Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein A/G is a recombinant protein expressed in Escherichia coli (50.5 kDa; 6 IgG binding sites) and is recommended for purifying polyclonal IgG from many species. The POROS™ MabCapture™ A/G Select Resin is a new product that can be scaled up for bioprocess applications.

We offer a wide selection of base supports and formats to purify antibodies using the Protein A/G ligand. Our portfolio is designed to meet small-scale (screening) up to large-scale (bioprocess) needs.

  • Multiple formats—magnetic beads, magnetic agarose beads, loose resins, spin columns and kits, FPLC cartridges, and 96-well filter plates enable antibody purification from microgram to kilogram scales
  • High performance—resins are designed to maximize protein yield and reduce background
  • Economical—pricing is similar to or better than our leading competitors

Choose the right Protein A/G product for antibody purification

Product links in the last row of each column

  Pierce Protein A/G
Magnetic Beads
Pierce Protein A/G
Magnetic Agarose Beads
Pierce Protein A/G
Agarose
Pierce Protein A/G Plus Agarose POROS MabCapture A/G Select
Bead or resin size 1 μm 10–40 μm 45–165 μm 45–165 μm 50 μm
Binding capacity ≥55–85 µg human IgG/mg beads ≥40 mg rabbit IgG/mL beads ≥7 mg human IgG/mL resin ≥50 mg human IgG/mL resin ≥22 mg/mL human IgG/mL resin
Maximum linear flow rate N/A N/A 700 cm/hr 700 cm/hr 3000 cm/hr
Support Magnetite-coated polymer Magnetite-embedded beaded agarose Crosslinked 6% beaded agarose Crosslinked 6% beaded agarose Polystyrene-divinylbenzene
Recommended application scale screening high-throughput batch batch, pilot batch, pilot batch, pilot, process
Formats available loose beads loose beads loose beads, pre-packed spin columns and kits, chromatography cartridges loose beads loose beads
Order product(s) 88802 78609 20421
(loose resin)
89930
(1 mL cartridge)
89931
(5 mL cartridge)
20423 82082