Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein A ligand is recommended specifically for polyclonal IgG from rabbit, pig, dog, or cat serum. It binds the heavy chain constant region (Fc) of IgG (CH2-CH3 region). Native Protein A is purified from Staphylococcus aureus (46.7 kDa; 4 IgG-binding sites). The POROS MabCapture A resins feature a recombinant Protein A (rPA) ligand produced in yeast that can be scaled up for bioprocess applications.
The MabCaptureC™ High Capacity Protein A is a high-performance chromatography resin for capturing mAbs and recombinant Fc-tagged fusion proteins. It provides high specific binding capacity and enhanced alkaline stability characteristics under clean-in-place conditions (CIP), for a more robust purification and resin regeneration.
We offer a wide selection of base supports and formats to purify antibodies using the Protein A ligand. Our portfolio is designed to meet small-scale (screening) up to large-scale (bioprocess) needs.
Product links in the last row of each column
Pierce Protein A Agarose | Pierce Protein A Plus Agarose | Pierce Recombinant Protein A Agarose | MabCaptureC™ High Capacity Protein A, Alkaline-Stable | |
Bead or resin size | 45–165 μm | 45–165 μm | 45–165 μm | 75 μm |
Binding capacity | 12–19 mg human IgG/mL resin | 34 mg human IgG/mL resin | 15–17 mg human IgG/mL resin | 58 mg/ml at 5 min residence (80 mg/ml at 10 min residence) – 10% breakthrough |
Maximum linear flow rate | 700 cm/hr | 700 cm/hr | 700 cm/hr | 300 cm/hr or less (150 cm/hr recommended) |
Support | Crosslinked 6% beaded agarose | Crosslinked 6% beaded agarose | Crosslinked 6% beaded agarose | Highly cross-linked agarose |
Recommended application scale | batch, pilot | screening, batch | batch | batch, pilot, process |
Formats available | loose beads, chromatography cartridges | loose beads, pre-packed spin columns and kits, 96-well filter plates | loose beads | loose beads |
Order product(s) | 20333 (loose resin) 88924 (1 mL cartridge) 89925 (5 mL cartridge) 44667 (spin kit) | 22810 (loose resin) 45202 (spin plates) | 20365 | A53031, 10 mL 50% slurry A53033, 50 mL 50% slurry A53034, 200 mL 50% slurry 1963662250, 250 mL 50% slurry 196366201L, 1 L 50% slurry 196366205L, 5 L 50% slurry 196366210L, 10 L 50% slurry |
For Research Use Only. Not for use in diagnostic procedures.