Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein A ligand is recommended specifically for polyclonal IgG from rabbit, pig, dog, or cat serum. It binds the heavy chain constant region (Fc) of IgG (CH2-CH3 region). Native Protein A is purified from Staphylococcus aureus (46.7 kDa; 4 IgG-binding sites). The POROS™ MabCapture™ A Select Resin features a recombinant Protein A (rPA) ligand produced in yeast that can be scaled up for bioprocess applications.

We offer a wide selection of base supports and formats to purify antibodies using the Protein A ligand. Our portfolio is designed to meet small-scale (screening) up to large-scale (bioprocess) needs.

  • Multiple formats—magnetic beads, loose resin, spin columns and kits, FPLC cartridges, and 96-well filter plates enable antibody purification from microgram to kilogram scales
  • High performance—resins are designed to maximize protein yield and reduce background
  • Economical—pricing is similar to or better than our leading competitors

Choose the right Protein A product for antibody purification

Product links in the last row of each column

  Dynabeads
Protein A Magnetic Beads
Pierce
Protein A Agarose
Pierce
Protein A Plus Agarose
Pierce
Recombinant Protein A Agarose
POROS
MabCapture A Select
Bead or resin size 2.8 μm 45–165 μm 45–165 μm 45–165 μm 50 μm
Binding capacity 25–30 µg human IgG/mg beads 12–19 mg human IgG/mL resin 34 mg human IgG/mL resin 15 to 17 mg human IgG/mL resin ≥37 mg/mL human IgG/mL resin
Maximum linear flow rate N/A 700 cm/hr 700 cm/hr 700 cm/hr 3000 cm/hr
Support Monosized, super paramagnetic polystyrene beads Crosslinked 6% beaded agarose Crosslinked 6% beaded agarose Crosslinked 6% beaded agarose Polystyrene-divinylbenzene
Recommended application scale screening batch, pilot screening, batch batch batch, pilot, process
Formats available loose beads loose beads, chromatography cartridges loose beads, pre-packed spin columns and kits, 96-well filter plates loose beads loose beads
Order product(s) 10001D 20333
(loose resin)
88924
(1 mL cartridge)
89925
(5 mL cartridge)
44667
(spin kit)
22810
(loose resin)
45202
(spin plates)
20365 82079