Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein A ligand is recommended specifically for polyclonal IgG from rabbit, pig, dog, or cat serum. It binds the heavy chain constant region (Fc) of IgG (CH2-CH3 region). Native Protein A is purified from Staphylococcus aureus (46.7 kDa; 4 IgG-binding sites). The POROS™ MabCapture™ A Select Resin features a recombinant Protein A (rPA) ligand produced in yeast that can be scaled up for bioprocess applications.

We offer a wide selection of base supports and formats to purify antibodies using the Protein A ligand. Our portfolio is designed to meet small-scale (screening) up to large-scale (bioprocess) needs.

  • Multiple formats—magnetic beads, loose resin, spin columns and kits, FPLC cartridges, and 96-well filter plates enable antibody purification from microgram to kilogram scales
  • High performance—resins are designed to maximize protein yield and reduce background
  • Economical—pricing is similar to or better than our leading competitors

Choose the right Protein A product for antibody purification

Product links in the last row of each column

Protein A Magnetic Beads
Protein A Agarose
Protein A Plus Agarose
Recombinant Protein A Agarose
MabCapture A Select
Bead or resin size 2.8 μm 45–165 μm 45–165 μm 45–165 μm 50 μm
Binding capacity 25–30 µg human IgG/mg beads 12–19 mg human IgG/mL resin 34 mg human IgG/mL resin 15 to 17 mg human IgG/mL resin ≥37 mg/mL human IgG/mL resin
Maximum linear flow rate N/A 700 cm/hr 700 cm/hr 700 cm/hr 3000 cm/hr
Support Monosized, super paramagnetic polystyrene beads Crosslinked 6% beaded agarose Crosslinked 6% beaded agarose Crosslinked 6% beaded agarose Polystyrene-divinylbenzene
Recommended application scale screening batch, pilot screening, batch batch batch, pilot, process
Formats available loose beads loose beads, chromatography cartridges loose beads, pre-packed spin columns and kits, 96-well filter plates loose beads loose beads
Order product(s) 10001D 20333
(loose resin)
(1 mL cartridge)
(5 mL cartridge)
(spin kit)
(loose resin)
(spin plates)
20365 82079