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Oligonucleotide Synthesis for Therapeutics |
Accelerate your oligonucleotide synthesis and development with a wide variety of Thermo Fisher Scientific phosphoramidites and tools. Our expert product support team is available to guide your screening, development, manufacturing and beyond, ensuring you have the most efficient and reproducible path to commercialization. Take your oligonucleotide synthesis project from early research to preclinical candidate with a single supplier that understands your needs every step of the way.
Oligonucleotide therapeutics are a new class of drugs comprised of short strings of synthetic nucleotides. Oligonucleotide synthesis is a chemical pathway sequentially incorporating phosphoramidites to grow specific sequences of modified and unmodified DNA or RNA. The resulting molecules can then be used in a variety of applications including forensic and diagnostic assays (PCR, qPCR, and other platforms), nucleic acid therapeutics (RNAi, antisense, aptamer, CRISPR), and basic research (Figure 1).
Figure 1. A schematic diagram outlines the phosphoramidite oligonucleotide solid phase synthesis method.
Thermo Fisher Scientific offers a range of phosphoramidites and solid supports for oligonucleotide synthesis:
Custom chemistries (e.g., triphosphate, phosphoramidites, and custom organic), customized QC, and packaging options including filling and labeling are available.
Originally designed specifically to meet the needs of oligonucleotide synthesis and development, TheraPure phosphoramidites are now important for therapeutics, molecular assay, and gene synthesis applications, which benefit from low levels of critical impurities. Our Thermo Scientific TheraPure phosphoramidites are designed for oligonucleotide manufacturers requiring highly defined impurity profiles and strictly controlled processes.
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dA(Bz) CEP | dC(Bz) CEP | dG(iBu) CEP | dT CEP | |
---|---|---|---|---|
Cat. No. | 27-2030 | 27-2032 | 27-2034 | 27-2036 |
Formula | C47H52N7O7P | C46H52N5O8P | C44H54N7O8P | C40H49N4O8P |
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Click images to enlarge
2′-MOE-A CEP | 2′-MOE-5mC CEP | 2′-MOE-G CEP | 2′-MOE-T CEP | |
---|---|---|---|---|
Cat. No. | 27-1019 | 27-1020 | 27-1022 | 27-1021 |
Formula | C50H58N7O9P | C50H60N5O10P | C47H60N7O10P | C43H55N4O10P |
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2′-Fluoro-Bz-A-CEP | 2′-Fluoro-iBu-G-CEP | TheraPure 2′-Fluoro-Ac-C-CEP | TheraPure 2′-Fluoro-U-CEP | TP 2'-Fluoro-Ac-G-CEP | |
---|---|---|---|---|---|
Cat. No. | 27-1601 | 27-1607 | 27-1604 | 27-1602 | SY5410* |
Formula | C47H51FN7O7P | C44H53FN7O8P | C41H49N5FO8P | C39H46FN4O8P | C42H49FN7O8P |
* For more specification details, contact us.
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2′-OMe-Bz-A-CEP | 2′-OMe-U-CEP | 2′-OMe-iBu-G-CEP | 2′-OMe-Ac-C-CEP | |
---|---|---|---|---|
TheraPure phosphoramidites' Cat. No. | 27-2042 | 27-2044 | 27-2046 | 27-2043 |
Formula | C48H54N7O8P | C40H56N7O9P | C45H56N7O9P | C42H52N5O9P |
With over 4 decades of engineered nucleic acid manufacturing experience, phosphoramidites combine our expertise in protection chemistries, modifications, manufacturing scale-up, and innovative purification strategies. All catalog (and custom) phosphoramidites are manufactured in our state-of-the-art Milwaukee, WI manufacturing facility, with an ISO 9001:2015 certified quality management system.
Processes are monitored from material procurement through final QC and product release, providing batch-to-batch reproducibility. Our rigorously controlled manufacturing processes minimize levels of reactive P(III) impurities which could be incorporated into the oligonucleotide during synthesis and result in the accumulation of by-products. TheraPure phosphoramidites from Thermo Fisher Scientific are designed for pharmaceutical manufacturers looking for core building blocks to use in research and manufacturing. These reagents are crafted in facilities with thoroughly documented quality systems and audited by accredited registrars.
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For Research Use Only. Not for use in diagnostic procedures.