectrometer which offers distinct operational modes optimized for the top three protein characterization workflows. Understand protein assemblies and protein-protein interactions with the optional extended mass range (EMR), and with detection of high-mass ions, it's an outstanding platform for native intact biopharmaceutical applications.
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Resolving power 480,000 at m/z 195 7,500-500,000 FWHM at m/z 200 (1M option) Up to 240,000 at m/z 200 Up to 140,000 FWHM
Scan speed Up to 40Hz Up to 40Hz 18Hz 18Hz
Mass range 40 to 6,000 m/z (up to 8,000 m/z with the BioPharma option) 50 to 6,000 (up to 8,000 with HMRn) 50 to 8,000 m/z 350 to 20,000 m/z
Dynamic range >5000:1 >5000 with a single OTMS spectrum >5000:1 >5000:1
Mass accuracy Internal <1 ppm RMS; External: <3 ppm RMS Internal <1 ppm RMS; External: <3 ppm RMS Internal <1 ppm RMS; External: <3 ppm RMS <5 ppm for m/z 6001 to 10,000

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Boost selectivity and enhance your proteomics workflow with FAIMS Pro Interface

High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.

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Featured software for intact mass analysis

Powerful, yet simple protein deconvolution and peptide mapping software

Screen, identify, and characterize intact proteins with higher productivity and confidence using the Intact Protein workflow in BioPharma Finder software. Two deconvolution algorithms take full advantage of the high-quality HRAM data produced by Orbitrap mass spectrometers. In addition to intact characterization, you can confirm amino acid sequence, identify site and type of known/unknown post translational modifications, while providing relative amounts using Peptide Mapping workflows. Additional features include disulfide linkage, sequence variant analysis, and sequence alterations.

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Featured videos


Featured intact protein analysis resource
Intact and Native Characterization webinars
Intact and Native Characterization webinars

Join experts from Genentech and Thermo Fisher to get great structural insight into your drug product using the Q Exactive BioPharma platform of mass spectrometers.