Many of the PerkinElmer plate readers including the Envision®, ViewLux® and VICTOR™ are compatible with Life Technologies assay technologies for TR-FRET, FRET, Fluorescence Intensity (kinetic), and Fluorescence Polarization.

Before using LanthaScreen® Terbium and Europium assays

Test your plate reader set-up

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen® assay.

Download Terbium assay application note

Download Europium assay application note

EnVision® Multilabel Reader

 
 

 

Instrument Configuration:

Assay Technology Adapta® &
LanthaScreen®
Europium
GeneBLAzer®LanthaScreen®
Terbium
Predictor™Z'-LYTE®
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup Guide   

Application Note-----
Poster--Lab Auto 08--
Excitation Filter (15 mm)340/30
(PV00215)
400/25340/30
(PV00215)
531/25 FP400/25
Emission 1 Filter (15 mm)615/8.5460/25495/10
(PV00315)
579/25 FP P-pol460/25
Emission 2 Filter (15 mm)665/7.5535/25520/25
(PV00315)
579 FP S-pol535/25
Dichroic MirrorD400/630 TRF Dual or D400 TRFD425/495 BLA Dual or D425 BLA; 50/50 General Dual or 50/50 GeneralD400/630 TRF Dual or D400 TRF; D400/505 LanthaScreen® DualD555fp/D595 Dual or D555fpD425/495 BLA Dual or D425 BLA; 50/50 General Dual or 50/50 General

 

VICTOR™ Multilabel Plate Readers

Instrument Configuration:

Assay Technology Adapta® &
LanthaScreen®
Europium
GeneBLAzer®LanthaScreen®
Terbium
Predictor™Z'-LYTE®
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup GuideReader compatible but settings not validatedReader not
compatible
 Reader not
compatible
Reader not
tested
Application Note-----
Poster-----
Excitation Filter (15 mm)340/60
(built-in)
-340/60
(built-in)
-NA
Emission 1 Filter (25 mm)616/8.5-495/10
(PV00325)
-NA
Emission 2 Filter (25 mm)665/7.5-520/25
(PV00325)
-NA

 


 

Instrument Configuration:

Assay Technology Adapta® &
LanthaScreen®
Europium
GeneBLAzer®LanthaScreen®
Terbium
Predictor™Z'-LYTE®
ModalityTR-FRETFRET (bottom)TR-FRETN/AN/A
Setup GuideReader compatible but settings not validatedReader not
tested
 Reader not
tested
Reader not
tested
Application Note-----
Poster--Lab Auto 08--
Excitation Filter (60 mm)340/60NA340/60NANA
Emission 1 Filter (60 mm)616/8NA495/10
(Chroma)
NANA
Emission 2 Filter (60 mm)671/7.8NA520/20NANA
Dichroic Mirror400NA400NANA



* Filters (diameter) shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact PerkinElmer for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above

** Some compatible filter sets are available for purchase from Life Technologies.  Click on selected filter sets for more information.

Envision® and ViewLux® are registered trademarks and Victor™ is a trademark of PerkinElmer.

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