- Reliability—Produces conjugates with stable, covalent attachment of superior fluorescent labels
- Convenience—Requires only 10–20 µg of IgG antibody per labeling reaction, regardless of any purity limitations
- Complete—Includes everything required to perform 5 separate labelings
How APEX® Antibody Labeling Kits work
The APEX® Antibody Labeling Kits use a solid-phase labeling technique that captures the IgG antibody on the resin inside the APEX® antibody labeling tip (Figure 1). Any contaminants, including stabilizing proteins or amine-containing buffers, are simply eluted through the tip. After applying the amine-reactive label, a fluorescent IgG conjugate is ready for use in any imaging or flow cytometry application in as little as 2.5 hours with very little hands-on time. The typical yield of labeled antibody using this method ranges from 40–80%.
The APEX® Antibody Labeling Kits includes all reagents required to perform 5 separate labeling reactions of 10–20 μg of IgG antibody with one of Molecular Probes® superior fluorophores or biotin-XX. The biotin-XX provides a 14-atom spacer between the antibody and the biotin moiety.
APEX® Antibody Labeling Kits selection guide
|Fluorophore or biotin label||Ex/Em (nm)||Application||Cat. No.|
|Alexa Fluor® 488||496/519||Fluorescent label for use in imaging or flow cytometry; hapten for signal amplification with anti–Alexa Fluor® 488 antibodies.||A10468|
|Alexa Fluor® 555||555/565||Fluorescent label for use in imaging||A10470|
|Alexa Fluor® 568||578/603||A10494|
|Alexa Fluor® 594||590/617||A10474|
|Alexa Fluor® 647||650/665||Fluorescent label for use in imaging or flow cytometry||A10475|
|Oregon Green® 488||496/524||Fluorescent label for use in imaging or flow cytometry; hapten for signal amplification with anti-fluorescein/Oregon Green® antibodies||A10476|
|Pacific Blue™ dye||416/451||Fluorescent label for use in imaging or flow cytometry||A10478|
|Biotin-XX||NA||Streptavidin, avidin, and NeutrAvidin® binding partner for use in imaging or flow cytometry||A10495|
Find more information about Alexa Fluor® dye and other fluorophores in the Fluorophore Selection Guide
Need to label other antibody amounts?
|IgG amount||Attachment of label||Products|
|<1–20 µg||Site-specific||Zenon® Antibody Labeling Kits|
|<100 µg||Site-specific||SiteClick™ Antibody Labeling Kits|
|10–20 µg||Conventional covalent||APEX® Antibody Labeling Kits|
|20–100 µg||Conventional covalent||Microscale Protein Labeling Kits|
|100 µg||Conventional covalent||Antibody Labeling Kits|
|1 mg||Conventional covalent||Protein Labeling Kits|
|0.5–3 mg||Conventional covalent||SAIVI™ Rapid Antibody Labeling Kits|
BioProbes Journal articles
Molecular Probes Handbook
For Research Use Only. Not for use in diagnostic procedures.