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Tecan's Infinite® M1000/M1000 PRO and Infinite® F500 have received the LanthaScreen® Certified Plus designation and Tecan’s Infinite® F200/200 PRO have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance.

Before using LanthaScreen® Terbium and Europium assays

Test your plate reader set-up

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen® assay.

Download Terbium assay application note

Download Europium assay application note

Infinite® M1000/M1000 PRO

Instrument Configuration:

Assay Technology Adapta® &
LanthaScreen®
Europium
GeneBLAzer®LanthaScreen®
Terbium
Predictor™Z'-LYTE®
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup Guide

Application Note---View-
Poster-----
Excitation317/20409/12332/20530/5405/12
Emission 1620/12460/12490/10585/20445/12 
Emission 2665/12530/12520/20585/20 520/12 

 

Infinite® F500

Instrument Configuration:

Assay Technology Adapta® &
LanthaScreen®
Europium
GeneBLAzer®LanthaScreen®
Terbium
Predictor™Z'-LYTE®
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup Guide

Application Note-- View-
Poster-----
Excitation Filter (12.5 mm)340/30
(PV002125)
415/20340/30
(PV002125)
535/25415/20
Emission 1 Filter (12.5 mm)615/10460/20495/10
(PV003125)
590/20460/20 
Emission 2 Filter (12.5 mm)665/10535/25520/25
(PV003125)
590/20 535/25
Dichroic Mirror510NA50% or 51056050%

 

Infinite® F200/200 PRO

Instrument Configuration:

Assay Technology Adapta® &
LanthaScreen®
Europium
GeneBLAzer®LanthaScreen®
Terbium
Predictor™Z'-LYTE®
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup Guide

Reader not tested

Application Note-----
Poster-----
Excitation Filter (12.5 mm)340/30
(PV002125)
409/20340/30
(PV002125)
NA415/20
Emission 1 Filter (12.5 mm)615/10460/20495/10
(PV003125)
NA460/20 
Emission 2 Filter (12.5 mm)665/10525/25520/25
(PV003125)
NA535/25
Dichroic Mirror510NA50% or 510NA50%

 

Safire2

 

Instrument Configuration:

Assay Technology Adapta® &
LanthaScreen®
Europium
GeneBLAzer®LanthaScreen®
Terbium
Predictor™Z'-LYTE®
ModalityTR-FRETFRET (bottom)TR-FRETFPFRET
Setup Guide

Application Note-----
Poster--View--
Excitation317/20 409/12332/20530/20405/12 
Emission 1620/12460/12485/20585/20445/12 
Emission 2665/12530/12515/20585/20520/12 



* Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Tecan for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above

** Some compatible filter sets are available for purchase from Life Technologies.  Click on selected filter sets for more information

Infinite® is a registered trademark and Safire2™ is a trademark of Tecan Group Ltd.

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