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Fast, accurate DNA, RNA, and protein quantification, one sample at a time
Thermo Scientific NanoDrop Ultra Spectrophotometers and Fluorometers are compact, stand-alone UV-visible spectrophotometers (select models also function as fluorometers) for measurement of highly concentrated samples across a wide variety of analytes without the need for dilution. A glove-compatible touchscreen offers easy access to preloaded software applications and enables purity and concentration assessments in seconds. Built-in Acclaro Sample Intelligence Technology helps identify and correct for the contaminants present. Make informed decisions on sample quality and improve your success in downstream applications with NanoDrop technology.
NanoDrop Ultra instruments introduce a suite of innovative features designed to enhance your laboratory workflow. Key highlights include an integrated qPCR calculator that precisely determines the sample volume required for downstream applications, and the optional Acclaro Pro software, which incorporates an advanced algorithm for highly accurate quantification of concentrated samples. Additionally, a diagnostic scheduler alerts you when a diagnostic test is due, ensuring consistent performance. These, along with numerous other cutting-edge features, make NanoDrop Ultra instruments an indispensable tool for modern laboratories.
Watch the NanoDrop Ultra Spectrophotometer in action
Choose the NanoDrop Ultra instrument for pedestal-only absorbance sampling, NanoDrop UltraC instrument for pedestal or cuvette absorbance sampling, NanoDrop Ultra FL instrument for pedestal-only absorbance and fluorescence sampling, or the NanoDrop UltraC FL instrument for pedestal absorbance and fluorescence sampling and cuvette absorbance sampling.
NanoDrop Ultra spectrophotometers and fluorometers features
Our flagship NanoDrop Ultra spectrophotometers and fluorometers share common features with other members of the NanoDrop family:
Pioneering pedestal technology
Simply pipet a drop of your DNA, RNA, or protein sample on the pedestal and pull down the arm; results appear in seconds
Advanced sample intelligence
By analyzing absorbance across the full spectrum, Acclaro Sample Intelligence software can identify contaminants and correct quantitation results before you use them in downstream applications
Rapid time to results
Results appear within seconds of initiating a measurement, and Auto-Blank and Auto-Measure settings can further streamline your workflow
Streamlined multi-sample processing
With instant Auto-Measure and Auto-Blank capabilities
Tiny sample sizes
Requires only 1–2 µL of sample without dilution, even for highly concentrated samples
Differentiates DNA from RNA
Acclaro software uses full-spectrum data to tease apart mammalian DNA from RNA and calculate corrected nucleic acid concentrations
Your dependable lab partner
Since their introduction in 2001, NanoDrop spectrophotometers have become indispensable in many labs, accumulating over 55,000 scientific citations
In addition, the NanoDrop Ultra spectrophotometers and fluorometers offer indispensable features that make it a reliable, easy-to-use laboratory workhorse for many sample and assay types, including regulated environments. To learn more, see the sections below.
Expanded dynamic range
Measure from 1 ng/µL up to 27,500 ng/µL dsDNA or 0.02 mg/mL to 400 mg/mL IgG without dilution.
Optional Acclaro Pro software for accurate high concentration measurements
With the use of the optional Acclaro Pro software obtain absorbance measurements within 5% error up to 550 Abs (400 mg/mL IgG or 22,000 ng/µL ssDNA).
Diagnostic Scheduler
Use the built-in Diagnostic Schedule application to alert you when a diagnostic test is overdue, ensuring consistent performance of the instrument.
Cuvette option
Choose the NanoDrop Ultra or Ultra FL instrument for sample pedestal only or the NanoDrop UltraC or UltraC FL instrument for both pedestal and cuvette to expand the limit of detection to 0.2 ng/µL dsDNA or 0.006 mg/mL IgG.
Advanced cloud connectivity
Export data directly to popular Cloud storage services like Microsoft OneDrive, Google Drive, and Thermo Fisher Connect.
Optional on-board SciVault 2 software for compliance
On-board Thermo Scientific SciVault 2 Software aids compliance with FDA 21 CFR Part 11 electronic recordkeeping regulations with subsequent seamless integration into your LIMS environment using RESTful API directly on the local instrument. SciVault 2 software can also be installed on a connected computer if desired.
All-in-one absorbance and fluorescence analysis
The NanoDrop Ultra FL and NanoDrop UltraC FL instruments are capable of both UV-Vis spectroscopy and fluorometry. The capable NanoDrop Ultra dsDNA (BR/HS) and RNA (HS) Fluorescence Assays allow for a more sensitive quantification of dsDNA and RNA and can detect down to 0.2 ng/µL dsDNA.
Wide spectral range
Measure the full UV-Vis spectrum from 190–850 nm, allowing a range of assays as shown in the table below
Routine and custom embedded applications
DNA, RNA, and protein quantification, full spectral data, purity ratios (A260/A280, A260/A230), and more
Local or PC control software
Operate your instrument from the glove-compatible touchscreen or your USB-connected PC, including custom methods and data export capabilities
Integrated qPCR Recipe Calculator
Fully integrated qPCR recipe calculator to save time, optimize efficiency, and achieve reliable downstream results with precise reaction setup.
Elevated Sample Insight
Software with the ability to differentiate between DNA and RNA for samples of mammalian, plant, and bacteria origin.
Sample or analysis type |
Technique (Wavelength) |
Peptides |
Absorbance (205 nm) |
DNA and RNA |
Absorbance (260 nm) and Fluorescence |
Purified protein |
Absorbance (280 nm) |
Toxicology assays and industrial dyes |
Absorbance (490 nm) |
Gold nanoparticles |
Absorbance (520 nm) |
Colorimetric protein assay: BCA |
Absorbance (562 nm) |
Colorimetric protein assay: Bradford |
Absorbance (595 nm) |
Colorimetric protein assay: Modified Lowry |
Absorbance (650 nm) |
Colorimetric protein assay: Pierce 660 |
Absorbance (660 nm) |
Optical density measurements |
Absorbance (600 nm) |
Powerful, flexible UV-Vis spectrophotometer and fluorometer operating and compliance software
The high-definition, glove-compatible touchscreen interface of NanoDrop Ultra spectrophotometers and fluorometers give you complete onboard control of your instrument. The user-friendly interface includes stom methods, automated software update notifications, data export to Cloud capabilities, and many other great features. An integrated, onboard Learning Center maintains an archive of technical support documents and troubleshooting tips to help guide and inform you when working with more challenging samples.
In addition to the onboard touchscreen interface, free NanoDrop Ultra PC Control software allows user control of the instrument from a USB-connected PC.
For regulated labs, optional SciVault 2 Software for NanoDrop Ultra allows instrument use in a 21 CFR Part 11 compliant environment. This module, which no longer requires the use of a connected computer, helps to maintain user roles, the records required for user account access, audit trails, electronic signatures, automated data back-up, and more.
Onboard touchscreen interface. The ergonomic, standalone NanoDrop Ultra instruments offer onboard control via a high-definition, glove-compatible touchscreen.
Integrated qPCR recipe calculator
Obtaining a sample concentration is just the beginning – calculating how much sample goes into your downstream reaction can be tedious. Use the built-in library of kits, or add your favorite, to let the software run the math for you. The qPCR recipe calculator suggest how to prepare the reaction based on your sample’s concentration.
Acclaro Sample Intelligence Technology
Thermo Scientific Acclaro Sample Intelligence Technology software (included with NanoDrop Ultra spectrophotometers and fluorometers and NanoDrop Eight spectrophotometers) analyzes absorbance across the whole UV-Vis spectrum, not just wavelengths specific to nucleic acids and proteins. It then applies powerful chemometric algorithms to find and correct for contamination in your samples. You get more reliable downstream assay results and avoid potential rework with this efficient, breakthrough capability.
Next-generation quality assessment using Acclaro software
Chemometric algorithms allow NanoDrop instruments to identify and correct for specific contaminants, taking quality assessment to the next level.
Acclaro Sample Intelligence software can even use full-spectrum data to tease apart DNA from copurified RNA and calculate corrected DNA concentrations for samples of mammalian, plant, and bacteria origin.
Verify sample quality in seconds
Identify sample contaminants and obtain corrected concentration results
Identify contaminants that absorb in the analysis region
Determine absorbances from known contaminants like protein or phenol in dsDNA or RNA samples or nucleic acids or phenol in protein samples
Rely on accurate results
Help ensure measurement integrity with embedded sensor and digital image analysis
Access information through alerts
Receive instant feedback about sample quality with on-demand technical support and guided troubleshooting
Analyze the entire spectrum to expose other contaminants
Detect the presence of guanidine HCl and guanidinium isothiocyanate in nucleic acid samples, dsDNA in RNA, or RNA in dsDNA
Advanced connectivity for data storage, printing, and sharing
Improve your laboratory’s productivity and expand your collaborations with connected laboratory instruments that accelerate your research.
Features include:
- Export to the cloud – Export data directly to popular cloud storage services like Microsoft OneDrive, Google Drive, and Thermo Fisher Connect.
- Direct LIMS integration – Send data directly into your LIMS with the assistance of an integrated RESTful API.
- View data your way – Customize and preview the information included in the data file prior to export to ensure it meets any downstream needs. – Export data directly to popular cloud storage services like Microsoft OneDrive, Google Drive, and Thermo Fisher Connect.
- Direct LIMS integration – Send data directly into your LIMS with the assistance of an integrated RESTful API.
- View data your way – Customize and preview the information included in the data file prior to export to ensure it meets any downstream needs.
Or, when you want to work at your desk, you can use NanoDrop Ultra PC Control software to view and analyze your data or explore Acclaro support features on your PC. Then, when your results are ready, you can print directly or seamlessly transfer results to a PC or a connected Cloud platform using a USB, *Wi-Fi, or Ethernet connection.
Choose the pedestal-only or pedestal/cuvette option
Although all models in the NanoDrop Ultra family have the pioneering microvolume sample retention capability of our pedestal, the NanoDrop UltraC and NanoDrop UltraC FL instruments include a built-in cuvette capability. The cuvette position adds experimental flexibility and increases the dynamic range. Combining the pioneering sample-retention system with cuvette capability accommodates both low and high concentrations (0.2–27,500 ng/µL dsDNA and 0.006–820 mg/mL BSA).
Use cuvettes to measure dilute samples and optical density of bacterial cultures or to perform kinetics experiments. The cuvette position can be used with the arm up or down and includes temperature control and stirring features.
NanoDrop spectrophotometer customer stories
Whether you are ...
starting your career, |
developing your career, |
or progressing your career, |
| NanoDrop UV-Vis spectrophotometers are with you every step of the way. | ||
| Peter Tao – Research Assistant, Neurobiology laboratory, Ocular degeneration research | Alyssa Grantham – Research Assistant, Neurobiology laboratory, Amyotrophic lateral sclerosis disease research | Angela Botes Ph.D - Postdoctoral Associate, Biology laboratory, Metabolic engineering and synthetic biology research |
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| Peter Tao | Alyssa Grantham | Angela Botes |
| "The compactness of the instrument most definitely stands out from everything else. Well, a lot of things stand out about it but the compactness is the best part for me because coming from a lab where we don't have that much space we want more of it…” | "…the auto measure makes sampling faster, easier…I try to figure out the fastest way to read samples when I'm short on time. So I'll get one sample ready while one sample's reading. So I can just set it up, I don’t have to click a button to set GO, I just put it (the pedestal arm) back down and my next sample is ready. | "I tend to forget what the (purity) ratio should be. I have a rough idea of what the ratio with protein contamination should be...and this is very helpful to have that extra feature (educational animations) that will correct the concentration for you so what you think your DNA concentration is, is what (the) actual DNA concentration is. That's gonna save a lot of time.” |
NanoDrop Ultra spectrophotometers and fluorometers specifications
| Category | Attribute | Description |
| Pedestal Spectrophotometric | Minimum Sample Size | 1 μL |
| Sample Number | 1 | |
| Limit of Detection | 1 ng/μL (dsDNA), 0.06 mg/mL (BSA) | |
| Maximum Concentration | 27,500 ng/μL (dsDNA), 820 mg/mL (BSA) | |
| Pathlength(s) | 0.030 to 1.0 mm automatically adjusting | |
| Measurement Repeatability1 | 0.002 A (1.0 mm path) or 1% CV, whichever is greater | |
| Photometric Range | 0.02 - 550 Abs (10 mm equivalent) | |
| Photometric Accuracy2 | 3% at 0.97 A, 302 nm, 23 ± 2C° | |
| Measurement & Data Processing Time | ≤ 7 sec | |
| Acclaro Pro - Photometric Accuracy | 5% up to 550 Abs | |
| Acclaro Pro - Measurement & Data Processing Time | ≤ 30 sec | |
| Cuvette Spectrophotometric | Limit of Detection | 0.2 ng/μL (dsDNA), 0.006 mg/mL (BSA) |
| Maximum Concentration | 75 ng/μL (dsDNA), 2 mg/mL (BSA) | |
| Photometric Range | 0.004 - 1.5 Abs | |
| Pathlength(s) | 10 mm, 5 mm, 2 mm, 1 mm | |
| Beam Height (Z-Height) | 8.5 mm | |
| Temperature Control | 37.0 °C ± 0.5 °C | |
| Strirring | 9 speeds | |
| Absorbance-Based Optics | Light Source | Xenon flash lamp |
| Detector Type | 2048-element CMOS linear image sensor | |
| Wavelength Range | 190–850 nm | |
| Wavelength Accuracy | ±1 nm | |
| Spectral Resolution | ≤1.8 nm (FWHM at Hg 254 nm) | |
| Pedestal Fluorescence | Minimum Sample Size | 2 μL |
| Limit of Detection | Assay Specific3 | |
| Maximum Concentration | ||
| Measurement & Data Processing Time | ≤ 20 sec for Blue LED; ≤ 40 sec for Red LED | |
| Fluoresence-Based Optics | LEDs | Blue (max ~470 nm) and Red (max ~635 nm) |
| Excitation Filters | Blue (430-495 nm) and Red (600-645 nm) | |
| Characteristics | Footprint (W × D × H) | 32 × 18 × 28 cm (12.6 × 7.1 × 11.0 in.) |
| Weight | 4.1 kg | |
| Sample Pedestal | 303 stainless steel and quartz fiber | |
| Cuvette Position | Optional (with stirring) (NanoDrop UltraC and NanoDrop UltraC FL only) | |
| Operating Voltage | 12 V (DC) | |
| Power Consumption | 7W at Idle; 15W at Working Conditions | |
| Instrument Control | Built-in touchscreen or PC control with free PC software | |
| 21 CFR Part 11 Compliance | Yes, optional software for use on either PC or local instrument | |
| System | Applications Support | Nucleic Acid A260, A260/A280, A260/A230, Labeled Nucleic Acids, Microarray, dsDNA (Pro), ssDNA (Pro), RNA (Pro), Oligo DNA/Oligo RNA (Pro), RNA/DNA Deconvolution, Custom Factor (Pro); Protein A280 (Pro) and A205, Protein Pierce 660, Protein Bradford, Protein BCA, Protein Lowry, Proteins & Labels, dsDNA Fluorescence, RNA Fluorescence, Fluorometer. Protein Editor; Dye Editor, Kit Editor, OD600, Kinetics, UV-Vis, and Custom Methods |
| Language Support | Chinese, French, German, Japanese, Korean, Polish, Spanish, English, Italian | |
| Accessory Support | DYMO® LabelWriter® 550 Turbo printer, Bluetooth keyboard, mouse, and barcode reader | |
| Software Compatibility | Standalone, local control software | |
| NanoDrop Ultra PC control (free download) | ||
| Thermo Scientific SciVault 2 software (purchase separately) | ||
| PC Software: Windows® 10/11 Professional (64 bit) Acclaro Pro software (purchase seperately) |
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| Connectivity | 2x USB-A Ports, 1x USB-C Port, Ethernet, Bluetooth, Wi-Fi | |
| Advanced Connectivity | Google Drive | |
| Microsoft OneDrive | ||
| Thermo Fisher Connect Platform |
- SD of 10 individual samples measured at 0.97 A
- Absorbance expressed at Abs/mm at 25°C
- See NanoDrop Ultra dsDNA Broad Range (BR), NanoDrop Ultra dsDNA High Senstivitiy (HS), and NanoDrop Ultra RNA High Sensitivity (HS) Fluoresence Assays for more information.
NanoDrop Ultra spectrophotometers and fluorometers ordering information
For Research Use Only. Not for use in diagnostic procedures.