Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein A/G is a recombinant protein expressed in Escherichia coli (50.5 kDa; 6 IgG binding sites) and is recommended for purifying polyclonal IgG from many species. The POROS MabCapture A/G Select Resin is a new product that can be scaled up for bioprocess applications.

We offer a wide selection of base supports and formats to purify antibodies using the Protein A/G ligand. Our portfolio is designed to meet small-scale (screening) up to large-scale (bioprocess) needs.

  • Multiple formats—magnetic agarose beads, loose resins, spin columns and kits, FPLC cartridges, and 96-well filter plates enable antibody purification from microgram to kilogram scales
  • High performance—resins are designed to maximize protein yield and reduce background
  • Economical—pricing is similar to or better than our leading competitors

Choose the right Protein A/G product for antibody purification

Product links in the last row of each column

 Pierce Protein A/G
Magnetic Agarose Beads
Pierce Protein A/G
Agarose
Pierce Protein A/G Plus AgarosePOROS MabCapture A/G Select
Bead or resin size10–40 μm45–165 μm45–165 μm50 μm
Binding capacity≥40 mg rabbit IgG/mL beads≥7 mg human IgG/mL resin≥50 mg human IgG/mL resin≥22 mg/mL human IgG/mL resin
Maximum linear flow rateN/A700 cm/hr700 cm/hr3000 cm/hr
SupportMagnetite-embedded beaded agaroseCrosslinked 6% beaded agaroseCrosslinked 6% beaded agarosePolystyrene-divinylbenzene
Recommended application scalehigh-throughput batchbatch, pilotbatch, pilotbatch, pilot, process
Formats availableloose beadsloose beads, pre-packed spin columns and kits, chromatography cartridgesloose beadsloose beads
Order product(s)7860920421
(loose resin)
89930
(1 mL cartridge)
89931
(5 mL cartridge)
2042382082

For Research Use Only. Not for use in diagnostic procedures.