Antibody Purification Kits and Reagents
Purification methods range from very crude to highly specific:
- Crude–precipitation of a subset of total serum proteins that includes immunoglobulins
- General–affinity purification of certain antibody classes (e.g., IgG) without regard to antigen specificity
- Specific–affinity purification of only those antibodies in a sample that bind to a particular antigen molecule
The level of purification necessary to obtain usable antibody depends upon the intended application(s) for the antibody.
We offer a wide selection of agarose beads, affinity columns, and purification kits to purify total or specific antibodies from serum, ascites fluid, or tissue culture supernatant. Our offering includes many varieties of Protein A, Protein G, Protein A/G, and Protein L.
Featured antibody purification categories
Accomplish affinity purification of IgG antibodies from many species utilizing Protein A, Protein G, Protein A/G, or Protein L covalently immobilized onto porous resins (such as beaded agarose) or magnetic beads.
Dynabeads® magnetic separation technology can help meet your immunoprecipitation needs. Dynabeads® magnetic beads are now cited in over 5,200 published articles.
Choose agarose resins, magnetic beads, or other affinity supports that use native or recombinant forms of Protein A, G, A/G, and L to bind and purify IgG and other classes of immunoglobulins (e.g., antibody subclass or animal species). Also find affinity purification products for other types of antibodies, including mouse and human IgM from ascites, and human serum IgA or secretory IgA1.
Choose a chromatography resin or precipitation reagent to purify the main classes of serum immunoglobulins (e.g., IgG, IgM) from serum and ascites fluids. Antibody physicochemical properties are sufficiently different from most other abundant proteins in serum, such as albumin and transferrin that they can be selected and enriched on the basis of these differentiating features.
Thermo Scientific Pierce IgG binding and elution buffers ensure high-yield, non-denaturing antibody purification using IgG affinity purification supports such as Protein A and Protein G agarose beads. Several of these ready-made buffers are also effective for antibody-antigen or protein-protein affinity purification systems.
Antigen-specific antibody purification is accomplished by immobilizing the antigen used for immunization onto a resin or bead for use as an affinity support. Choose from a variety of activated affinity supports (porous resins or magnetic beads) to successful immobilize peptides, proteins and other antigens amino, sulfhydryl, carboxyl, or glycosyl functional groups.
Partner with us to scale-up your process or product development pipeline with bulk quantities of high-quality immunoassay reagents and biochemicals. We offer a variety of compounds, devices, and resins for your large-scale applications.
Please click on the button below to fill out our bulk and custom ordering form.
For Research Use Only. Not for use in diagnostic procedures.