Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein A/G is a recombinant protein expressed in Escherichia coli (50.5 kDa; 6 IgG binding sites) and is recommended for purifying polyclonal IgG from many species. The POROS MabCapture A/G Select Resin is a new product that can be scaled up for bioprocess applications.

We offer a wide selection of base supports and formats to purify antibodies using the Protein A/G ligand. Our portfolio is designed to meet small-scale (screening) up to large-scale (bioprocess) needs.

  • Multiple formats—magnetic beads, magnetic agarose beads, loose resins, spin columns and kits, FPLC cartridges, and 96-well filter plates enable antibody purification from microgram to kilogram scales
  • High performance—resins are designed to maximize protein yield and reduce background
  • Economical—pricing is similar to or better than our leading competitors

Choose the right Protein A/G product for antibody purification

Product links in the last row of each column

 Pierce Protein A/G
Magnetic Beads
Pierce Protein A/G
Magnetic Agarose Beads
Pierce Protein A/G
Pierce Protein A/G Plus AgarosePOROS MabCapture A/G Select
Bead or resin size1 μm10–40 μm45–165 μm45–165 μm50 μm
Binding capacity≥55–85 µg human IgG/mg beads≥40 mg rabbit IgG/mL beads≥7 mg human IgG/mL resin≥50 mg human IgG/mL resin≥22 mg/mL human IgG/mL resin
Maximum linear flow rateN/AN/A700 cm/hr700 cm/hr3000 cm/hr
SupportMagnetite-coated polymerMagnetite-embedded beaded agaroseCrosslinked 6% beaded agaroseCrosslinked 6% beaded agarosePolystyrene-divinylbenzene
Recommended application scalescreeninghigh-throughput batchbatch, pilotbatch, pilotbatch, pilot, process
Formats availableloose beadsloose beadsloose beads, pre-packed spin columns and kits, chromatography cartridgesloose beadsloose beads
Order product(s)888027860920421
(loose resin)
(1 mL cartridge)
(5 mL cartridge)