Primary hepatocytes isolated from the liver are effective tools for the in vitro evaluation of metabolism, drug-drug interactions, hepatotoxicity, and transporter activity.
Gibco hepatocytes offer high viabilities routinely great than 80%, in vivo-like enzyme expression levels, and are characterized for phase I and phase II drug metabolizing enzyme activities. An extensive selection of lots and multiple large lots are available – ideal for reproducible long-term and multi-site studies.
Gibco Human Spheroid-Qualified Hepatocytes
Our Spheroid qualified hepatocytes are the first off-the-shelf cryopreserved human cells for generation of hepatic spheroids in your own lab. Each vial contains a minimum of five million viable cells, sufficient for up to sixteen 96-well plates of hepatic spheroids.
- Maintain cells in culture longer—up to 21 days, >4X longer than cells last in monolayer culture
- Design your own schedule—cells are ready for plating when you are
- Interrogate pathways of interest—applicable for toxicity, metabolism, or disease modeling
Gibco Hepatic Cells and related products
Gibco human hepatic cells
Gibco human hepatocytes are isolated in-house by our experienced hepatic cell team. Our dedicated technical team are committed to meeting all your ADME needs.
- Extensive expertise with decades of hepatocyte culture experience
- A broad selection of cells, media, and technical resources necessary to create physiologically relevant co-culture models
- Broad selection of human and animal hepatocytes
Plateable
cells
cells
- Transporter-qualified—Ideally suited for influx studies requiring >5 days culture
- Induction-qualified—hepatocytes ideally suited for use in in vitro enzyme induction studies
- Metabolism-qualified—suitable for plated metabolism applications continuing >3 days
- Spheroid-qualified—Hepatocytes pre-qualified to for spheroid models
- Pooled hepatocytes—5-donor plateable pool to help minimize lot-to-lot variability
Suspension
cells
cells
- Transporter-qualified—short term uptake and efflux studies
- Metabolism-qualified—suitable for short-term suspension metabolism applications
- Polymorphic donor—Hepatocytes that have been pharmacogenetically characterized to contain homozygous null alleles (e.g. CYP2D6*4⁄*4) via genotyping analysis
- Suspension pooled—10-donor or 50-donor pooled population of suspension hepatocytes
Non-parenchymal cells
Cell
lines
lines
- HepaRG—An immortalized hepatic cell line that retains many characteristics of primary human hepatocytes
Animal hepatic cells
Gibco animal hepatocytes are isolated in-house by our experienced hepatic cell team. All our high-viability animal hepatocyte cells are characterized for Phase I and Phase II drug metabolizing enzymes. We offer multiple species to meet all your ADME needs including mouse, rat, dog, and monkey.
- Extensive expertise with years of hepatocyte culture experience
- Dedicated technical and field support to help with your ADME/DMPK research
- Broad selection of human and animal hepatic cells to support your ADME/DMPK research
Plateable cells
Suspension cells
Media, supplements, and matrices
Media & supplements
Ensure a successful setup with the right tools. Whether you’re just starting and need a recovery medium specially designed to improve the viability and overall quality of cryopreserved hepatocytes, need an enriched wash medium to reduce membrane leakage and ease of centrifugation, are ready to setup your culture with hepatocyte plating supplements and William’s E Medium , or supplementing cells for long-term cell culture feeding, we offer premier Gibco media for all hepatocyte cell culture stages.
Our breadth of Gibco media and reagents provide an ideal environment for adherent and suspension hepatocytes.
- Supplements and media for adherent and suspension culture types
- Media for every step in your cell culture workflow
- Ease of transfer—we culture our hepatocytes with our own products
- Customizable medium—begin your custom journey here
Hepatocyte media & supplements
- Hepatocyte Thaw Medium
- Hepatocyte Recovery Medium
- Hepatocyte Wash Medium
- Primary Hepatocyte Thawing and Plating Supplements
- William’s E Medium
- Primary Hepatocyte Maintenance Supplements
- HepExtend Supplement
Extracellular matrices
Extracellular matrices offer more in vivo like morphology and physiologically relevant environments for improved hepatocyte cell biology function. We offer numerous extracellular matrices (ECMs) for culturing hepatocytes in 2D and 3D, from Geltrex, pre-plated collagen in 6-, 24-, or 96-well formats, to rat or bovine collagen I protein, and AlgiMatrix reagent a user friendly, animal-free bioscaffold for the creating higher-fidelity cell models that are more predictive of disease states and drug responses.
Gibco human hepatic cells
Gibco human hepatocytes are isolated in-house by our experienced hepatic cell team. Our dedicated technical team are committed to meeting all your ADME needs.
- Extensive expertise with decades of hepatocyte culture experience
- A broad selection of cells, media, and technical resources necessary to create physiologically relevant co-culture models
- Broad selection of human and animal hepatocytes
Plateable
cells
cells
- Transporter-qualified—Ideally suited for influx studies requiring >5 days culture
- Induction-qualified—hepatocytes ideally suited for use in in vitro enzyme induction studies
- Metabolism-qualified—suitable for plated metabolism applications continuing >3 days
- Spheroid-qualified—Hepatocytes pre-qualified to for spheroid models
- Pooled hepatocytes—5-donor plateable pool to help minimize lot-to-lot variability
Suspension
cells
cells
- Transporter-qualified—short term uptake and efflux studies
- Metabolism-qualified—suitable for short-term suspension metabolism applications
- Polymorphic donor—Hepatocytes that have been pharmacogenetically characterized to contain homozygous null alleles (e.g. CYP2D6*4⁄*4) via genotyping analysis
- Suspension pooled—10-donor or 50-donor pooled population of suspension hepatocytes
Non-parenchymal cells
Cell
lines
lines
- HepaRG—An immortalized hepatic cell line that retains many characteristics of primary human hepatocytes
Animal hepatic cells
Gibco animal hepatocytes are isolated in-house by our experienced hepatic cell team. All our high-viability animal hepatocyte cells are characterized for Phase I and Phase II drug metabolizing enzymes. We offer multiple species to meet all your ADME needs including mouse, rat, dog, and monkey.
- Extensive expertise with years of hepatocyte culture experience
- Dedicated technical and field support to help with your ADME/DMPK research
- Broad selection of human and animal hepatic cells to support your ADME/DMPK research
Plateable cells
Suspension cells
Media, supplements, and matrices
Media & supplements
Ensure a successful setup with the right tools. Whether you’re just starting and need a recovery medium specially designed to improve the viability and overall quality of cryopreserved hepatocytes, need an enriched wash medium to reduce membrane leakage and ease of centrifugation, are ready to setup your culture with hepatocyte plating supplements and William’s E Medium , or supplementing cells for long-term cell culture feeding, we offer premier Gibco media for all hepatocyte cell culture stages.
Our breadth of Gibco media and reagents provide an ideal environment for adherent and suspension hepatocytes.
- Supplements and media for adherent and suspension culture types
- Media for every step in your cell culture workflow
- Ease of transfer—we culture our hepatocytes with our own products
- Customizable medium—begin your custom journey here
Hepatocyte media & supplements
- Hepatocyte Thaw Medium
- Hepatocyte Recovery Medium
- Hepatocyte Wash Medium
- Primary Hepatocyte Thawing and Plating Supplements
- William’s E Medium
- Primary Hepatocyte Maintenance Supplements
- HepExtend Supplement
Extracellular matrices
Extracellular matrices offer more in vivo like morphology and physiologically relevant environments for improved hepatocyte cell biology function. We offer numerous extracellular matrices (ECMs) for culturing hepatocytes in 2D and 3D, from Geltrex, pre-plated collagen in 6-, 24-, or 96-well formats, to rat or bovine collagen I protein, and AlgiMatrix reagent a user friendly, animal-free bioscaffold for the creating higher-fidelity cell models that are more predictive of disease states and drug responses.
With the prevalence of lifestyle-influenced liver diseases, such as non-alcoholic steatohepatitis (NASH), which can advance to fibrosis (scarring of the tissue) leading to liver cirrhosis or liver disease, there is an urgent need for the development of novel drug treatments. Find out how our scientists are working to create more physiologically relevant models, using new techniques for 3D and new approaches to co-cultures. Learn more about some of more recent presentations and presented posters below and a full listing in our ADME/Tox resource center.
- Co-culture of cryopreserved primary Kupffer cells and hepatocytes to study APAP cytotoxicity
- Co-culture of hepatocytes and Kupffer cells as a model for liver inflammation
- Cytokine-induced liver hepatotoxicity of trovafloxacin in co-culture of hepatocytes and Kupffer cells
- Co-culture of primary hepatic stellate cells in modeling of liver in 2D and 3D
Resources
Support
Drug Discovery & Development Support Center
Find tips, troubleshooting help, and resources for support related to your drug discovery experiments.
For Research Use Only. Not for use in diagnostic procedures.
