In this collection, protocols for each antibody application are provided along with links to antibodies, reagents, and resources. Learn how to perform each application so quality, consistent results are attained every time.
Western blot (WB) is a technique that separates a mixture of proteins via size exclusion electrophoresis. The target protein is identified using an antibody specific for the target and is visualized as a band via chemiluminescent or fluorescent detection.
Immunocytochemistry/Immunofluorescence (ICC/IF) identifies cells from samples that are no longer in their natural state. Antibodies are used to microscopically visualize cellular structures.
Enzyme-linked immunosorbent assay (ELISA) identifies protein concentrations using antibodies and a plate with multiple sample wells. Visualization is performed using colorimetric or fluorescent enzymatic substrates and is represented graphically.
Immunoprecipitation (IP) precipitates a target protein from a mixture of proteins using an antibody. Immunoprecipitation can be used in conjunction with other assays or independently.
For Research Use Only. Not for use in diagnostic procedures.