Deliver unequivocal intact mass assignments
Among the analytical tools used for the characterization of proteins, high-resolution accurate-mass (HRAM) mass spectrometry has become increasingly important for quantification of the various glycoforms and protein variant analysis. New methods coupling UHPLC with Orbitrap-based mass spectrometers give rapid insight into the proteoform profile for direct comparison of biotherapeutic products during discovery, development, and QC.
Intact protein mass spectrometry workflow
Intact mass analysis categories
Perform high-performance, reversed-phase (RP) LC and LC-MS characterization of monoclonal antibodies (mAbs), fragments, variants, antibody drug conjugates (ADCs), PEGylated proteins, and biospecific proteins using the new MAbPac RP columns. These columns have excellent resolution, long column lifetime, and low carry over. The surface chemistry, particle size, and pore size, have been optimized to for high-resolution separations.
The new Q Exactive Biopharma Platform of mass spectrometers now add superior denatured and native MS intact analysis and subunit top/middle-down analysis capabilities to the most powerful benchtop peptide mapping instruments available. High Mass Range (HMR) mode enables increased scanning range up to 8,000 m/z, providing the flexibility to analyze your intact proteins in either denaturing or native MS conditions. Now the gold standard in peptide mapping excels in intact mass analysis too.
Screen, identify, and characterize intact proteins with higher productivity and confidence using the Intact Protein workflow in BioPharma Finder software. Two deconvolution algorithms take full advantage of the high-quality HRAM data produced by Orbitrap mass spectrometers. In addition to intact characterization, you can confirm amino acid sequence, identify site and type of known/unknown post translational modifications, while providing relative amounts using Peptide Mapping workflows. Additional features include disulfide linkage, sequence variant analysis, and sequence alterations.
New Vanquish Flex UHPLC delivers confidence, with a fully bio-compatible flow path and proven compliance. Achieve unprecedented performance in retention time stability, sensitivity and separation efficiency with the widest range of column chemistries for biotherapeutic protein characterization. Demand versatility through seamless integration with market-leading mass spectrometry, fluorescence and charged aerosol detection. Gain operational simplicity with easy, freely available, one-click workflows via AppsLab and tool-free Viper fittings.
With the optional extended mass range (EMR) of up to m/z 20,000 and improved detection of high-mass ions, the Exactive Plus EMR mass spectrometer is an outstanding platform for biopharmaceutical applications requiring analysis of monoclonal antibodies (mAbs), antibody drug conjugates (ADCs), protein assemblies, and other proteins in their intact and native states.
Join experts from Genentech and Thermo Fisher to get great structural insight into your drug product using the Q Exactive BioPharma platform of mass spectrometers.