Drug Discovery HTS Assay Types & Definitions
Life Technologies offers a wide range of high-throughput screening assays for target and lead discovery research. Use the table below to view our available assay types; and find the components necessary to make one of these assays with our Drug Discovery Assay Maker™ tool.
Can’t find the assay you need? Our dedicated Custom Biology Services team can work with you and create a custom assay meeting your defined requirements.
If you have any questions about any of these assay technologies, please contact our Drug Discovery Technical Support team.
|Biochemical ADP accumulation||Adapta®||TR-FRET assay for the detection of ADP produced by kinases or other ATP dependent enzymes|
|Biochemical binding||Predictor™ hERG||A set of validated components to perform hERG channel biochemical binding studies in the absence of radioligand|
|Biochemical binding/kinetic||LanthaScreen® Kinase Binding Assay||A simple mix and read TR-FRET assay where binding or displacement of an ATP competitive Alexa Fluor® conjugate or “tracer" to a kinase|
|Biochemical competitive binding||PolarScreen®||Nuclear receptor ligand domain, binding assay that includes protein, a proprietary fluorescent Fluormone™ ligand, and an optimized buffer system|
|Biochemical coregulator interaction||LanthaScreen® NR Co-regulator Interaction Assay||TR-FRET based assay to evaluate recruitment or diplacement of a fluorescein-labeled coregulator peptide. Evaluated conformational changes of nuclear receptors upon ligand binding distinguishing agonists from antagonists.|
|Biochemcial europium phospho-specific activity||LanthaScreen® Activity Assay||TR-FRET, Europium based, kinase activity assay|
|Biochemical phospho-specific activity (no antibody)||Z’-LYTE®||FRET based activity assayfor selectivity profiling. It is the primary assay used in our SelectScreen® kinase profiling service.|
|Biochemical terbium phospho-specific activity||LanthaScreen® Activity Assay||TR-FRET, Terbium based ,kinase activity|
|BacMam virus for cellular expression||BacMam||A modified insect cell virus (baculovirus) that acts as a vehicle to efficiently deliver and express genes in mammalian cells|
|Cell-based beta lactamase reporter gene||CellSensor®
|Measures compound potency and selectivity and allow for analysis, in a high-throughput manner, of key pathways activated or down-regulated by compounds.|
|Cell-based beta-arrestin recruitment||Tango®||Platform based upon ligand binding to a specific GPCR that triggers desensitization; ensuring a selective readout|
|Cell-based calcium flux||Fluo-4||Calcium assay formulated to achieve largest assay window by suppressing background fluorescence generated from media with little-to-no impact on the cellular fluorescence|
|Cell-based potassium flux||FluxOR®||An optically based, homogenous assay for high throughput screening (HTS) measurements of potassium ion channel and transporter activities|
|Cell-based terbium modification activity (transient)||LanthaScreen® BacMam-enabled cellular assay||An efficient and robust method for interrogating specific signal transduction events in a cell background of choice|
|Cell-based terbium phospho-specific activity (stable)||LanthaScreen® cellular assay||TR-FRET-based assays to monitor protein modification within the native cell signaling environment|