Plant DNA & RNA Isolation
Plant DNA Isolation has unique challenges that require kits specifically designed to deal with carbohydrates, phenolics, and other compounds abundant in plant tissues. Plant cell walls can be very difficult to disrupt, and lysates often contain significant amounts of compounds such as tannins, phenolics, and complex polysaccharides that can affect DNA and RNA quality and inhibit downstream reactions. Life Technologies offers an expansive portfolio of kits, reagents, and devices for the isolation, purification, analysis, and quantitation not only for plant genomic DNA, but also for total RNA, mRNA, and microRNA or transcriptome RNA.
Which Plant DNA Isolation Kit is Right for You?
| Bulk buffers for crude extraction of DNA and RNA from plant cells (*) | DNA extraction and TaqMan® chemistry-based genotyping combined, <1h to results | The most efficient lysis for the largest amount of tissue | Most suitable for screening and GMO testing due to automation capabilities | Slica-based method for low abundance plant DNA samples | Magnetic beads designed to handle low-abundance plant DNA samples | ||||||
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| Starting Material | ≥ 100 mg | 2-3mm punch | ≥ 100 mg | 50-100 mg | 100 mg | 5-10 mg | |||||
| Yield | varies based on starting material | varies based on starting material | varies based on starting material | up to 7 µg | up to 14.6 µg | up to 14.6 µg | |||||
| Protocol time | <15 mins | 5 mins | <60 mins | <15 mins | <40 mins | <40 mins | |||||
| Plant sample types | Customer-validated for DNA on a broad range of plant tissues for SNP genotyping as well as corn flour for GMO testing | Works with broad rangeof plant tissues and starting material (leaf punch, needle, seed chips) | Broad range of plant tissues (alfalfa and sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass and Arabidopsis thaliana leaves) | Some of the many species and tissues are canola, corn, cotton, sunflower, barley, rice (seeds); tomato, cucumber, lettuce, banana (leaves); rose, magnolia (flower); Acer, sycamore, grass, etc. | Chloroplasts and broad range of plant tissues (alfalfa and sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass and Arabidopsis thaliana leaves) | Chloroplasts and broad range of plant tissues (alfalfa and sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass & Arabidopsis thaliana leaves) | |||||
| Isolation technology | Bulk lysis buffer | Lysis solution | Organic extraction | ChargeSwitch® Chemistry, magnetic beads | Silica spin column | Magnetic beads | |||||
| Downstream applications | PCR, real-time PCR | Real-time PCR | All | All | All | All | |||||
| Automatable | no | yes | no | yes | no | yes | |||||
| High throughput compatibile | yes | yes | yes | yes | no | yes | |||||
| High throughput/
automation options | yes | yes | yes | ChargeSwitch® gDNA Plant 960 Kit | 96-well plate | 50 preps
96 preps |
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| Cost/prep | $0.41 | $1.29 | $1.81 | $2.56 | $2.46 | $2.82 | |||||
| Kit sizes available | 250 mL | 100 preps
400 preps 4000 preps | 100 mL | 96 preps
960 preps | 50 preps
4 X 96 preps | 50 preps
96 preps |
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| Order now | Order now | Order now | Order now | Order now | Order now |
(*)The Total RNA Lysis solution was part of the discontinued ABI PRISM® 6100 Nucleic Acid PrepStation and is our cheapest and quickest methodology for plant DNA extraction without the use of organic solvents.
Which Plant RNA Isolation Kit is Right for You?
| Great for difficult samples (conifer tissue & seeds) | Rapid & fully automated | Quick & easy to use | Efficient recovery of microRNA & small RNA | ||||
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| RNA types isolated | large RNA molecules only | large RNA molecules only | large RNA molecules only | Small & large RNA molecules | |||
| Prep time | 60 minutes | <45 minutes | <20 minutes | 30 minutes | |||
| Amount of starting material | Up to 1 g | Up to 10 mg | <250 mg | 0.5 to 250 mg | |||
| Isolation method | High purity organic extraction
(requires alcohol precipitation) | Scalable, flexible format with magnetic beads | Fast, convenient silica column | Highest purity & convenience; includes organic extraction & silica column
(no precipitation required) |
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| High throughput compatible | | ||||||
| Compatible products | Ambion® Plant RNA Isolation Aid
Removes common plant contaminants | Ambion® Plant RNA Isolation Aid
Removes common plant contaminants | Ambion® Plant RNA Isolation Aid
Removes common plant contaminants |
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| Price per prep | $1.36 | $3.00 | $4.50 | $6.65 | |||
| Order Now | Order Now | Order Now | Order Now |
Other Products of InterestIn addition to DNA and RNA isolation products, Life Technologies offers an expansive portfolio of solutions for analysis and quantitation of plant genomic DNA. |
Technical ResourcesDNA RNA |
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