Hepatocytes

Primary hepatocytes isolated from the liver are effective tools for the in vitro evaluation of metabolism, drug-drug interactions, hepatotoxicity, and transporter activity. 

Gibco hepatocytes offer high viabilities routinely great than 80%, in vivo-like enzyme expression levels, and are characterized for phase I and phase II drug metabolizing enzyme activities. An extensive selection of lots and multiple large lots are available – ideal for reproducible long-term and multi-site studies.

Request more information

Gibco Human Spheroid-Qualified Hepatocytes

Our Spheroid qualified hepatocytes are the first off-the-shelf cryopreserved human cells for generation of hepatic spheroids in your own lab. Each vial contains a minimum of five million viable cells, sufficient for up to sixteen 96-well plates of hepatic spheroids.

  • Maintain cells in culture longer—up to 21 days, >4X longer than cells last in monolayer culture
  • Design your own schedule—cells are ready for plating when you are
  • Interrogate pathways of interest—applicable for toxicity, metabolism, or disease modeling

Learn how you can research in the third dimension today

Gibco Hepatic Cells and related products

Gibco human hepatic cells

Gibco human hepatocytes are isolated in-house by our experienced hepatic cell team. Our dedicated technical team are committed to meeting all your ADME needs.

  • Extensive expertise with decades of hepatocyte culture experience
  • A broad selection of cells, media, and technical resources necessary to create physiologically relevant co-culture models
  • Broad selection of human and animal hepatocytes

Plateable
cells

microscopic view of plateable hepatocytes

Suspension
cells

Vial of Gibco hepatocytes
  • Transporter-qualified—short term uptake and efflux studies
  • Metabolism-qualified—suitable for short-term suspension metabolism applications
  • Polymorphic donor—Hepatocytes that have been pharmacogenetically characterized to contain homozygous null alleles (e.g. CYP2D6*4⁄*4) via genotyping analysis
  • Suspension pooled—10-donor or 50-donor pooled population of suspension hepatocytes

Cell
lines

microscopic view of a HepaRG spheroid
  • HepaRG—An immortalized hepatic cell line that retains many characteristics of primary human hepatocytes

Animal hepatic cells

Gibco animal hepatocytes are isolated in-house by our experienced hepatic cell team. All our high-viability animal hepatocyte cells are characterized for Phase I and Phase II drug metabolizing enzymes. We offer multiple species to meet all your ADME needs including mouse, rat, dog, and monkey.

  • Extensive expertise with years of hepatocyte culture experience
  • Dedicated technical and field support to help with your ADME/DMPK research
  • Broad selection of human and animal hepatic cells to support your ADME/DMPK research

Plateable cells

  • Suitable for plated metabolism applications

Suspension cells

  • Suitable for short-term metabolism applications

Non-parenchymal cells

  • Cryopreserved kupffers for study of diverse hepatic functions

Media, supplements, and matrices

Media & supplements

Ensure a successful setup with the right tools. Whether you’re just starting and need a recovery medium specially designed to improve the viability and overall quality of cryopreserved hepatocytes, need an enriched wash medium to reduce membrane leakage and ease of centrifugation, are ready to setup your culture with hepatocyte plating supplements and William’s E Medium , or supplementing cells for long-term cell culture feeding, we offer premier Gibco media for all hepatocyte cell culture stages.

Our breadth of Gibco media and reagents provide an ideal environment for adherent and suspension hepatocytes.

  • Supplements and media for adherent and suspension culture types
  • Media for every step in your cell culture workflow
  • Ease of transfer—we culture our hepatocytes with our own products
  • Customizable medium—begin your custom journey here

Hepatocyte media & supplements

Extracellular matrices

Extracellular matrices offer more in vivo like morphology and physiologically relevant environments for improved hepatocyte cell biology function. We offer numerous extracellular matrices (ECMs) for culturing hepatocytes in 2D and 3D, from Geltrex, pre-plated collagen in 6-, 24-, or 96-well formats, to rat or bovine collagen I protein, and AlgiMatrix reagent a user friendly, animal-free bioscaffold for the creating higher-fidelity cell models that are more predictive of disease states and drug responses.

Gibco human hepatic cells

Gibco human hepatocytes are isolated in-house by our experienced hepatic cell team. Our dedicated technical team are committed to meeting all your ADME needs.

  • Extensive expertise with decades of hepatocyte culture experience
  • A broad selection of cells, media, and technical resources necessary to create physiologically relevant co-culture models
  • Broad selection of human and animal hepatocytes

Plateable
cells

microscopic view of plateable hepatocytes

Suspension
cells

Vial of Gibco hepatocytes
  • Transporter-qualified—short term uptake and efflux studies
  • Metabolism-qualified—suitable for short-term suspension metabolism applications
  • Polymorphic donor—Hepatocytes that have been pharmacogenetically characterized to contain homozygous null alleles (e.g. CYP2D6*4⁄*4) via genotyping analysis
  • Suspension pooled—10-donor or 50-donor pooled population of suspension hepatocytes

Cell
lines

microscopic view of a HepaRG spheroid
  • HepaRG—An immortalized hepatic cell line that retains many characteristics of primary human hepatocytes

Animal hepatic cells

Gibco animal hepatocytes are isolated in-house by our experienced hepatic cell team. All our high-viability animal hepatocyte cells are characterized for Phase I and Phase II drug metabolizing enzymes. We offer multiple species to meet all your ADME needs including mouse, rat, dog, and monkey.

  • Extensive expertise with years of hepatocyte culture experience
  • Dedicated technical and field support to help with your ADME/DMPK research
  • Broad selection of human and animal hepatic cells to support your ADME/DMPK research

Plateable cells

  • Suitable for plated metabolism applications

Suspension cells

  • Suitable for short-term metabolism applications

Non-parenchymal cells

  • Cryopreserved kupffers for study of diverse hepatic functions

Media, supplements, and matrices

Media & supplements

Ensure a successful setup with the right tools. Whether you’re just starting and need a recovery medium specially designed to improve the viability and overall quality of cryopreserved hepatocytes, need an enriched wash medium to reduce membrane leakage and ease of centrifugation, are ready to setup your culture with hepatocyte plating supplements and William’s E Medium , or supplementing cells for long-term cell culture feeding, we offer premier Gibco media for all hepatocyte cell culture stages.

Our breadth of Gibco media and reagents provide an ideal environment for adherent and suspension hepatocytes.

  • Supplements and media for adherent and suspension culture types
  • Media for every step in your cell culture workflow
  • Ease of transfer—we culture our hepatocytes with our own products
  • Customizable medium—begin your custom journey here

Hepatocyte media & supplements

Extracellular matrices

Extracellular matrices offer more in vivo like morphology and physiologically relevant environments for improved hepatocyte cell biology function. We offer numerous extracellular matrices (ECMs) for culturing hepatocytes in 2D and 3D, from Geltrex, pre-plated collagen in 6-, 24-, or 96-well formats, to rat or bovine collagen I protein, and AlgiMatrix reagent a user friendly, animal-free bioscaffold for the creating higher-fidelity cell models that are more predictive of disease states and drug responses.

With the prevalence of lifestyle-influenced liver diseases, such as non-alcoholic steatohepatitis (NASH), which can advance to fibrosis (scarring of the tissue) leading to liver cirrhosis or liver disease, there is an urgent need for the development of novel drug treatments. Find out how our scientists are working to create more physiologically relevant models, using new techniques for 3D and new approaches to co-cultures. Learn more about some of more recent presentations and presented posters below and a full listing in our ADME/Tox resource center.

Resources

Video: Thawing and Plating Cryopreserved Hepatocytes ›

Support

Drug Discovery & Development Support Center

Find tips, troubleshooting help, and resources for support related to your drug discovery experiments.