In this collection, protocols for each antibody application are provided along with links to antibodies, reagents, and resources. Learn how to perform each application so quality, consistent results are attained every time.
Western blot (WB) is a technique that separates a mixture of proteins via size exclusion electrophoresis. The target protein is identified using an antibody specific for the target and is visualized as a band via chemiluminescent or fluorescent detection.
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Western blot antibody dilution calculator
Flow cytometry identifies cellular characteristics and expression of proteins in a heterogeneous cell population by flowing one cell at a time past a laser beam.
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Immunohistochemistry (IHC) identifies structures in whole tissue samples. A mixture of antibodies and stains are used to microscopically visualize the cells.
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Download IHC guide
Immunocytochemistry/Immunofluorescence (ICC/IF) identifies cells from samples that are no longer in their natural state. Antibodies are used to microscopically visualize cellular structures.
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Enzyme-linked immunosorbent assay (ELISA) identifies protein concentrations using antibodies and a plate with multiple sample wells. Visualization is performed using colorimetric or fluorescent enzymatic substrates and is represented graphically.
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Download Biomarker guide
Chromatin immunoprecipitation (ChIP) analyzes protein-DNA interactions. The target interactions are identified using antibodies and the DNA is amplified for graphical visualization.
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Download ChIP guide | Protein preparation handbook
Immunoprecipitation (IP) precipitates a target protein from a mixture of proteins using an antibody. Immunoprecipitation can be used in conjunction with other assays or independently.
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Protein preparation handbook