Let us help you evaluate your compound’s transporter interactions. With a comprehensive offering of transporter-qualified hepatocytes and vesicles, we can help you in the prediction of bioavailability and clearance of your compound, as well as the potential for drug-drug interactions and potential toxicities.
GenoMembrane TRANSiPORT human SLC transporter cells
GenoMembrane TRANSiPORT human SLC transporter cells are HEK293 cells that transiently overexpress a solute carrier (SLC) transporter protein. Solute carriers are one of two superfamilies of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes into and out of cells. Membrane transporters are found in hepatic cells, kidney proximal tubule cells, intestinal cells, and brain cells.
- Assess potential for transporter-mediated drug metabolism
- Easy-to-use format
- Obtain high-quality results with a large signal-to-noise ratio
GenoMembrane ATP-Binding Cassette (ABC) Transporter Vesicles
GenoMembrane ABC Transporter Vesicles
- Provides easy, targeted assessment of efflux transporters
- Have high signal-to-back ratios
- Are available for a wide selection or species and transporters
ATP-Binding Cassette (ABC) Transporter Vesicles
ATP-binding cassette (ABC) transporter vesicles are easy-to-use, efficient reagents for early assessment of a drug candidate’s substrate and drug interaction potential. Prepared from Sf9 cells which have been engineered to over-express specific ABC transporters, these “inside-out” vesicles provide high levels of transporter activity with low background, giving you a clear signal if your compound is a substrate or inhibitor of a specific efflux transporter. ABC Transporter Vesicles, which have an inside-out structure, are prepared from ABC transporter membranes for use in vesicular transport assays. While ABC transporters typically mediate the efflux of substrates from cells, transporters expressed on these inside-out vesicles import substrates into the vesicles. It is therefore possible to quantitatively evaluate transport activity for your compound by determining the amount incorporated into the vesicles.
GenoMembrane’s products are prepared by technically advanced methodologies using plasma membrane purified from an insect cell system (Sf9 cells transfected with baculovirus) over-expressing ABC transporters. Our vesicles have superior activities compared to competitor products.
Note: One vial of vesicle product can perform 50 vesicular transport assays. We are licensed to offer GenoMembrane products globally except in Japan.
Transporter-Qualified Hepatocytes
Primary hepatocytes are effective tools for the in vitro evaluation of metabolism, drug-drug interactions, hepatotoxicity, and transporter assessment. At Thermo Fisher Scientific, our hepatocyte isolationists are extensively trained in proper techniques to ensure optimal cell health. As a result, Gibco hepatocytes have high viabilities, in vivo-like enzyme expression levels, and if released as plateable cells, excellent confluencies that contribute to polarization and functioning cell-cell contacts.
- Contain functional membrane receptors and transporters
- Facilitate effective transporter uptake and basolateral efflux (plated) experiments
- Have stringent release specs: ≥80% viability and ≥80% confluency (plated cells)
GenoMembrane TRANSiPORT human SLC transporter cells
GenoMembrane TRANSiPORT human SLC transporter cells are HEK293 cells that transiently overexpress a solute carrier (SLC) transporter protein. Solute carriers are one of two superfamilies of membrane transporters that can affect pharmacokinetics and drug exposure by governing the transport of solutes into and out of cells. Membrane transporters are found in hepatic cells, kidney proximal tubule cells, intestinal cells, and brain cells.
- Assess potential for transporter-mediated drug metabolism
- Easy-to-use format
- Obtain high-quality results with a large signal-to-noise ratio
GenoMembrane ATP-Binding Cassette (ABC) Transporter Vesicles
GenoMembrane ABC Transporter Vesicles
- Provides easy, targeted assessment of efflux transporters
- Have high signal-to-back ratios
- Are available for a wide selection or species and transporters
ATP-Binding Cassette (ABC) Transporter Vesicles
ATP-binding cassette (ABC) transporter vesicles are easy-to-use, efficient reagents for early assessment of a drug candidate’s substrate and drug interaction potential. Prepared from Sf9 cells which have been engineered to over-express specific ABC transporters, these “inside-out” vesicles provide high levels of transporter activity with low background, giving you a clear signal if your compound is a substrate or inhibitor of a specific efflux transporter. ABC Transporter Vesicles, which have an inside-out structure, are prepared from ABC transporter membranes for use in vesicular transport assays. While ABC transporters typically mediate the efflux of substrates from cells, transporters expressed on these inside-out vesicles import substrates into the vesicles. It is therefore possible to quantitatively evaluate transport activity for your compound by determining the amount incorporated into the vesicles.
GenoMembrane’s products are prepared by technically advanced methodologies using plasma membrane purified from an insect cell system (Sf9 cells transfected with baculovirus) over-expressing ABC transporters. Our vesicles have superior activities compared to competitor products.
Note: One vial of vesicle product can perform 50 vesicular transport assays. We are licensed to offer GenoMembrane products globally except in Japan.
Transporter-Qualified Hepatocytes
Primary hepatocytes are effective tools for the in vitro evaluation of metabolism, drug-drug interactions, hepatotoxicity, and transporter assessment. At Thermo Fisher Scientific, our hepatocyte isolationists are extensively trained in proper techniques to ensure optimal cell health. As a result, Gibco hepatocytes have high viabilities, in vivo-like enzyme expression levels, and if released as plateable cells, excellent confluencies that contribute to polarization and functioning cell-cell contacts.
- Contain functional membrane receptors and transporters
- Facilitate effective transporter uptake and basolateral efflux (plated) experiments
- Have stringent release specs: ≥80% viability and ≥80% confluency (plated cells)
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