Fast, accurate DNA, RNA, and protein quantification, one sample at a time

The Thermo Scientific NanoDrop One/OneC Spectrophotometer is a compact, stand-alone UV-visible spectrophotometer for measurement of highly concentrated samples across a wide variety of analytes without the need for dilution. A glove-compatible touchscreen offers easy access to preloaded software applications and enables purity and concentration assessments in seconds. Built-in Acclaro Sample Intelligence technology helps identify and correct for the contaminants present. Make informed decisions on sample quality and improve your success in downstream applications with NanoDrop technology.


Choose the NanoDrop One instrument for pedestal-only sampling or the NanoDrop OneC instrument for pedestal or cuvette sampling and an extended dynamic range.

Watch the NanoDrop One Spectrophotometer in action

NanoDrop One and Oneᶜ Spectrophotometer features

Our flagship NanoDrop One and OneC spectrophotometers share common features with other members of the NanoDrop family:

Pioneering pedestal technology

Simply pipet a drop of your DNA, RNA, or protein sample on the pedestal and pull down the arm; results appear in seconds


Advanced sample intelligence

By analyzing absorbance across the full spectrum, Acclaro Sample Intelligence software can identify contaminants and correct quantitation results before you use them in downstream applications


Rapid time to results

Results appear within seconds of initiating a measurement, and Auto-Blank and Auto-Measure settings can further streamline your workflow

Tiny sample sizes

Requires only 1–2 µL of sample without dilution, even for highly concentrated samples


Differentiates DNA from RNA

Acclaro software uses full-spectrum data to tease apart DNA from RNA and calculate corrected nucleic acid concentrations


Your dependable lab partner

Since their introduction in 2001, NanoDrop spectrophotometers have become indispensable in many labs, accumulating over 55,000 scientific citations

In addition, the NanoDrop One/OneC Spectrophotometer offers indispensable features that make it a reliable, easy-to-use laboratory workhorse for many sample and assay types, including regulated environments. To learn more, see the sections below.

Expanded dynamic range

Measure up to 27,500 ng/µL dsDNA or 400 mg/mL IgG without dilution


Streamlined multi-sample processing

With instant Auto-Measure and Auto-Blank capabilities


Cuvette option

Choose the NanoDrop One instrument for sample pedestal only or the NanoDrop OneC instrument for both pedestal and cuvette


Advanced connectivity

Connect, export, print, and view data remotely


Optional security software for compliance

Security Suite software aids compliance with FDA 21 CFR Part 11 electronic recordkeeping regulations

Wide spectral range

Measure the full UV-Vis spectrum from 190–850 nm, allowing a range of assays as shown in the table below


Routine and custom embedded applications

DNA, RNA, and protein quantification, full spectral data, purity ratios (A260/A280, A260/A230), and more


Local or PC control software

Operate your instrument from the glove-compatible touchscreen or your Ethernet-connected PC, including custom methods and data export capabilities


Onboard integrated Learning Center

A repository of technical support documents and educational animations at your fingertips

Sample or analysis type



205 nm


260 nm

Purified protein

280 nm

Toxicology assays and industrial dyes

490 nm

Gold nanoparticles

520 nm

Colorimetric protein assay: BCA

562 nm

Colorimetric protein assay: Bradford

595 nm

Colorimetric protein assay: Modified Lowry

650 nm

Colorimetric protein assay: Pierce 660

660 nm

Optical density measurements

600 nm

Powerful, flexible UV-Vis spectrophotometer operating and compliance software

The high-definition, glove-compatible touchscreen interface of NanoDrop One/OneC spectrophotometers give you complete onboard control of your instrument. The user-friendly interface includes custom methods and data export capabilities. An integrated, onboard Learning Center maintains an archive of technical support documents and educational animations at your fingertips.


In addition to the onboard touchscreen interface, free NanoDrop One/OneC PC Control software allows user control of the instrument from an Ethernet-connected PC. 


For regulated labs, optional Thermo Scientific Security Suite for NanoDrop One/OneC allows instrument use in a 21 CFR Part 11 compliant environment. This module helps to maintain the records required for user account access, audit trails, electronic signatures, automated data back-up, and more. 

Onboard touchscreen interface. The ergonomic, standalone NanoDrop One/OneC instruments offer onboard control via a high-definition, glove-compatible touchscreen.

Acclaro Sample Intelligence Technology

Thermo Scientific Acclaro Sample Intelligence Technology software (included with NanoDrop One/OneC and NanoDrop Eight spectrophotometers) analyzes absorbance across the whole UV-Vis spectrum, not just wavelengths specific to nucleic acids and proteins. It then applies powerful chemometric algorithms to find and correct for contamination in your samples. You get more reliable downstream assay results and avoid potential rework with this efficient, breakthrough capability.

Next-generation quality assessment using Acclaro software

Chemometric algorithms allow NanoDrop instruments to identify and correct for specific contaminants, taking quality assessment to the next level.

Acclaro Sample Intelligence software can even use full-spectrum data to tease apart DNA from copurified RNA and calculate corrected DNA concentrations.

Verify sample quality in seconds

Identify sample contaminants and obtain corrected concentration results


Identify contaminants that absorb in the analysis region

Determine absorbances from known contaminants like protein or phenol in dsDNA or RNA samples or nucleic acids or phenol in protein samples 


Rely on accurate results

Help ensure measurement integrity with embedded sensor and digital image analysis

Access information through alerts

Receive instant feedback about sample quality with on-demand technical support and guided troubleshooting


Analyze the entire spectrum to expose other contaminants

Detect the presence of guanidine HCl and guanidinium isothiocyanate in nucleic acid samples, dsDNA in RNA, or RNA in dsDNA

Advanced connectivity for data storage, printing, and sharing

Improve your laboratory’s productivity and expand your collaborations with connected laboratory instruments that accelerate your research. With 24/7 access to the Thermo Fisher Connect cloud-based platform using the free NanoDrop Cloud App, you can securely upload, store, view, and share your data anytime, anywhere, from any device. 


Or, when you want to work at your desk, you can use NanoDrop One PC Control software to view and analyze your data or explore Acclaro support features on your PC. Then, when your results are ready, you can print directly or seamlessly transfer results to a PC or the Connect platform using a USB, *Wi-Fi, or Ethernet connection. 


Wi-Fi model not available in certain regions; consult your local NanoDrop distributor.

Choose the pedestal-only or pedestal/cuvette option

Although both models in the NanoDrop One family have the pioneering microvolume sample retention capability of our pedestal, the NanoDrop OneC instrument includes a built-in cuvette capability. The cuvette position adds experimental flexibility and increases the dynamic range. Combining the pioneering sample-retention system with cuvette capability accommodates both low and high concentrations (0.2–27,500 ng/µL dsDNA and 0.006–820 mg/mL BSA).


Use cuvettes to measure dilute samples and optical density of bacterial cultures or to perform kinetics experiments. The cuvette position can be used with the arm up or down and includes temperature control and stirring features.

NanoDrop One and Oneᶜ Spectrophotometer customer stories

NanoDrop One Spectrophotometer - Scientist Feedback, University of Cambridge - Robyn Hardisty

Watch this NanoDrop One testimonial video with a University of Cambridge scientist who studies the chemical biology of nucleic acids.

NanoDrop One UV-Vis Spectrophotometer Review – Alyssa Grantham

See what an experienced, young scientist working on amyotrophic lateral sclerosis (ALS) research has to say about her first impressions of the NanoDrop One instrument.

Whether you are ...

starting your career,

developing your career,

or progressing your career,

NanoDrop One UV-Vis spectrophotometers are with you every step of the way.
Peter Tao – Research Assistant, Neurobiology laboratory, Ocular degeneration research Alyssa Grantham – Research Assistant, Neurobiology laboratory, Amyotrophic lateral sclerosis disease research Angela Botes Ph.D - Postdoctoral Associate, Biology laboratory, Metabolic engineering and synthetic biology research
Peter Tao Alyssa Grantham Angela Botes
"The compactness of the instrument most definitely stands out from everything else. Well, a lot of things stand out about it but the compactness is the best part for me because coming from a lab where we don't have that much space we want more of it…” "…the auto measure makes sampling faster, easier…I try to figure out the fastest way to read samples when I'm short on time. So I'll get one sample ready while one sample's reading. So I can just set it up, I don’t have to click a button to set GO, I just put it (the pedestal arm) back down and my next sample is ready. "I tend to forget what the (purity) ratio should be. I have a rough idea of what the ratio with protein contamination should be...and this is very helpful to have that extra feature (educational animations) that will correct the concentration for you so what you think your DNA concentration is, is what (the) actual DNA concentration is. That's gonna save a lot of time.”

NanoDrop One and Oneᶜ Spectrophotometer specifications





Minimum Sample Size

1 μL

Sample Number


Limit of Detection

Pedestal: 2 ng/μL (dsDNA), 0.06 mg/mL (BSA)

Cuvette (NanoDrop OneC only): 0.2 ng/μL (dsDNA), 0.006 mg/mL (BSA)

Maximum Concentration

Pedestal: 27,500 ng/μL (dsDNA), 820 mg/mL (BSA)

Cuvette (NanoDrop OneC only): 75 ng/μL (dsDNA)



0.030 to 1.0 mm automatically adjusting

Light Source

Xenon flash lamp

Detector Type

2048-element CMOS linear image sensor

Wavelength Range

190–850 nm

Wavelength Accuracy

±1 nm

Spectral Resolution

≤1.8 nm (FWHM at Hg 254 nm)

Typical Measurement Repeatability

0.002 A (1.0 mm path) or 1% CV, whichever is greater

Absorbance Accuracy1

3% (at 0.97 A, 302 nm)

Absorbance Range (10 mm equivalent)

Pedestal: 0–550A

Cuvette (NanoDrop OneC only): 0–1.5A

Measurement and Data Processing Time

8 seconds

21 CFR Part 11 Compliance

Yes, optional


Instrument Control

Built-in touchscreen or PC Control with free PC software

Footprint (W × D × H)

20 × 25.4 × 32.3 cm (8 × 10 × 12.7 in.)


3.6 kg

Sample Pedestal

303 stainless steel and quartz fiber

Cuvette Position

Optional (with stirring) (NanoDrop OneC only)

Operating Voltage

12 V (DC)

Operating Power Consumption

12–18 W


Instrument Control

Built-in touchscreen

Applications Support

Nucleic Acid A260, A260/A280, A260/A230, Labeled Nucleic Acids, Microarray, dsDNA, ssDNA, RNA, Oligo DNA/Oligo RNA, RNA/DNA Deconvolution, Custom Factor; Protein A280 and A205, Protein Pierce 660, Protein Bradford, Protein BCA, Protein Lowry, Proteins & Labels. Protein Editor; Dye Editor, OD600, Kinetics, UV-Vis, and Custom Methods

Language Support

Chinese, French, German, Japanese, Korean, Polish, Spanish, English

Accessory Support

DYMO® LabelWriter® 550 Turbo printer, Bluetooth keyboard, mouse, and barcode reader

Software Compatibility

Standalone, Local Control software

NanoDrop One PC Control (free download)

Thermo Scientific Security Suite (purchase separately)
PC Software: Windows® 10 Professional (64 bit)


Three USB-A ports, Ethernet, Bluetooth, and Wi-Fi*

Advanced Connectivity

NanoDrop One Cloud Application2

Thermo Fisher Connect Platform

1 Absorbance expressed as Abs/mm measured at 25 °C.

2 Visit for details.

NanoDrop One and Oneᶜ Spectrophotometer ordering information