Invitrogen Qubit fluorometric quantitation comprises the easy-to-use Invitrogen Qubit 4 Fluorometer and sensitive, specific Invitrogen Qubit quantitation assays to measure RNA quality and quantity, quantitate RNA, ssDNA, dsDNA and protein for applications that require sensitive, specific and accurate measurement.

Designed for your precious samples and high-value applications

The Invitrogen Qubit 4 Fluorometer is designed to quickly and specifically quantify DNA or RNA. In addition, with the newest version of the Qubit Fluorometer, you can now easily measure RNA integrity and quality. It is easier than ever to find out for sure if you have enough DNA or RNA for your experiment.

Request a quote or demoOrder now

Qubit Flex Fluorometer

Get the one-of-a-kind 8-sample fluorometer for flexible throughput plus all the benefits of the Qubit 4 Fluorometer.

Qubit 4 Fluorometer

Get accurate data in a few seconds with touch screen–controlled operation and your choice of assays.

Qubit Assays

Find reagent kits for DNA, RNA, and protein quantification, even for dilute samples.

Sensitive, accurate biomolecule quantification

Qubit Fluorometers detects fluorescent dyes that are specific to the target of interest. These fluorescent dyes emit only when bound to the target molecules, even at low concentrations (Figure 1). Both the Qubit 4 Fluorometer and the Qubit Flex Fluorometer are orders of magnitude more sensitive than UV absorbance, which measures anything absorbing at 260 nm—DNA, RNA, protein, free nucleotides, or excess salts. Moreover, UV spectrophotometry often does not have the sensitivity to accurately measure low concentrations of DNA and RNA (Figure 2). With a Qubit Fluorometer, your research is enhanced by more accurate measurements because the dyes in the Invitrogen Qubit assay kits fluoresce only when bound to the selected molecule—DNA, RNA, or protein—in your sample. This allows you to avoid repeating work due to inaccurate measurements.

Performance of the Qubit dsDNA HS Assay

Figure 1. Performance of the Qubit dsDNA HS Assay. The Invitrogen Qubit dsDNA HS Assay has a linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA.

Accuracy and precision of the Qubit Quantitation Platform

Figure 2. Qubit platforms give sensitive, accurate and precise measurements compared to UV absorbance instruments. Ten replicates of lambda DNA at concentrations from 0.01 to 10 ng/μL were assayed using the Qubit dsDNA HS Assay on the Qubit Fluorometer according to the standard kit protocol. The same concentrations of DNA were measured in 10 replicates using a microvolume spectrophotometer, and results were compared for both accuracy and precision. Each bar represents the average of 10 replicates. Error bars represent the standard deviations of the 10 replicates. The concentrations indicated are the concentrations of DNA in the starting samples, before dilution in the Qubit assay tubes.


Keeping pace with your research

Quantitate DNA, RNA, or Protein with the new Qubit 4 Fluorometer

The Qubit quantification platform continues to evolve with you, offering:

  • Qubit assay dyes bind selectively to DNA, RNA, or protein, making it more sensitive than UV absorbance
  • Uses as little as 1 μL of sample, even with very dilute samples
  • Fast, reliable detection of degraded RNA with the new Invitrogen Qubit RNA IQ Assay
  • New integrated reagent calculator to quickly generate working solution calculations
  • Flexible options for exporting results: Wi-Fi, USB drive, or direct connection with a USB cable
     
qubit-dsdna-assay-kit-thumb

Looking for Qubit assays?

See the full selection of Invitrogen Qubit assays to accurately detect DNA, RNA and protein using Qubit fluorometers.

Shop Qubit assays

Ordering information