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Tecan's Infinite® M1000/M1000 PRO and Infinite® F500 have received the LanthaScreen® Certified Plus designation and Tecan’s Infinite® F200/200 PRO have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance.

Before using LanthaScreen® Terbium and Europium assays

Test your plate reader set-up

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen® assay.

Download Terbium assay application note

Download Europium assay application note

Infinite® M1000/M1000 PRO

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

Application Note - - - View -
Poster - - - - -
Excitation 317/20 409/12 332/20 530/5 405/12
Emission 1 620/12 460/12 490/10 585/20 445/12 
Emission 2 665/12 530/12 520/20 585/20  520/12 

 

Infinite® F500

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

Application Note - -   View -
Poster - - - - -
Excitation Filter (12.5 mm) 340/30
(PV002125)
415/20 340/30
(PV002125)
535/25 415/20
Emission 1 Filter (12.5 mm) 615/10 460/20 495/10
(PV003125)
590/20 460/20 
Emission 2 Filter (12.5 mm) 665/10 535/25 520/25
(PV003125)
590/20  535/25
Dichroic Mirror 510 NA 50% or 510 560 50%

 

Infinite® F200/200 PRO

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

Reader not tested

Application Note - - - - -
Poster - - - - -
Excitation Filter (12.5 mm) 340/30
(PV002125)
409/20 340/30
(PV002125)
NA 415/20
Emission 1 Filter (12.5 mm) 615/10 460/20 495/10
(PV003125)
NA 460/20 
Emission 2 Filter (12.5 mm) 665/10 525/25 520/25
(PV003125)
NA 535/25
Dichroic Mirror 510 NA 50% or 510 NA 50%

 

Safire2

 

Instrument Configuration:

Assay Technology  Adapta® &
LanthaScreen®
Europium
GeneBLAzer® LanthaScreen®
Terbium
Predictor™ Z'-LYTE®
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

Application Note - - - - -
Poster - - View - -
Excitation 317/20  409/12 332/20 530/20 405/12 
Emission 1 620/12 460/12 485/20 585/20 445/12 
Emission 2 665/12 530/12 515/20 585/20 520/12 



* Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Tecan for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above

** Some compatible filter sets are available for purchase from Life Technologies.  Click on selected filter sets for more information

Infinite® is a registered trademark and Safire2™ is a trademark of Tecan Group Ltd.